CA2315812A1 - Detector having a transmission grating beam splitter for multi-wavelength sample analysis - Google Patents

Detector having a transmission grating beam splitter for multi-wavelength sample analysis Download PDF

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Publication number
CA2315812A1
CA2315812A1 CA002315812A CA2315812A CA2315812A1 CA 2315812 A1 CA2315812 A1 CA 2315812A1 CA 002315812 A CA002315812 A CA 002315812A CA 2315812 A CA2315812 A CA 2315812A CA 2315812 A1 CA2315812 A1 CA 2315812A1
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CA
Canada
Prior art keywords
order
oth
tagged
detector
dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002315812A
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French (fr)
Other versions
CA2315812C (en
Inventor
Changsheng Liu
Qingbo Li
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Applied Biosystems LLC
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Individual
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Publication date
Application filed by Individual filed Critical Individual
Publication of CA2315812A1 publication Critical patent/CA2315812A1/en
Application granted granted Critical
Publication of CA2315812C publication Critical patent/CA2315812C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means

Abstract

A detector for DNA sample identification is provided with a transmission grating beam splitter (TGBS) (38). The TGBS split fluoresced light (26) from a tagged DNA sample (22) into Oth order and 1st order components, both of which are detected on a two-dimensional detector array (31) of a CCD camera (30).
The DNA samples are tagged with four fluorescent dyes, one dye specific for each nucleotide, and all four dyes responding in slightly different manner to the same monochromatic excitation signal. The Oth and 1st order components are detected along a column of pixels in the detector array, and are spaced apart from one another. The 1st order component is received by pixels whose position (39b) relative to the Oth order component position (39a) depends on the frequency of fluorescence. Thus, the position at which the signal energy is detected on the array is indicative of the particular dye, and therefore, the corresponding nucleotide tagged by that dye. Monitoring signal energy at the Oth order pixel and selected 1st order pixels, provides a set of data from which one may then identify the particular nucleotide.
CA002315812A 1997-12-22 1998-12-22 Detector having a transmission grating beam splitter for multi-wavelength sample analysis Expired - Fee Related CA2315812C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US6852797P 1997-12-22 1997-12-22
US60/068,527 1997-12-22
US09/084,236 1998-05-26
US09/084,236 US5998796A (en) 1997-12-22 1998-05-26 Detector having a transmission grating beam splitter for multi-wavelength sample analysis
PCT/US1998/027259 WO1999032877A1 (en) 1997-12-22 1998-12-22 Detector having a transmission grating beam splitter for multi-wavelength sample analysis

Publications (2)

Publication Number Publication Date
CA2315812A1 true CA2315812A1 (en) 1999-07-01
CA2315812C CA2315812C (en) 2006-04-18

Family

ID=26749060

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002315812A Expired - Fee Related CA2315812C (en) 1997-12-22 1998-12-22 Detector having a transmission grating beam splitter for multi-wavelength sample analysis

Country Status (6)

Country Link
US (2) US5998796A (en)
EP (1) EP1049923A4 (en)
JP (1) JP2001527214A (en)
AU (1) AU2008599A (en)
CA (1) CA2315812C (en)
WO (1) WO1999032877A1 (en)

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Also Published As

Publication number Publication date
WO1999032877A1 (en) 1999-07-01
US6118127A (en) 2000-09-12
JP2001527214A (en) 2001-12-25
EP1049923A4 (en) 2002-04-10
US5998796A (en) 1999-12-07
AU2008599A (en) 1999-07-12
EP1049923A1 (en) 2000-11-08
CA2315812C (en) 2006-04-18

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