CA2315812A1 - Detector having a transmission grating beam splitter for multi-wavelength sample analysis - Google Patents
Detector having a transmission grating beam splitter for multi-wavelength sample analysis Download PDFInfo
- Publication number
- CA2315812A1 CA2315812A1 CA002315812A CA2315812A CA2315812A1 CA 2315812 A1 CA2315812 A1 CA 2315812A1 CA 002315812 A CA002315812 A CA 002315812A CA 2315812 A CA2315812 A CA 2315812A CA 2315812 A1 CA2315812 A1 CA 2315812A1
- Authority
- CA
- Canada
- Prior art keywords
- order
- oth
- tagged
- detector
- dye
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
A detector for DNA sample identification is provided with a transmission grating beam splitter (TGBS) (38). The TGBS split fluoresced light (26) from a tagged DNA sample (22) into Oth order and 1st order components, both of which are detected on a two-dimensional detector array (31) of a CCD camera (30).
The DNA samples are tagged with four fluorescent dyes, one dye specific for each nucleotide, and all four dyes responding in slightly different manner to the same monochromatic excitation signal. The Oth and 1st order components are detected along a column of pixels in the detector array, and are spaced apart from one another. The 1st order component is received by pixels whose position (39b) relative to the Oth order component position (39a) depends on the frequency of fluorescence. Thus, the position at which the signal energy is detected on the array is indicative of the particular dye, and therefore, the corresponding nucleotide tagged by that dye. Monitoring signal energy at the Oth order pixel and selected 1st order pixels, provides a set of data from which one may then identify the particular nucleotide.
The DNA samples are tagged with four fluorescent dyes, one dye specific for each nucleotide, and all four dyes responding in slightly different manner to the same monochromatic excitation signal. The Oth and 1st order components are detected along a column of pixels in the detector array, and are spaced apart from one another. The 1st order component is received by pixels whose position (39b) relative to the Oth order component position (39a) depends on the frequency of fluorescence. Thus, the position at which the signal energy is detected on the array is indicative of the particular dye, and therefore, the corresponding nucleotide tagged by that dye. Monitoring signal energy at the Oth order pixel and selected 1st order pixels, provides a set of data from which one may then identify the particular nucleotide.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US6852797P | 1997-12-22 | 1997-12-22 | |
US60/068,527 | 1998-05-26 | ||
US09/084,236 | 1998-05-26 | ||
US09/084,236 US5998796A (en) | 1997-12-22 | 1998-05-26 | Detector having a transmission grating beam splitter for multi-wavelength sample analysis |
PCT/US1998/027259 WO1999032877A1 (en) | 1997-12-22 | 1998-12-22 | Detector having a transmission grating beam splitter for multi-wavelength sample analysis |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2315812A1 true CA2315812A1 (en) | 1999-07-01 |
CA2315812C CA2315812C (en) | 2006-04-18 |
Family
ID=26749060
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002315812A Expired - Fee Related CA2315812C (en) | 1997-12-22 | 1998-12-22 | Detector having a transmission grating beam splitter for multi-wavelength sample analysis |
Country Status (6)
Country | Link |
---|---|
US (2) | US5998796A (en) |
EP (1) | EP1049923A4 (en) |
JP (1) | JP2001527214A (en) |
AU (1) | AU2008599A (en) |
CA (1) | CA2315812C (en) |
WO (1) | WO1999032877A1 (en) |
Families Citing this family (52)
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US6225635B1 (en) * | 1998-08-07 | 2001-05-01 | The Regents Of The University Of California | System and method for optically locating microchannel positions |
JP3613032B2 (en) * | 1998-10-26 | 2005-01-26 | 株式会社日立製作所 | Capillary array electrophoresis device |
US6301377B1 (en) * | 1999-10-05 | 2001-10-09 | Large Scale Proteomics Corporation | Gel electrophoresis image warping |
US6630063B1 (en) * | 1999-10-06 | 2003-10-07 | I. Reich Family Limited Partnership | Uniform laser excitation and detection in capillary array electrophoresis system and method |
WO2001069211A1 (en) * | 2000-03-16 | 2001-09-20 | Spectrumedix Corporation | Multi-wavelength array reader for biological assay |
US6690467B1 (en) * | 2000-05-05 | 2004-02-10 | Pe Corporation | Optical system and method for optically analyzing light from a sample |
AU2001263286A1 (en) * | 2000-05-19 | 2001-12-03 | Iowa State University Research Foundation Inc. | High-throughput methods of distinguishing at least one molecule individually in a sample comprising multiple molecules and systems for use therein |
US6531041B1 (en) * | 2000-07-20 | 2003-03-11 | Symyx Technologies, Inc. | Multiplexed capillary electrophoresis system with rotatable photodetector |
WO2002061410A1 (en) * | 2000-09-01 | 2002-08-08 | Spectrumedix Corporation | System and method for temperature gradient capillary electrophoresis |
US6863791B1 (en) | 2000-09-11 | 2005-03-08 | Spectrumedix Llc | Method for in-situ calibration of electrophoretic analysis systems |
KR100371560B1 (en) * | 2000-12-14 | 2003-02-07 | 주식회사 디지탈바이오테크놀러지 | Dna detector |
US6978062B2 (en) | 2001-02-21 | 2005-12-20 | Ibsen Photonics A/S | Wavelength division multiplexed device |
US6842239B2 (en) * | 2001-08-10 | 2005-01-11 | Ibsen Photonics A/S | Alignment of multi-channel diffractive WDM device |
US7085492B2 (en) * | 2001-08-27 | 2006-08-01 | Ibsen Photonics A/S | Wavelength division multiplexed device |
US6652273B2 (en) * | 2002-01-14 | 2003-11-25 | The Procter & Gamble Company | Apparatus and method for controlling the temperature of manufacturing equipment |
US20050161329A1 (en) * | 2002-04-12 | 2005-07-28 | Hutterer Katariina M. | Multiplexed capillary electrophoresis systems |
US7872804B2 (en) | 2002-08-20 | 2011-01-18 | Illumina, Inc. | Encoded particle having a grating with variations in the refractive index |
US7900836B2 (en) | 2002-08-20 | 2011-03-08 | Illumina, Inc. | Optical reader system for substrates having an optically readable code |
US7901630B2 (en) | 2002-08-20 | 2011-03-08 | Illumina, Inc. | Diffraction grating-based encoded microparticle assay stick |
US7508608B2 (en) | 2004-11-17 | 2009-03-24 | Illumina, Inc. | Lithographically fabricated holographic optical identification element |
US7164533B2 (en) | 2003-01-22 | 2007-01-16 | Cyvera Corporation | Hybrid random bead/chip based microarray |
US7923260B2 (en) | 2002-08-20 | 2011-04-12 | Illumina, Inc. | Method of reading encoded particles |
US20100255603A9 (en) | 2002-09-12 | 2010-10-07 | Putnam Martin A | Method and apparatus for aligning microbeads in order to interrogate the same |
US7092160B2 (en) | 2002-09-12 | 2006-08-15 | Illumina, Inc. | Method of manufacturing of diffraction grating-based optical identification element |
US7238954B1 (en) | 2003-10-08 | 2007-07-03 | Fay Jr Theodore Denis | Optical external cavities having brewster angle wedges |
US7433123B2 (en) | 2004-02-19 | 2008-10-07 | Illumina, Inc. | Optical identification element having non-waveguide photosensitive substrate with diffraction grating therein |
JP4499476B2 (en) * | 2004-05-25 | 2010-07-07 | オリンパス株式会社 | Spectral image input apparatus and optical apparatus provided with the same |
WO2006020363A2 (en) | 2004-07-21 | 2006-02-23 | Illumina, Inc. | Method and apparatus for drug product tracking using encoded optical identification elements |
DE602005019791D1 (en) | 2004-11-16 | 2010-04-15 | Illumina Inc | METHOD AND DEVICE FOR READING CODED MICROBALLS |
JP4616051B2 (en) * | 2005-04-05 | 2011-01-19 | 株式会社日立ハイテクノロジーズ | Electrophoresis apparatus and electrophoresis method |
US7329860B2 (en) | 2005-11-23 | 2008-02-12 | Illumina, Inc. | Confocal imaging methods and apparatus |
US8288157B2 (en) | 2007-09-12 | 2012-10-16 | Plc Diagnostics, Inc. | Waveguide-based optical scanning systems |
US9423397B2 (en) | 2006-03-10 | 2016-08-23 | Indx Lifecare, Inc. | Waveguide-based detection system with scanning light source |
US9976192B2 (en) | 2006-03-10 | 2018-05-22 | Ldip, Llc | Waveguide-based detection system with scanning light source |
US9528939B2 (en) | 2006-03-10 | 2016-12-27 | Indx Lifecare, Inc. | Waveguide-based optical scanning systems |
DE102006015375A1 (en) * | 2006-04-03 | 2007-10-11 | Theta System Elektronik Gmbh | Device for measuring a spectral distribution of a printed product produced by a printing device |
US7830575B2 (en) | 2006-04-10 | 2010-11-09 | Illumina, Inc. | Optical scanner with improved scan time |
US7791013B2 (en) * | 2006-11-21 | 2010-09-07 | Illumina, Inc. | Biological microarray line scanning method and system |
US7813013B2 (en) * | 2006-11-21 | 2010-10-12 | Illumina, Inc. | Hexagonal site line scanning method and system |
WO2008116115A1 (en) * | 2007-03-20 | 2008-09-25 | Gary Bodily | Apparatus, system, and method for detecting chemicals |
FI120089B (en) * | 2008-01-23 | 2009-06-30 | Kone Corp | A method of installing a lift |
US20090229651A1 (en) * | 2008-03-14 | 2009-09-17 | Fay Jr Theodore Denis | Solar energy production system |
GB2461026B (en) * | 2008-06-16 | 2011-03-09 | Plc Diagnostics Inc | System and method for nucleic acids sequencing by phased synthesis |
EP2425286B1 (en) | 2009-04-29 | 2020-06-24 | Ldip, Llc | Waveguide-based detection system with scanning light source |
US20110068279A1 (en) * | 2009-08-11 | 2011-03-24 | Fay Jr Theodore Denis | Ultra dark field microscope |
JP2011058953A (en) * | 2009-09-10 | 2011-03-24 | Olympus Corp | Detector, optical apparatus with the same |
US8357281B2 (en) * | 2009-09-21 | 2013-01-22 | Advanced Analytical Technologies, Inc. | Multi-wavelength fluorescence detection system for multiplexed capillary electrophoresis |
WO2013076531A1 (en) * | 2011-11-23 | 2013-05-30 | Nokia Corporation | An apparatus and method comprising a beam splitter |
US10018566B2 (en) | 2014-02-28 | 2018-07-10 | Ldip, Llc | Partially encapsulated waveguide based sensing chips, systems and methods of use |
US10830639B2 (en) | 2014-09-25 | 2020-11-10 | Northwestern University | Devices, methods, and systems relating to super resolution imaging |
WO2016138427A1 (en) | 2015-02-27 | 2016-09-01 | Indx Lifecare, Inc. | Waveguide-based detection system with scanning light source |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3899213A (en) * | 1974-03-08 | 1975-08-12 | Us Transport | Airborne laser remote sensing system for the detection and identification of oil spills |
US3896312A (en) * | 1974-06-07 | 1975-07-22 | Christopher W Brown | Petroleum identification |
US4087685A (en) * | 1977-01-11 | 1978-05-02 | International Business Machines Corporation | Fluorescent microanalytical system and method for detecting and identifying organic materials |
DE3618605A1 (en) * | 1986-06-03 | 1987-12-10 | Europ Lab Molekularbiolog | DEVICE FOR DETECTING SUBSTANCES INCENTIVELY TO PHOTON EMISSION |
JP2550106B2 (en) * | 1987-10-30 | 1996-11-06 | 株式会社日立製作所 | Optical dispersion detection type electrophoretic device |
DE3843876A1 (en) * | 1988-12-24 | 1990-07-12 | Leitz Wild Gmbh | SPECTRAL MICROSCOPE WITH A PHOTOMETER |
JPH05231938A (en) * | 1991-02-07 | 1993-09-07 | Res Dev Corp Of Japan | Highly sensitive multiwavelength spectral apparatus |
US5303026A (en) * | 1991-02-26 | 1994-04-12 | The Regents Of The University Of California Los Alamos National Laboratory | Apparatus and method for spectroscopic analysis of scattering media |
JP3230890B2 (en) * | 1993-04-07 | 2001-11-19 | 株式会社日立製作所 | Electrophoretic separation analyzer |
US5483075A (en) * | 1994-11-01 | 1996-01-09 | Perkin-Elmer Corporation | Rotary scanning apparatus |
US6017434A (en) * | 1995-05-09 | 2000-01-25 | Curagen Corporation | Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments |
US5713364A (en) * | 1995-08-01 | 1998-02-03 | Medispectra, Inc. | Spectral volume microprobe analysis of materials |
DE69630011T2 (en) * | 1996-03-27 | 2004-04-01 | Agilent Technologies Inc., A Delaware Corp., Palo Alto | Method for the detection of sample substances and fluorescence spectrometer according to this method |
US5998796A (en) * | 1997-12-22 | 1999-12-07 | Spectrumedix Corporation | Detector having a transmission grating beam splitter for multi-wavelength sample analysis |
-
1998
- 1998-05-26 US US09/084,236 patent/US5998796A/en not_active Expired - Lifetime
- 1998-12-22 AU AU20085/99A patent/AU2008599A/en not_active Abandoned
- 1998-12-22 WO PCT/US1998/027259 patent/WO1999032877A1/en not_active Application Discontinuation
- 1998-12-22 JP JP2000525746A patent/JP2001527214A/en active Pending
- 1998-12-22 CA CA002315812A patent/CA2315812C/en not_active Expired - Fee Related
- 1998-12-22 EP EP98964854A patent/EP1049923A4/en not_active Withdrawn
-
1999
- 1999-11-29 US US09/449,418 patent/US6118127A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
WO1999032877A1 (en) | 1999-07-01 |
US5998796A (en) | 1999-12-07 |
EP1049923A1 (en) | 2000-11-08 |
CA2315812C (en) | 2006-04-18 |
US6118127A (en) | 2000-09-12 |
JP2001527214A (en) | 2001-12-25 |
EP1049923A4 (en) | 2002-04-10 |
AU2008599A (en) | 1999-07-12 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |
Effective date: 20121224 |
|
MKLA | Lapsed |
Effective date: 20121224 |