CA2286183A1 - Compositions and methods for inhibiting light-induced inactivation of biological reagents - Google Patents
Compositions and methods for inhibiting light-induced inactivation of biological reagents Download PDFInfo
- Publication number
- CA2286183A1 CA2286183A1 CA002286183A CA2286183A CA2286183A1 CA 2286183 A1 CA2286183 A1 CA 2286183A1 CA 002286183 A CA002286183 A CA 002286183A CA 2286183 A CA2286183 A CA 2286183A CA 2286183 A1 CA2286183 A1 CA 2286183A1
- Authority
- CA
- Canada
- Prior art keywords
- compositions
- methods
- biological reagent
- biological reagents
- inhibiting light
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/80—Fluorescent dyes, e.g. rhodamine
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/13—Tracers or tags
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/2525—Stabilizing or preserving
Abstract
Disclosed is a fluorescent conjugate comprising a biological reagent, a fluorescent molecule, and a means for impeding phototoxic degradation of the biological reagent. The impeding means can be a cross-linking substance having a long molecular distance, whereby the cross-linking substance links the fluorescent molecule and the biological reagent; a quencher of singlet oxygen; a quencher of a free radical; or a combination thereof. Also disclosed is a solution comprising a fluorescent conjugate of a biological reagent and a fluorescent molecule together with an oxygen depleting system.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/837,309 US6544797B1 (en) | 1997-04-09 | 1997-04-09 | Compositions and methods for inhibiting light-induced inactivation of biological reagents |
US08/837,309 | 1997-04-09 | ||
PCT/US1998/006793 WO1998045705A2 (en) | 1997-04-09 | 1998-04-06 | Compositions and methods for inhibiting light-induced inactivation of biological reagents |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2286183A1 true CA2286183A1 (en) | 1998-10-15 |
CA2286183C CA2286183C (en) | 2008-11-18 |
Family
ID=25274130
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002286183A Expired - Lifetime CA2286183C (en) | 1997-04-09 | 1998-04-06 | Compositions and methods for inhibiting light-induced inactivation of biological reagents |
Country Status (8)
Country | Link |
---|---|
US (2) | US6544797B1 (en) |
EP (1) | EP0972194B1 (en) |
JP (1) | JP2001519908A (en) |
AT (1) | ATE244404T1 (en) |
AU (1) | AU6796598A (en) |
CA (1) | CA2286183C (en) |
DE (1) | DE69816051T2 (en) |
WO (1) | WO1998045705A2 (en) |
Families Citing this family (37)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2799839A1 (en) * | 2000-02-02 | 2001-04-20 | Commissariat Energie Atomique | Determining antioxidant capacity of compounds, useful for assessing them for e.g. pharmaceutical application, based on inhibition of covalent reaction mediated by reactive oxygen species |
US6800068B1 (en) * | 2001-10-26 | 2004-10-05 | Radiant Medical, Inc. | Intra-aortic balloon counterpulsation with concurrent hypothermia |
US11008359B2 (en) | 2002-08-23 | 2021-05-18 | Illumina Cambridge Limited | Labelled nucleotides |
US20040241880A1 (en) * | 2003-05-30 | 2004-12-02 | Leproust Eric M. | Ligand array assays having reduced fluorescent dye degradation and compositions for practicing the same |
US20040241742A1 (en) * | 2003-05-30 | 2004-12-02 | Peck Bill J. | Ligand array processing methods that include a low surface tension fluid deposition step and compositions for practicing the same |
GB0424294D0 (en) * | 2004-11-03 | 2004-12-01 | Elam T Ltd | Buffer layer |
EP1828412B2 (en) * | 2004-12-13 | 2019-01-09 | Illumina Cambridge Limited | Improved method of nucleotide detection |
GB0427236D0 (en) | 2004-12-13 | 2005-01-12 | Solexa Ltd | Improved method of nucleotide detection |
US7998717B2 (en) | 2005-12-02 | 2011-08-16 | Pacific Biosciences Of California, Inc. | Mitigation of photodamage in analytical reactions |
US20100311186A1 (en) * | 2006-07-28 | 2010-12-09 | Biosite Incorporated | Devices and methods for performing receptor binding assays using magnetic particles |
US20090043109A1 (en) * | 2007-08-07 | 2009-02-12 | Spangler Brenda D | Asymmetric one- and two-photon fluorophores for simultaneous detection of multiple analytes using a common excitation source |
US20090062386A1 (en) * | 2007-08-30 | 2009-03-05 | Clean Earth Technologies, Llc | Simulants of Toxants for Training and Testing |
US20090057622A1 (en) * | 2007-08-31 | 2009-03-05 | Clean Earth Technologies, Llc | Simulant of Radiological Contamination |
EP2252625B1 (en) * | 2008-02-07 | 2017-12-20 | Pacific Biosciences of California, Inc. | Cis reactive oxygen quenchers integrated into linkers |
WO2009114182A1 (en) | 2008-03-13 | 2009-09-17 | Pacific Biosciences Of California, Inc. | Labeled reactants and their uses |
US8388982B2 (en) * | 2008-11-19 | 2013-03-05 | Pacific Biosciences Of California, Inc. | Photo-induced damage mitigating agents and preparation and methods of use thereof |
DE102009010563A1 (en) | 2009-02-16 | 2010-08-26 | Matthias W. Engel | Device for the detection of analytes in body fluids |
GB0913258D0 (en) | 2009-07-29 | 2009-09-02 | Dynex Technologies Inc | Reagent dispenser |
US9523701B2 (en) | 2009-07-29 | 2016-12-20 | Dynex Technologies, Inc. | Sample plate systems and methods |
WO2012021733A2 (en) | 2010-08-12 | 2012-02-16 | Pacific Biosciences Of California, Inc. | Photodamage mitigation compounds and systems |
US8906612B2 (en) | 2010-08-25 | 2014-12-09 | Pacific Biosciences Of California, Inc. | Scaffold-based polymerase enzyme substrates |
US8486717B2 (en) | 2011-01-18 | 2013-07-16 | Symbolics, Llc | Lateral flow assays using two dimensional features |
US8835000B2 (en) * | 2011-12-23 | 2014-09-16 | General Electric Company | High-density fluorescent dye clusters |
DE102012102082B3 (en) * | 2012-03-13 | 2013-03-21 | Thyssenkrupp Rasselstein Gmbh | A method of treating a metal-coated steel strip or sheet with an aftertreatment agent and a steel strip or sheet provided with a metal coating. |
US9315864B2 (en) | 2012-05-18 | 2016-04-19 | Pacific Biosciences Of California, Inc. | Heteroarylcyanine dyes with sulfonic acid substituents |
US10458915B2 (en) | 2012-05-18 | 2019-10-29 | Pacific Biosciences Of California, Inc. | Heteroarylcyanine dyes |
US9874556B2 (en) | 2012-07-18 | 2018-01-23 | Symbolics, Llc | Lateral flow assays using two dimensional features |
US20140072959A1 (en) | 2012-09-12 | 2014-03-13 | Force Diagnostics, Inc. | Rapid tests for insurance underwriting |
CN105051542A (en) * | 2012-10-31 | 2015-11-11 | 阿斯图德医疗有限公司 | Quantitative lateral flow assay |
CN105102980B (en) | 2013-02-26 | 2017-11-03 | 阿斯图特医药公司 | Lateral flow assays with strip keeper |
CN108864232B (en) | 2013-03-15 | 2022-01-04 | 伊鲁米纳剑桥有限公司 | Modified nucleosides or nucleotides |
CN108051590B (en) | 2013-09-13 | 2020-12-11 | Symbolics有限责任公司 | Lateral flow assay using two-dimensional test and control signal readout modes |
JP6286966B2 (en) * | 2013-09-18 | 2018-03-07 | コニカミノルタ株式会社 | RESIN PARTICLE FOR FLUORESCENT LABELING, PROCESS FOR PRODUCING THE SAME, AND FLUORESCENT LABELING AGENT FOR IMMUNO STAINING CONTAINING THE SAME |
US20180106799A1 (en) | 2015-04-06 | 2018-04-19 | Bludiagnostics, Inc. | A test device for detecting an analyte in a saliva sample and method of use |
EP3676612A4 (en) * | 2017-08-31 | 2021-05-26 | Agilent Technologies, Inc. | Composition for stabilization of antibody formulations containing percp |
US20200363406A1 (en) * | 2017-10-26 | 2020-11-19 | The University Of Houston System | Highly-specific assays |
WO2021213321A1 (en) | 2020-04-20 | 2021-10-28 | Mgi Tech Co., Ltd. | Dna protection agent in dna imaging buffer |
Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4283382A (en) | 1977-12-28 | 1981-08-11 | Eastman Kodak Company | Fluorescent labels comprising rare earth chelates |
US4351760A (en) | 1979-09-07 | 1982-09-28 | Syva Company | Novel alkyl substituted fluorescent compounds and polyamino acid conjugates |
US4420568A (en) | 1980-07-30 | 1983-12-13 | Abbott Laboratories | Fluorescent polarization immunoassay utilizing substituted triazinylaminofluoresceins |
US5075215A (en) | 1984-06-11 | 1991-12-24 | Dreyer William J | Use of daylight fluorescent pigments for tagging biological molecules |
US4868132A (en) | 1987-02-03 | 1989-09-19 | Abbott Laboratories | Fluorescence polarization immunoassay for amphetamine/methamphetamine |
US4876190A (en) | 1987-10-21 | 1989-10-24 | Becton Dickinson & Company | Peridinin-chlorophyll complex as fluorescent label |
DE3919393A1 (en) * | 1989-06-14 | 1990-12-20 | Hoechst Ag | METHOD FOR STABILIZING SOLUBLE-IMMOBILIZED BIOLOGICAL ACTIVE SUBSTANCES |
DE4004296A1 (en) * | 1990-02-13 | 1991-08-14 | Hoechst Ag | IMPROVED MARKED HAPTENE, METHOD FOR THE PRODUCTION THEREOF AND USE OF THIS MARKED HAPTENE IN IMMUNOASSAYS |
US5747334A (en) * | 1990-02-15 | 1998-05-05 | The University Of North Carolina At Chapel Hill | Random peptide library |
US5227487A (en) * | 1990-04-16 | 1993-07-13 | Molecular Probes, Inc. | Certain tricyclic and pentacyclic-hetero nitrogen rhodol dyes |
DE69132750T2 (en) * | 1990-05-15 | 2002-06-20 | Hyperion Inc | FLUORESCENT PORPHYRIN AND FLUORESCENT PHTHALOCYANIN-POLYETHYLENE GLYCOL, POLYOL AND SACCHARID DERIVATIVES AS FLUORESCENT PROBE |
DE69125992T2 (en) * | 1990-09-14 | 1997-08-21 | Tosoh Corp | Method and kit for immunoassay |
ATE177841T1 (en) * | 1991-04-10 | 1999-04-15 | Biosite Diagnostics Inc | CROSSTALK OR CROSSTALK INHIBITORS AND THEIR USE |
US5230836A (en) * | 1991-06-20 | 1993-07-27 | Kalamazoo Holdings, Inc. | Low micron-sized ascorbic acid particles, especially a suspension thereof in a medium in which they are insoluble, and the use thereof as an antioxidant for mediums in which the particles remain insoluble |
ATE162892T1 (en) * | 1992-07-31 | 1998-02-15 | Behringwerke Ag | PHOTOACTIVABLE CHEMILUMINIZING MATRICES |
US6251687B1 (en) * | 1993-09-24 | 2001-06-26 | Biosite Diagnostics, Inc. | Fluorescence energy transfer and intramolecular energy transfer in particles using novel compounds |
US6238931B1 (en) * | 1993-09-24 | 2001-05-29 | Biosite Diagnostics, Inc. | Fluorescence energy transfer in particles |
EP0796108A2 (en) * | 1994-12-06 | 1997-09-24 | Ryan Pharmaceuticals, Inc. | Water soluble ubiquinone compositions, prodrugs, and methods relating thereto |
US5650512A (en) * | 1995-06-07 | 1997-07-22 | Systemix | Fluorescent labeling reagents |
US5798276A (en) * | 1995-06-07 | 1998-08-25 | Molecular Probes, Inc. | Reactive derivatives of sulforhodamine 101 with enhanced hydrolytic stability |
-
1997
- 1997-04-09 US US08/837,309 patent/US6544797B1/en not_active Expired - Lifetime
-
1998
- 1998-04-06 WO PCT/US1998/006793 patent/WO1998045705A2/en active IP Right Grant
- 1998-04-06 EP EP98913406A patent/EP0972194B1/en not_active Expired - Lifetime
- 1998-04-06 AT AT98913406T patent/ATE244404T1/en not_active IP Right Cessation
- 1998-04-06 DE DE69816051T patent/DE69816051T2/en not_active Expired - Lifetime
- 1998-04-06 JP JP54300498A patent/JP2001519908A/en active Pending
- 1998-04-06 AU AU67965/98A patent/AU6796598A/en not_active Abandoned
- 1998-04-06 CA CA002286183A patent/CA2286183C/en not_active Expired - Lifetime
-
2003
- 2003-01-07 US US10/338,182 patent/US7588908B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
JP2001519908A (en) | 2001-10-23 |
ATE244404T1 (en) | 2003-07-15 |
CA2286183C (en) | 2008-11-18 |
US7588908B2 (en) | 2009-09-15 |
EP0972194A2 (en) | 2000-01-19 |
DE69816051D1 (en) | 2003-08-07 |
US6544797B1 (en) | 2003-04-08 |
WO1998045705A3 (en) | 1998-12-30 |
US20030129642A1 (en) | 2003-07-10 |
EP0972194B1 (en) | 2003-07-02 |
AU6796598A (en) | 1998-10-30 |
WO1998045705A2 (en) | 1998-10-15 |
DE69816051T2 (en) | 2004-05-19 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKEX | Expiry |
Effective date: 20180406 |