CA2280741A1 - Dry powder cells and cell culture reagents and methods of production thereof - Google Patents

Dry powder cells and cell culture reagents and methods of production thereof Download PDF

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Publication number
CA2280741A1
CA2280741A1 CA002280741A CA2280741A CA2280741A1 CA 2280741 A1 CA2280741 A1 CA 2280741A1 CA 002280741 A CA002280741 A CA 002280741A CA 2280741 A CA2280741 A CA 2280741A CA 2280741 A1 CA2280741 A1 CA 2280741A1
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Prior art keywords
powder
cell
nutritive medium
supplement
serum
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Granted
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CA002280741A
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French (fr)
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CA2280741C (en
Inventor
Richard Fike
William Whitford
William Biddle
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Life Technologies Corp
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Individual
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Priority to CA2798535A priority Critical patent/CA2798535C/en
Publication of CA2280741A1 publication Critical patent/CA2280741A1/en
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Publication of CA2280741C publication Critical patent/CA2280741C/en
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Expired - Lifetime legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/0025Culture media for plant cell or plant tissue culture

Abstract

The present invention relates generally to nutritive medium, medi0m supplement, media subgroup and buffer formulations. Specifically, the present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention is particularly directed to methods of production of these media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations. The invention also relates to methods of producing sterile powdered media, media supplement (particularly powdered sera such as powdered FBS, powdered transferrin, powdered insulin, powdered organ extracts (such as bovine brain or pituitary extracts), powdered growth factors (such as EGF, FGF, etc.) and the like), media subgroup and buffer formulations. In a particularly preferred aspect, the invention relates to such methods wherein the sterilization is accomplished by gamma irradiation.
The invention also relates to methods for producing dry cell powders, comprising spray-drying a cell suspension. The invention also relates to cell, media, media supplement, media subgroup and buffer powders produced by these methods.

Claims (91)

1. A method of producing a nutritive medium powder, said method comprising agglomerating a dry powder nutritive medium with a solvent.
2. A method of producing a nutritive medium supplement powder, said method comprising agglomerating a dry powder nutritive medium supplement with a solvent.
3 . A method of producing a nutritive medium subgroup powder, said method comprising agglomerating a dry powder nutritive medium subgroup with a solvent.
4. A method of producing a buffer powder, said method comprising agglomerating a dry powder buffer with a solvent.
5. A method of producing a nutritive medium powder, said method comprising spray-drying a liquid nutritive medium.
6. A method of producing a nutritive medium supplement powder, said method comprising spray-drying a liquid nutritive medium supplement.
7. A method of producing a nutritive medium subgroup powder, said method comprising spray-drying a liquid nutritive medium subgroup.
8. A method of producing a buffer powder, said method comprising spray-drying a liquid buffer solution.
9. The method of any one of claims 5-8, further comprising agglomerating said powder with a solvent.
10. The method of any one of claims 5-8, wherein said spray-drying is performed under conditions of controlled temperature and humidity.
11. The method of any one of claims 1-8, further comprising packaging said powder.
12. The method of any one of claims 1-8, further comprising sterilizing said powder.
13. The method of claim 12, wherein said sterilization is performed after packaging said powder.
14. The method of claim 12, wherein said sterilization is accomplished by irradiation of said powder with gamma rays until said powder is rendered substantially sterile.
15. The method of claim 1 or claim 5, further comprising adding at least one medium supplement to said agglomerated nutritive medium by spray-drying said supplement onto said agglomerated medium.
16. The method of claim 1 or claim 5, further comprising adding an acid or base to said nutritive medium by agglomerating said acid or base into said nutritive medium.
17. The method of claim 1 or claim 5, wherein said nutritive medium powder is a cell culture medium powder selected from the group consisting of a bacterial cell culture medium powder, a yeast cell culture medium powder, a plant cell culture medium powder and an animal cell culture medium powder.
18. The method of claim 1 or claim 5, wherein said nutritive medium comprises one or more ingredients selected from the group consisting of serum, L-glutamine, insulin, transferrin, a lipid, a cytokine, a neurotransmitter and a buffer.
19. The method of claim 2, wherein said nutritive medium supplement is serum.
20. The method of claim 3, wherein said nutritive medium supplement is serum.
21. The method of claim 15, wherein said nutritive medium supplement is serum.
22. The method of any one of claims 19-21, wherein said serum is bovine serum or human serum.
23. The method of claim 22, wherein said bovine serum is fetal bovine serum or calf serum.
24. The method of claim 18, wherein said buffer is sodium bicarbonate.
25. The method of claim 4 or claim 8, wherein said buffer powder is a buffered saline powder.
26. The method of claim 25, wherein said buffered saline powder is a phosphate-buffered saline powder or a Tris-buffered saline powder.
27. A nutritive medium powder prepared according to the method of claim 1.
28. A nutritive medium powder prepared according to the method of claim 5 .
29. A nutritive medium powder prepared according to the method of claim 9.
30. A nutritive medium powder prepared according to the method of claim 16.
31. An automatically pH-adjusting nutritive medium powder.
32. The nutritive medium powder of claim 31, wherein said powder comprises at least one buffer salt.
33. The nutritive medium powder of claim 32, wherein said buffer salt is sodium bicarbonate.
34. The nutritive medium powder of any one of claims 27-31, wherein said nutritive medium powder comprises at least one buffer salt.
35. The nutritive medium powder of claim 34, wherein said buffer salt is sodium bicarbonate.
36. The nutritive medium powder of any one of claims 27-31, wherein said nutritive medium has a pH of between 7.1-7.5 when said medium is reconstituted with a solvent.
37. A nutritive medium supplement powder prepared according to the method of claim 2.
38. A nutritive medium supplement powder prepared according to the method of claim 3.
39. A nutritive medium subgroup powder prepared according to the method of claim 3.
40. A nutritive medium subgroup powder prepared according to the method of claim 7.
41. A nutritive medium subgroup powder prepared according to the method of claim 9.
42. A buffer powder prepared according to the method of claim 4
43. A buffer powder prepared according to the method of claim 8.
44. The nutritive medium supplement powder of claim 37 or claim 38, wherein said nutritive medium supplement is serum.
45. The nutritive medium supplement powder of claim 44, wherein said serum is bovine serum or human serum.
46. The nutritive medium supplement powder of claim 45, wherein said bovine serum is fetal bovine serum or calf serum.
47. The nutritive medium supplement powder of claim 37 or claim 38, wherein said nutritive medium supplement powder comprises one or more nutritive medium components.
48. A method of culturing a cell comprising reconstituting the nutritive medium powder of any one of claims 27-31 with a solvent to form a liquid solution and contacting the cell with said liquid solution under conditions favoring the cultivation of the cell.
49. The method of claim 48, wherein said solvent comprises serum or water.
50. The method of claim 48, wherein said cell is selected from the group consisting of a bacterial cell, a yeast cell, a plant cell and an animal cell.
51. The method of claim 50, wherein said animal cell is an insect cell, a nematode cell or a mammalian cell.
52. The method of claim 51, wherein said mammalian cell is selected from the group consisting of a CHO cell, a COS cell, a VERO cell, a BHK cell, an AE-1 cell, an SP2/0 cell, an L5.1 cell, a hybridoma cell, and a human cell.
53. The method of claim 48, wherein said cell is an anchorage-dependent cell.
54. The method of claim 48, wherein said cell is an anchorage-independent cell.
55. The method of claim 48 wherein said cell is selected from the group consisting of a normal cell, a diseased cell, a transformed cell, a mutant cell, a somatic cell, a germ cell, a stem cell, a precursor cell and an embryonic cell.
56. A method of producing a sterile nutritive medium supplement powder, comprising:
(a) obtaining a nutritive medium supplement powder to be sterilized; and (b) sterilizing said powder by irradiation of said powder with y rays until said powder is rendered sterile.
57. The method of claim 56, wherein said nutritive medium supplement powder is prepared by lyophilizing a liquid nutritive medium supplement.
58. The method of claim 56, wherein said nutritive medium supplement powder is prepared by spray-drying a liquid nutritive medium supplement.
59. The method of claim 56, wherein said nutritive medium supplement powder is prepared by agglomerating a powdered nutritive medium supplement.
60. A sterile nutritive medium supplement powder prepared according to the method of claim 56.
61. The sterile nutritive medium supplement powder of claim 60, wherein said powder is selected from the group consisting of a serum powder, a cytokine powder, an insulin powder, a lipid powder, an animal organ extract powder, an animal gland extract powder, an animal tissue extract powder, an enzyme powder and a transferrin powder.
62. The sterile nutritive medium supplement powder of claim 60, wherein said powder is a serum powder.
63. The sterile nutritive medium supplement powder of claim 61 or claim 62, wherein said serum powder is a bovine serum powder or a human serum powder.
64. The sterile nutritive medium supplement powder of claim 63, wherein said bovine serum powder is a fetal bovine serum powder or a calf serum powder.
65. The sterile nutritive medium supplement powder of claim 61, wherein said cytokine powder is a growth factor powder, an interleukin powder, a colony-stimulating factor powder or an interferon powder.
66. The sterile nutritive medium supplement powder of claim 61, wherein said lipid powder is a phospholipid powder, a sphingolipid powder, a fatty acid powder or a cholesterol powder.
67. The sterile nutritive medium supplement powder of claim 61, wherein said animal gland extract powder is a bovine pituitary extract powder.
68. The sterile nutritive medium supplement powder of claim 61, wherein said enzyme powder is a trypsin powder, a collagenase powder, a pancreatinin powder or a dispase powder.
69. The sterile nutritive medium supplement powder of claim 60, wherein said powder is a transferrin powder.
70. A kit for use in the cultivation of a cell, said kit comprising one or more containers wherein a first container contains the powder of any one of claims 27-31, 37-43 or 60.
71. The kit of claim 70, further comprising one or more additional containers containing one or more cells.
72. The kit of claim 70, further comprising one or more additional containers containing one or more solvents.
73. A method of producing a dry cell powder comprising at least one cell, said method comprising:
(a) obtaining a cell to be dried;
(b) forming a cell suspension by suspending said cell in an aqueous solution; and (c) producing a powder by spray-drying said cell suspension.
74. The method of claim 73, further comprising contacting said cell with at least one stabilizing or preserving compound.
75. The method of claim 74, wherein said stabilizing or preserving compound is a polysaccharide.
76. The method of claim 75, wherein said polysaccharide is trehalose.
77. The method of claim 74, wherein said cell is contacted with the stabilizing or preserving compound by spray-drying or agglomerating the compound onto said dry cell powder.
78. The method of claim 73, wherein said aqueous solution comprises one or more components selected from the group consisting of a buffer, a salt, a nutritive medium component and a nutritive medium supplement.
79. The method of claim 73, further comprising agglomerating said dry cell powder with a solvent.
80. The method of claim 79, wherein said solvent is selected from the group consisting of a buffer solution, a salt solution, a nutritive medium supplement solution, a nutritive medium solution, and a combination thereof.
81. The method of claim 78 or claim 80, wherein said nutritive medium supplement is serum.
82. The method of claim 81, wherein said serum is bovine serum or human serum.
83. The method of claim 82, wherein said bovine serum is fetal bovine serum or calf serum.
84. The method of claim 73, wherein said cell is a bacterial cell, a yeast cell, an animal cell or a plant cell.
85. The method of claim 84, wherein said animal cell is an insect cell, a nematode cell or a mammalian cell.
86. The method of claim 85, wherein said mammalian cell is a human cell.
87. The method of claim 85, wherein said mammalian cell is a non-human cell.
88. The method of claim 73, wherein said cell is selected from the group consisting of a normal cell, a diseased cell, a transformed cell, a mutant cell, a somatic cell, a germ cell, a stem cell, a precursor cell and an embryonic cell.
89. The method of claim 73, wherein said cell is an anchorage-dependent cell.
90. The method of claim 73, wherein said cell is an anchorage-independent cell.
91. A dry cell powder prepared according to the method of claim 73.
CA2280741A 1997-02-14 1998-02-17 Dry powder cells and cell culture reagents and methods of production thereof Expired - Lifetime CA2280741C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2798535A CA2798535C (en) 1997-02-14 1998-02-17 Dry powder cells and cell culture reagents and methods of production thereof

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US4031497P 1997-02-14 1997-02-14
US60/040,314 1997-02-14
US5871697P 1997-09-12 1997-09-12
US60/058,716 1997-09-12
US6219297P 1997-10-16 1997-10-16
US60/062,192 1997-10-16
PCT/US1998/002829 WO1998036051A1 (en) 1997-02-14 1998-02-17 Dry powder cells and cell culture reagents and methods of production thereof

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CA2280741A1 true CA2280741A1 (en) 1998-08-20
CA2280741C CA2280741C (en) 2013-04-09

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EP (4) EP1012234A4 (en)
JP (8) JP4557311B2 (en)
AT (1) ATE507283T1 (en)
AU (1) AU6654998A (en)
CA (2) CA2798535C (en)
DE (1) DE69842243D1 (en)
DK (3) DK2281873T3 (en)
ES (1) ES2516790T3 (en)
WO (1) WO1998036051A1 (en)

Families Citing this family (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5362442A (en) * 1993-07-22 1994-11-08 2920913 Canada Inc. Method for sterilizing products with gamma radiation
US20040067157A1 (en) * 1993-07-22 2004-04-08 Clearant, Inc. Methods for sterilizing biological materials
US6051256A (en) * 1994-03-07 2000-04-18 Inhale Therapeutic Systems Dispersible macromolecule compositions and methods for their preparation and use
EP0891419A4 (en) * 1996-03-12 2000-03-01 Life Technologies Inc Hematopoietic cell culture nutrient supplement
US20030203036A1 (en) * 2000-03-17 2003-10-30 Gordon Marc S. Systems and processes for spray drying hydrophobic drugs with hydrophilic excipients
JP2001512310A (en) 1997-02-12 2001-08-21 ライフ テクノロジーズ,インコーポレイテッド Method for freeze-drying competent cells
US6383810B2 (en) * 1997-02-14 2002-05-07 Invitrogen Corporation Dry powder cells and cell culture reagents and methods of production thereof
US20060003447A1 (en) * 2003-12-30 2006-01-05 Richard Fike Dry powder cells and cell culture reagents and methods of production thereof
US20060003448A1 (en) * 2003-12-30 2006-01-05 Richard Fike Dry powder cells and cell culture reagents and methods of production thereof
JP2002519009A (en) * 1998-06-30 2002-07-02 インビトロゲン・コーポレーション Methods for attenuating exogenous factors and toxins and cell culture reagents produced thereby.
WO2000011953A1 (en) * 1998-09-01 2000-03-09 Penn State Research Foundation Method and apparatus for aseptic growth or processing of biomass
AT409379B (en) 1999-06-02 2002-07-25 Baxter Ag MEDIUM FOR PROTEIN- AND SERUM-FREE CELL CULTURE
FR2804326B1 (en) * 2000-01-27 2002-10-18 Ellipse Pharmaceuticals STERILIZATION PROCESS BY HIGH PRESSURE TREATMENT OF SENSITIVE ACTIVE INGREDIENTS
US20040086420A1 (en) * 2000-03-23 2004-05-06 Macphee Martin J. Methods for sterilizing serum or plasma
EA005977B1 (en) * 2000-03-23 2005-08-25 Клиарант, Инк. Methods for sterilizing biological materials
IL136232A0 (en) * 2000-05-18 2001-05-20 Bar Ilan University Res Author Measurements of enzymatic activity in a single, individual cell in population
US7575761B2 (en) * 2000-06-30 2009-08-18 Novartis Pharma Ag Spray drying process control of drying kinetics
WO2002009669A2 (en) * 2000-08-01 2002-02-07 Inhale Therapeutic Systems, Inc. Apparatus and process to produce particles having a narrow size distribution and particles made thereby
KR100385713B1 (en) * 2000-08-30 2003-05-27 사회복지법인삼성생명공익재단(삼성서울병원) Immunostain buffer
NZ526173A (en) * 2000-11-06 2005-03-24 Invitrogen Corp Dry powder cells and cell culture reagents and methods of production thereof
AU2002224894A1 (en) * 2000-12-21 2002-07-01 Merck Patent G.M.B.H Method for generating competent or transformed cells
US20020114795A1 (en) 2000-12-22 2002-08-22 Thorne Kevin J. Composition and process for bone growth and repair
US6682695B2 (en) 2001-03-23 2004-01-27 Clearant, Inc. Methods for sterilizing biological materials by multiple rates
US6696060B2 (en) * 2001-06-14 2004-02-24 Clearant, Inc. Methods for sterilizing preparations of monoclonal immunoglobulins
US6946098B2 (en) 2001-08-10 2005-09-20 Clearant, Inc. Methods for sterilizing biological materials
US20030031584A1 (en) * 2001-08-10 2003-02-13 Wilson Burgess Methods for sterilizing biological materials using dipeptide stabilizers
US7252799B2 (en) 2001-08-31 2007-08-07 Clearant, Inc. Methods for sterilizing preparations containing albumin
US6749851B2 (en) 2001-08-31 2004-06-15 Clearant, Inc. Methods for sterilizing preparations of digestive enzymes
US20030091971A1 (en) * 2001-09-14 2003-05-15 Invitrogen Corporation Composition for stabilizing biological materials
US20110091353A1 (en) * 2001-09-24 2011-04-21 Wilson Burgess Methods for Sterilizing Tissue
US20030095890A1 (en) * 2001-09-24 2003-05-22 Shirley Miekka Methods for sterilizing biological materials containing non-aqueous solvents
US20030185702A1 (en) * 2002-02-01 2003-10-02 Wilson Burgess Methods for sterilizing tissue
US6783968B2 (en) 2001-09-24 2004-08-31 Clearant, Inc. Methods for sterilizing preparations of glycosidases
US20050014201A1 (en) * 2001-10-25 2005-01-20 Mordechai Deuthsch Interactive transparent individual cells biochip processor
JP4837892B2 (en) * 2001-11-01 2011-12-14 ネクター セラピューティクス Method for producing powder batch
EP2253699A1 (en) * 2001-11-30 2010-11-24 Life Technologies Corporation Dry powder cell culture products containing lipid and methods of production thereof
US20030124023A1 (en) * 2001-12-21 2003-07-03 Wilson Burgess Method of sterilizing heart valves
US20030180181A1 (en) * 2002-02-01 2003-09-25 Teri Greib Methods for sterilizing tissue
GB0216562D0 (en) * 2002-04-25 2002-08-28 Bradford Particle Design Ltd Particulate materials
US6908591B2 (en) * 2002-07-18 2005-06-21 Clearant, Inc. Methods for sterilizing biological materials by irradiation over a temperature gradient
DE10234165B4 (en) * 2002-07-26 2008-01-03 Advanced Micro Devices, Inc., Sunnyvale A method of filling a trench formed in a substrate with an insulating material
AU2003302329B2 (en) * 2002-12-30 2010-01-07 Novartis Ag Prefilming atomizer
US7033612B2 (en) * 2003-01-03 2006-04-25 Kang David S Composition and method for treating age-related disorders
IL154677A0 (en) * 2003-02-27 2003-09-17 Univ Bar Ilan A method and apparatus for manipulating an individual cell
EP1656443A4 (en) * 2003-05-02 2007-06-06 Sigma Aldrich Co Solid phase cell lysis and capture platform
US9200245B2 (en) 2003-06-26 2015-12-01 Seng Enterprises Ltd. Multiwell plate
US7888110B2 (en) * 2003-06-26 2011-02-15 Seng Enterprises Ltd. Pico liter well holding device and method of making the same
US20060240548A1 (en) * 2003-06-26 2006-10-26 Mordechai Deutsch Materials for constructing cell-chips, cell-chip covers, cell-chips coats, processed cell-chips and uses thereof
US8597597B2 (en) * 2003-06-26 2013-12-03 Seng Enterprises Ltd. Picoliter well holding device and method of making the same
WO2005008626A1 (en) * 2003-07-11 2005-01-27 University Of North Carolina At Chapel Hill Methods and systems for controlling a computer using a video image and for combining the video image with a computer desktop
US20050064524A1 (en) * 2003-08-11 2005-03-24 Mordechai Deutsch Population of cells utilizable for substance detection and methods and devices using same
AU2005210236B2 (en) * 2004-02-03 2010-09-09 Chemagis Ltd. Stable amorphous forms of montelukast sodium
EP1763665A1 (en) * 2004-07-07 2007-03-21 Seng Enterprises Limited Method and device for identifying an image of a well in an image of a well-bearing component
US7403647B2 (en) * 2004-09-13 2008-07-22 Seng Enterprises Ltd. Method for identifying an image of a well in an image of a well-bearing component
WO2006021959A2 (en) * 2004-08-25 2006-03-02 Seng Enterprises Ltd. Method and device for isolating cells
WO2006026248A1 (en) * 2004-08-25 2006-03-09 Sigma-Aldrich Co. Compositions and methods employing zwitterionic detergent combinations
DE602005023534D1 (en) 2005-01-25 2010-10-21 Seng Entpr Ltd MICROFLUIDIC DEVICE FOR INVESTIGATING CELLS
DE102005034043B4 (en) * 2005-07-18 2019-12-12 Südzucker Aktiengesellschaft Mannheim/Ochsenfurt Mixture containing L-carnitine and trehalulose and product containing the mixture
US20070141555A1 (en) * 2005-10-11 2007-06-21 Mordechai Deutsch Current damper for the study of cells
US8288120B2 (en) * 2005-11-03 2012-10-16 Seng Enterprises Ltd. Method for studying floating, living cells
US20060223999A1 (en) * 2006-05-10 2006-10-05 Chemagis Ltd. Process for preparing montelukast and precursors thereof
US7718616B2 (en) 2006-12-21 2010-05-18 Zimmer Orthobiologics, Inc. Bone growth particles and osteoinductive composition thereof
US9975118B2 (en) 2007-11-15 2018-05-22 Seng Enterprises Ltd. Device for the study of living cells
US9145540B1 (en) 2007-11-15 2015-09-29 Seng Enterprises Ltd. Device for the study of living cells
US9867782B2 (en) * 2009-04-09 2018-01-16 Entegrion, Inc. Spray-dried blood products and methods of making same
US20120003188A1 (en) * 2010-05-28 2012-01-05 Garnet Biotherapeutics, Inc. Compositions and Methods of Using Living and Non-Living Bioactive Devices with Components Derived from Self-Renewing Colony Forming Cells Cultured and Expanded In Vitro
AU2011329054B2 (en) 2010-11-15 2015-05-28 Zimmer Orthobiologics, Inc. Bone void fillers
KR101899175B1 (en) 2010-12-16 2018-09-14 메르크 파텐트 게엠베하 Dry granulated cell culture media
CN107699540A (en) 2011-02-07 2018-02-16 生命技术公司 For stablizing the composition and method of sensitive compound
HUE034396T2 (en) * 2011-07-12 2018-02-28 Foodchek Systems Inc Culture medium, method for culturing salmonella and e. coli and method for detecting salmonella and e. coli
CN104080905B (en) 2011-12-22 2016-11-16 生命技术公司 cell culture medium and method
EP2674482A1 (en) * 2012-06-15 2013-12-18 Merck Patent GmbH Process for producing cell culture media
JP6388579B2 (en) * 2012-07-25 2018-09-12 スリーエム イノベイティブ プロパティズ カンパニー Aggregating microorganism medium
KR102058491B1 (en) * 2012-08-07 2019-12-23 쓰리엠 이노베이티브 프로퍼티즈 컴파니 Method of making agglomerated microbiological media and compositions thereof
JP2016526391A (en) * 2013-07-11 2016-09-05 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツングMerck Patent Gesellschaft mit beschraenkter Haftung Cell culture media
RU2681288C2 (en) * 2014-02-21 2019-03-05 Лайф Текнолоджиз Корпорейшн Systems, methods and apparatuses for media rehydration
MX2016013223A (en) * 2014-04-10 2017-04-27 Bayer Healthcare Llc Compounded media powder formulation and method of preparation of liquid medium for cell culture.
KR101617858B1 (en) * 2014-07-07 2016-05-04 유한책임회사 암브로티아 Wet Granularized Cell Culture Medium and Method for Producing the Same
US20180223250A1 (en) * 2015-08-05 2018-08-09 Merck Patent Gmbh Process for producing cell culture media
RU2021132093A (en) * 2015-12-17 2021-12-07 Лайф Текнолоджиз Корпорейшн GRANULES USED IN CELL CULTURE AND METHODS FOR THEIR PRODUCTION
EP4008772A1 (en) * 2015-12-30 2022-06-08 Life Technologies Corporation Layered cell culture particles and methods of making thereof
MA45453A (en) * 2016-06-24 2019-05-01 Agbiome Inc PROCESSES AND COMPOSITIONS FOR THE SPRAY DRYING OF GRAM NEGATIVE BACTERIA
WO2018019812A1 (en) 2016-07-28 2018-02-01 Merck Patent Gmbh Process for improving the dissolution behaviour of components in aqueous solutions
US20200008299A1 (en) * 2016-10-21 2020-01-02 Bao Tran Flexible printed electronics
EP3875595A4 (en) * 2018-11-02 2022-07-13 Kyowa Kirin Co., Ltd. Liquid medium preparation method
KR20220062409A (en) 2019-09-19 2022-05-16 라이프 테크놀로지스 코포레이션 Cell Culture Media Tablets and Methods of Preparation
WO2021094341A1 (en) 2019-11-14 2021-05-20 Merck Patent Gmbh Cell culture media
JP2023507421A (en) 2019-12-19 2023-02-22 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング Methods and compositions for modulating the glycosylation profile of proteins
JP2023531963A (en) 2020-06-22 2023-07-26 ライフ テクノロジーズ コーポレーション Methods and compositions for culturing pluripotent cell suspensions
JP2023546222A (en) 2020-10-21 2023-11-01 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング Muscle targeting complex and its use to treat myotonic dystrophy
EP4261274A1 (en) 2022-04-13 2023-10-18 Merck Patent GmbH Meroxapols for cell culture

Family Cites Families (93)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1355192U (en)
US2835586A (en) 1953-07-27 1958-05-20 Instant Milk Company Dried milk product and method of making same
NL6601640A (en) * 1965-02-11 1966-08-12
DE1949690A1 (en) 1969-10-02 1971-04-08 Egon Laumen Plastics foil container for granular or - liquid materials esp milk
DK123067B (en) * 1970-09-25 1972-05-15 Niro Atomizer As Process for treating a powdered, fatty milk product.
GB1355192A (en) * 1970-12-21 1974-06-05 Niro Atomizer As Methods for producing agglomerated coffee powder and other powders and apparatus for carrying out the method
DK125813B (en) 1971-04-16 1973-05-07 Niro Atomizer As Apparatus for drying moist powders.
US4072570A (en) * 1972-04-11 1978-02-07 Beatrice Foods Co. Preparation of a tuberculosis test medium by reconstituting a storage stabilized dry powdered Lowenstein-Jensen medium
CS169007B1 (en) 1973-05-18 1976-06-29
US4073951A (en) * 1974-04-18 1978-02-14 Sargeant Ralph G Agglomeration method
IL44853A (en) 1974-05-17 1975-11-25 Isorad Isotopes & Radiation Process for the preparation of sterile culture media and culture medium thus obtained
US3920857A (en) * 1974-10-21 1975-11-18 Central Soya Co Method of preparing a granular free-flowing lecithin product
JPS5652542B2 (en) 1975-02-24 1981-12-12
DD119304A1 (en) 1975-04-23 1976-04-12
US4053542A (en) 1976-07-22 1977-10-11 Acf Industries, Inc. Control means for secondary throttle
US4282326A (en) * 1978-10-12 1981-08-04 Jeanne Moldenhauer Cell culture medium supplement
US4241186A (en) * 1978-12-18 1980-12-23 Conviron, Inc. Pectin culture media and method
US4615978A (en) 1979-06-28 1986-10-07 The State Of Oregon, By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University Bacterial growth medium and method of use
US4282255A (en) * 1979-06-28 1981-08-04 State Of Oregon, By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University Method and starter compositions for the growth of acid producing bacteria and bacterial compositions produced thereby
DE3171242D1 (en) 1980-10-06 1985-08-08 Chlorella Industry Co Cell culture method
JPS57152882A (en) 1981-03-14 1982-09-21 Kikujiro Ishiwatari Method for killing living general chlorella
JPS57206398A (en) 1981-06-15 1982-12-17 Eiken Kagaku Kk Dried culture medium for identifying cell
US4416898A (en) * 1982-03-01 1983-11-22 Pharmuka Laboratoires Therapeutic uses of methionine
JPH07105474B2 (en) 1983-09-28 1995-11-13 株式会社日立製作所 Semiconductor memory
US4511592A (en) 1982-03-11 1985-04-16 Scm Corporation Preparation of acidulated meat emulsions
DK157053B (en) * 1982-06-14 1989-11-06 Niro Atomizer As PROCEDURE FOR THE PREPARATION OF AN AGGLOMERATED POWDER-MILK PRODUCT
US4544637A (en) 1982-09-14 1985-10-01 Igi Biotechnology, Inc. Culture media from clarified diary whey lactose permeates
DD228951A3 (en) * 1982-12-02 1985-10-23 Inst Getreideverarbeitung PROCESS FOR PRODUCING GRANULATED FOOD
US4511593A (en) * 1983-01-17 1985-04-16 Multi-Arc Vacuum Systems Inc. Vapor deposition apparatus and method
SU1227145A1 (en) 1983-02-25 1986-04-30 Опытное Контрукторско-Технологическое Бюро По Интенсификации Тепломассообменных Процессов Института Технической Теплофизики Ан Усср Method of producing acidophilous preparation
JPS59501933A (en) 1983-03-02 1984-11-22 アイジ−アイ、バイオテクノロジ−、インコ−ポレイテッド Conversion of clarified dairy whey lactose permeate into media and other commercially useful products
US4621058A (en) 1983-04-11 1986-11-04 Mid-America Dairymen, Inc. Method of preparing cheese starter media
US4673649A (en) 1983-07-15 1987-06-16 University Patents, Inc. Process and defined medium for growth of human epidermal keratinocyte cells
US4620908A (en) 1983-10-03 1986-11-04 Biocell Laboratories, Inc. Method for destroying microbial contamination in protein materials
FR2559499B1 (en) 1984-02-09 1986-09-19 Hoechst Behring Applic Pharmac NOVEL, SOLID, FAST-DELIVERY CULTURE MEDIUM, READY TO USE AND PROCESS FOR PREPARING THE SAME
US4689297A (en) 1985-03-05 1987-08-25 Miles Laboratories, Inc. Dust free particulate enzyme formulation
US4632980A (en) * 1985-04-03 1986-12-30 Immunologics Ozone decontamination of blood and blood products
US4975246A (en) 1985-09-30 1990-12-04 Charm Stanley E High temperature, short time heating system and method of heating heat-sensitive material
US4820627A (en) * 1986-03-24 1989-04-11 Em Diagnostic Systems, Inc. Method of preparing particles suitable for tabletting into diagnostic reagents
GB8620003D0 (en) 1986-08-16 1986-09-24 Arbuthnoit R D Accessory device
JPH067791B2 (en) 1986-12-12 1994-02-02 農薬バイオテクノロジ−開発技術研究組合 Method for culturing insect cells in autoclave sterilized serum-free medium
GB8700354D0 (en) 1987-01-08 1987-02-11 Distillers Co Culture of micro-organisms
EP0283942B1 (en) 1987-03-24 1992-05-20 W.R. Grace & Co.-Conn. Basal nutrient medium for cell culture
DK158532C (en) 1987-07-14 1990-10-29 Niro Atomizer As TREATMENT DEVICE WORKING WITH FLUID BED AND RENT PLATE
US4848122A (en) * 1987-07-20 1989-07-18 Chemtronics Method and apparatus for deburring using shot
GB8718756D0 (en) * 1987-08-07 1987-09-16 Unilever Plc Supporting means
JPH0646941B2 (en) * 1987-12-11 1994-06-22 サントール株式会社 Microbial culture method
GB8800572D0 (en) 1988-01-12 1988-02-10 Red Baron Oil Tools Rental Improvements in/relating to drilling tool
US5006204A (en) 1988-08-10 1991-04-09 A/S Niro Atomizer Apparatus for crystallizing whey
JPH0257175A (en) * 1988-08-19 1990-02-26 Morinaga & Co Ltd Formed culture medium and production thereof
NO901662L (en) * 1989-04-17 1990-12-21 Monsanto Co DRY HERBICID PREPARATION WITH IMPROVED WATER SOLUBILITY.
EP0441092A1 (en) 1990-02-08 1991-08-14 Niro Holding A/S A method and apparatus for heat treating a particulate product
US5254073A (en) * 1990-04-27 1993-10-19 Kapak Corporation Method of making a vented pouch
DK0474949T3 (en) 1990-09-11 1995-01-30 Niro Holding As Method and apparatus for treating a powdered or particulate material or product with gas
JPH0761259B2 (en) 1990-10-08 1995-07-05 工業技術院長 Extraction method of yeast intracellular substance
JPH04198137A (en) 1990-11-28 1992-07-17 Sumitomo Pharmaceut Co Ltd Production of dried sterile sodium carbonate
JP2895641B2 (en) * 1991-02-28 1999-05-24 ハウス食品株式会社 Animal cell growth promoter and animal cell growth method using the same
US5700426A (en) 1991-03-08 1997-12-23 Foundation Nationale De Transfusion Sanguine Method for decontaminating or sterilizing "in situ" a vacuum sealed container and device for implementing such method
DK0507038T3 (en) 1991-04-05 1994-10-17 Niro Holding As Apparatus with fluidizing bed and method for making same
GB9107628D0 (en) 1991-04-10 1991-05-29 Moonbrook Limited Preparation of diagnostic agents
DE69231331T2 (en) 1991-06-17 2001-02-22 Life Technologies Inc TECHNOLOGY FOR CONCENTRATING CULTURAL MEDIA
US5155039A (en) 1991-07-22 1992-10-13 Chrisope Technologies, Inc. Apparatus for methods for preserving, transporting storing, re-hydrating and delivering viable micro-organisms
US5445792A (en) * 1992-03-13 1995-08-29 American Sterilizer Company Optimum hydrogen peroxide vapor sterlization method
US5325606A (en) 1992-12-23 1994-07-05 Niro A/S Process and apparatus for drying and calcining sodium bicarbonate
US5366696A (en) 1993-01-07 1994-11-22 1077075 Ontario Inc. Oxygenation apparatus for oxygenating a carrier liquid by spraying
WO1994027645A1 (en) * 1993-05-28 1994-12-08 Porton International Plc System for packaging and delivering of a sterile powder medium
BR9406778A (en) 1993-06-15 1996-03-19 Commw Scient Ind Res Org Fumigation process and apparatus
DK75293D0 (en) * 1993-06-24 1993-06-24 Anhydro As PROCEDURE AND PLANT FOR AN AGGLOMERATED PRODUCT
US5631159A (en) 1993-09-22 1997-05-20 Creative Biomolecules, Inc. Lipid-modified serum free media
DK62994A (en) * 1993-11-15 1995-05-16 Niro Holding As Apparatus and method for making an agglomerated material
DE69312331T2 (en) 1993-11-15 1998-02-19 Niro Holding As FLUID BED FOR PRODUCING AND / OR TREATING A PARTICULATE MATERIAL
US5563068A (en) * 1994-04-21 1996-10-08 Genetic Therapy, Inc. Bioreactor
US5605889A (en) * 1994-04-29 1997-02-25 Pfizer Inc. Method of administering azithromycin
US5580856A (en) 1994-07-15 1996-12-03 Prestrelski; Steven J. Formulation of a reconstituted protein, and method and kit for the production thereof
US5662897A (en) * 1994-07-27 1997-09-02 U. Of Ga Research Foundation Insect viruses, sequences, insecticidal compositions and methods of use
US5733774A (en) * 1995-02-02 1998-03-31 Ecoscience Corporation Method and composition for producing stable bacteria and bacterial formulations
US5811406A (en) 1995-06-07 1998-09-22 Regents Of The University Of California Dry powder formulations of polynucleotide complexes
JPH11513248A (en) * 1995-09-18 1999-11-16 ミネソタ・マイニング・アンド・マニュファクチャリング・カンパニー Thin-film culture plate device containing granular medium particles
DE19535581C1 (en) * 1995-09-25 1996-10-31 Cpc Maizena Gmbh Coagulated, flavoured egg cubes for adding e.g. to hot soup
CA2235069C (en) 1995-10-19 2010-12-14 Advanced Reproduction Technologies, Inc. Methods and compositions to improve germ cell and embryo survival and function
SE507856C2 (en) 1996-04-12 1998-07-20 Acanova Ab Heat remediation of seeds
US6632648B1 (en) 1996-05-14 2003-10-14 Elan Drug Delivery Limited Methods of terminal sterilization of fibrinogen
US5773279A (en) 1996-11-27 1998-06-30 Neogen Corporation Culture medium and method for repair of microbial cells
US6383810B2 (en) 1997-02-14 2002-05-07 Invitrogen Corporation Dry powder cells and cell culture reagents and methods of production thereof
US20060003448A1 (en) * 2003-12-30 2006-01-05 Richard Fike Dry powder cells and cell culture reagents and methods of production thereof
US20040022666A1 (en) * 1998-06-30 2004-02-05 Invitrogen Corporation Methods for reducing adventitious agents and toxins and cell culture reagents produced thereby
US20060003447A1 (en) * 2003-12-30 2006-01-05 Richard Fike Dry powder cells and cell culture reagents and methods of production thereof
US6627426B2 (en) 1997-02-14 2003-09-30 Invitrogen Corporation Methods for reducing adventitious agents and toxins and cell culture reagents produced thereby
US6210715B1 (en) * 1997-04-01 2001-04-03 Cap Biotechnology, Inc. Calcium phosphate microcarriers and microspheres
US6045838A (en) * 1998-08-10 2000-04-04 Davis; Harold L. Grape handling and storage bag
US6206571B1 (en) 1999-06-24 2001-03-27 Alan D. Olin Flexible bag with resealable pour spout
NZ526173A (en) * 2000-11-06 2005-03-24 Invitrogen Corp Dry powder cells and cell culture reagents and methods of production thereof
EP2253699A1 (en) * 2001-11-30 2010-11-24 Life Technologies Corporation Dry powder cell culture products containing lipid and methods of production thereof

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