CA2280741A1 - Dry powder cells and cell culture reagents and methods of production thereof - Google Patents
Dry powder cells and cell culture reagents and methods of production thereof Download PDFInfo
- Publication number
- CA2280741A1 CA2280741A1 CA002280741A CA2280741A CA2280741A1 CA 2280741 A1 CA2280741 A1 CA 2280741A1 CA 002280741 A CA002280741 A CA 002280741A CA 2280741 A CA2280741 A CA 2280741A CA 2280741 A1 CA2280741 A1 CA 2280741A1
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- powder
- cell
- nutritive medium
- supplement
- serum
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0025—Culture media for plant cell or plant tissue culture
Abstract
The present invention relates generally to nutritive medium, medi0m supplement, media subgroup and buffer formulations. Specifically, the present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention is particularly directed to methods of production of these media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations. The invention also relates to methods of producing sterile powdered media, media supplement (particularly powdered sera such as powdered FBS, powdered transferrin, powdered insulin, powdered organ extracts (such as bovine brain or pituitary extracts), powdered growth factors (such as EGF, FGF, etc.) and the like), media subgroup and buffer formulations. In a particularly preferred aspect, the invention relates to such methods wherein the sterilization is accomplished by gamma irradiation.
The invention also relates to methods for producing dry cell powders, comprising spray-drying a cell suspension. The invention also relates to cell, media, media supplement, media subgroup and buffer powders produced by these methods.
The invention also relates to methods for producing dry cell powders, comprising spray-drying a cell suspension. The invention also relates to cell, media, media supplement, media subgroup and buffer powders produced by these methods.
Claims (91)
1. A method of producing a nutritive medium powder, said method comprising agglomerating a dry powder nutritive medium with a solvent.
2. A method of producing a nutritive medium supplement powder, said method comprising agglomerating a dry powder nutritive medium supplement with a solvent.
3 . A method of producing a nutritive medium subgroup powder, said method comprising agglomerating a dry powder nutritive medium subgroup with a solvent.
4. A method of producing a buffer powder, said method comprising agglomerating a dry powder buffer with a solvent.
5. A method of producing a nutritive medium powder, said method comprising spray-drying a liquid nutritive medium.
6. A method of producing a nutritive medium supplement powder, said method comprising spray-drying a liquid nutritive medium supplement.
7. A method of producing a nutritive medium subgroup powder, said method comprising spray-drying a liquid nutritive medium subgroup.
8. A method of producing a buffer powder, said method comprising spray-drying a liquid buffer solution.
9. The method of any one of claims 5-8, further comprising agglomerating said powder with a solvent.
10. The method of any one of claims 5-8, wherein said spray-drying is performed under conditions of controlled temperature and humidity.
11. The method of any one of claims 1-8, further comprising packaging said powder.
12. The method of any one of claims 1-8, further comprising sterilizing said powder.
13. The method of claim 12, wherein said sterilization is performed after packaging said powder.
14. The method of claim 12, wherein said sterilization is accomplished by irradiation of said powder with gamma rays until said powder is rendered substantially sterile.
15. The method of claim 1 or claim 5, further comprising adding at least one medium supplement to said agglomerated nutritive medium by spray-drying said supplement onto said agglomerated medium.
16. The method of claim 1 or claim 5, further comprising adding an acid or base to said nutritive medium by agglomerating said acid or base into said nutritive medium.
17. The method of claim 1 or claim 5, wherein said nutritive medium powder is a cell culture medium powder selected from the group consisting of a bacterial cell culture medium powder, a yeast cell culture medium powder, a plant cell culture medium powder and an animal cell culture medium powder.
18. The method of claim 1 or claim 5, wherein said nutritive medium comprises one or more ingredients selected from the group consisting of serum, L-glutamine, insulin, transferrin, a lipid, a cytokine, a neurotransmitter and a buffer.
19. The method of claim 2, wherein said nutritive medium supplement is serum.
20. The method of claim 3, wherein said nutritive medium supplement is serum.
21. The method of claim 15, wherein said nutritive medium supplement is serum.
22. The method of any one of claims 19-21, wherein said serum is bovine serum or human serum.
23. The method of claim 22, wherein said bovine serum is fetal bovine serum or calf serum.
24. The method of claim 18, wherein said buffer is sodium bicarbonate.
25. The method of claim 4 or claim 8, wherein said buffer powder is a buffered saline powder.
26. The method of claim 25, wherein said buffered saline powder is a phosphate-buffered saline powder or a Tris-buffered saline powder.
27. A nutritive medium powder prepared according to the method of claim 1.
28. A nutritive medium powder prepared according to the method of claim 5 .
29. A nutritive medium powder prepared according to the method of claim 9.
30. A nutritive medium powder prepared according to the method of claim 16.
31. An automatically pH-adjusting nutritive medium powder.
32. The nutritive medium powder of claim 31, wherein said powder comprises at least one buffer salt.
33. The nutritive medium powder of claim 32, wherein said buffer salt is sodium bicarbonate.
34. The nutritive medium powder of any one of claims 27-31, wherein said nutritive medium powder comprises at least one buffer salt.
35. The nutritive medium powder of claim 34, wherein said buffer salt is sodium bicarbonate.
36. The nutritive medium powder of any one of claims 27-31, wherein said nutritive medium has a pH of between 7.1-7.5 when said medium is reconstituted with a solvent.
37. A nutritive medium supplement powder prepared according to the method of claim 2.
38. A nutritive medium supplement powder prepared according to the method of claim 3.
39. A nutritive medium subgroup powder prepared according to the method of claim 3.
40. A nutritive medium subgroup powder prepared according to the method of claim 7.
41. A nutritive medium subgroup powder prepared according to the method of claim 9.
42. A buffer powder prepared according to the method of claim 4
43. A buffer powder prepared according to the method of claim 8.
44. The nutritive medium supplement powder of claim 37 or claim 38, wherein said nutritive medium supplement is serum.
45. The nutritive medium supplement powder of claim 44, wherein said serum is bovine serum or human serum.
46. The nutritive medium supplement powder of claim 45, wherein said bovine serum is fetal bovine serum or calf serum.
47. The nutritive medium supplement powder of claim 37 or claim 38, wherein said nutritive medium supplement powder comprises one or more nutritive medium components.
48. A method of culturing a cell comprising reconstituting the nutritive medium powder of any one of claims 27-31 with a solvent to form a liquid solution and contacting the cell with said liquid solution under conditions favoring the cultivation of the cell.
49. The method of claim 48, wherein said solvent comprises serum or water.
50. The method of claim 48, wherein said cell is selected from the group consisting of a bacterial cell, a yeast cell, a plant cell and an animal cell.
51. The method of claim 50, wherein said animal cell is an insect cell, a nematode cell or a mammalian cell.
52. The method of claim 51, wherein said mammalian cell is selected from the group consisting of a CHO cell, a COS cell, a VERO cell, a BHK cell, an AE-1 cell, an SP2/0 cell, an L5.1 cell, a hybridoma cell, and a human cell.
53. The method of claim 48, wherein said cell is an anchorage-dependent cell.
54. The method of claim 48, wherein said cell is an anchorage-independent cell.
55. The method of claim 48 wherein said cell is selected from the group consisting of a normal cell, a diseased cell, a transformed cell, a mutant cell, a somatic cell, a germ cell, a stem cell, a precursor cell and an embryonic cell.
56. A method of producing a sterile nutritive medium supplement powder, comprising:
(a) obtaining a nutritive medium supplement powder to be sterilized; and (b) sterilizing said powder by irradiation of said powder with y rays until said powder is rendered sterile.
(a) obtaining a nutritive medium supplement powder to be sterilized; and (b) sterilizing said powder by irradiation of said powder with y rays until said powder is rendered sterile.
57. The method of claim 56, wherein said nutritive medium supplement powder is prepared by lyophilizing a liquid nutritive medium supplement.
58. The method of claim 56, wherein said nutritive medium supplement powder is prepared by spray-drying a liquid nutritive medium supplement.
59. The method of claim 56, wherein said nutritive medium supplement powder is prepared by agglomerating a powdered nutritive medium supplement.
60. A sterile nutritive medium supplement powder prepared according to the method of claim 56.
61. The sterile nutritive medium supplement powder of claim 60, wherein said powder is selected from the group consisting of a serum powder, a cytokine powder, an insulin powder, a lipid powder, an animal organ extract powder, an animal gland extract powder, an animal tissue extract powder, an enzyme powder and a transferrin powder.
62. The sterile nutritive medium supplement powder of claim 60, wherein said powder is a serum powder.
63. The sterile nutritive medium supplement powder of claim 61 or claim 62, wherein said serum powder is a bovine serum powder or a human serum powder.
64. The sterile nutritive medium supplement powder of claim 63, wherein said bovine serum powder is a fetal bovine serum powder or a calf serum powder.
65. The sterile nutritive medium supplement powder of claim 61, wherein said cytokine powder is a growth factor powder, an interleukin powder, a colony-stimulating factor powder or an interferon powder.
66. The sterile nutritive medium supplement powder of claim 61, wherein said lipid powder is a phospholipid powder, a sphingolipid powder, a fatty acid powder or a cholesterol powder.
67. The sterile nutritive medium supplement powder of claim 61, wherein said animal gland extract powder is a bovine pituitary extract powder.
68. The sterile nutritive medium supplement powder of claim 61, wherein said enzyme powder is a trypsin powder, a collagenase powder, a pancreatinin powder or a dispase powder.
69. The sterile nutritive medium supplement powder of claim 60, wherein said powder is a transferrin powder.
70. A kit for use in the cultivation of a cell, said kit comprising one or more containers wherein a first container contains the powder of any one of claims 27-31, 37-43 or 60.
71. The kit of claim 70, further comprising one or more additional containers containing one or more cells.
72. The kit of claim 70, further comprising one or more additional containers containing one or more solvents.
73. A method of producing a dry cell powder comprising at least one cell, said method comprising:
(a) obtaining a cell to be dried;
(b) forming a cell suspension by suspending said cell in an aqueous solution; and (c) producing a powder by spray-drying said cell suspension.
(a) obtaining a cell to be dried;
(b) forming a cell suspension by suspending said cell in an aqueous solution; and (c) producing a powder by spray-drying said cell suspension.
74. The method of claim 73, further comprising contacting said cell with at least one stabilizing or preserving compound.
75. The method of claim 74, wherein said stabilizing or preserving compound is a polysaccharide.
76. The method of claim 75, wherein said polysaccharide is trehalose.
77. The method of claim 74, wherein said cell is contacted with the stabilizing or preserving compound by spray-drying or agglomerating the compound onto said dry cell powder.
78. The method of claim 73, wherein said aqueous solution comprises one or more components selected from the group consisting of a buffer, a salt, a nutritive medium component and a nutritive medium supplement.
79. The method of claim 73, further comprising agglomerating said dry cell powder with a solvent.
80. The method of claim 79, wherein said solvent is selected from the group consisting of a buffer solution, a salt solution, a nutritive medium supplement solution, a nutritive medium solution, and a combination thereof.
81. The method of claim 78 or claim 80, wherein said nutritive medium supplement is serum.
82. The method of claim 81, wherein said serum is bovine serum or human serum.
83. The method of claim 82, wherein said bovine serum is fetal bovine serum or calf serum.
84. The method of claim 73, wherein said cell is a bacterial cell, a yeast cell, an animal cell or a plant cell.
85. The method of claim 84, wherein said animal cell is an insect cell, a nematode cell or a mammalian cell.
86. The method of claim 85, wherein said mammalian cell is a human cell.
87. The method of claim 85, wherein said mammalian cell is a non-human cell.
88. The method of claim 73, wherein said cell is selected from the group consisting of a normal cell, a diseased cell, a transformed cell, a mutant cell, a somatic cell, a germ cell, a stem cell, a precursor cell and an embryonic cell.
89. The method of claim 73, wherein said cell is an anchorage-dependent cell.
90. The method of claim 73, wherein said cell is an anchorage-independent cell.
91. A dry cell powder prepared according to the method of claim 73.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA2798535A CA2798535C (en) | 1997-02-14 | 1998-02-17 | Dry powder cells and cell culture reagents and methods of production thereof |
Applications Claiming Priority (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US4031497P | 1997-02-14 | 1997-02-14 | |
US60/040,314 | 1997-02-14 | ||
US5871697P | 1997-09-12 | 1997-09-12 | |
US60/058,716 | 1997-09-12 | ||
US6219297P | 1997-10-16 | 1997-10-16 | |
US60/062,192 | 1997-10-16 | ||
PCT/US1998/002829 WO1998036051A1 (en) | 1997-02-14 | 1998-02-17 | Dry powder cells and cell culture reagents and methods of production thereof |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2798535A Division CA2798535C (en) | 1997-02-14 | 1998-02-17 | Dry powder cells and cell culture reagents and methods of production thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2280741A1 true CA2280741A1 (en) | 1998-08-20 |
CA2280741C CA2280741C (en) | 2013-04-09 |
Family
ID=27365699
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2798535A Expired - Lifetime CA2798535C (en) | 1997-02-14 | 1998-02-17 | Dry powder cells and cell culture reagents and methods of production thereof |
CA2280741A Expired - Lifetime CA2280741C (en) | 1997-02-14 | 1998-02-17 | Dry powder cells and cell culture reagents and methods of production thereof |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2798535A Expired - Lifetime CA2798535C (en) | 1997-02-14 | 1998-02-17 | Dry powder cells and cell culture reagents and methods of production thereof |
Country Status (10)
Country | Link |
---|---|
US (4) | US6383810B2 (en) |
EP (4) | EP1012234A4 (en) |
JP (8) | JP4557311B2 (en) |
AT (1) | ATE507283T1 (en) |
AU (1) | AU6654998A (en) |
CA (2) | CA2798535C (en) |
DE (1) | DE69842243D1 (en) |
DK (3) | DK2281873T3 (en) |
ES (1) | ES2516790T3 (en) |
WO (1) | WO1998036051A1 (en) |
Families Citing this family (91)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5362442A (en) * | 1993-07-22 | 1994-11-08 | 2920913 Canada Inc. | Method for sterilizing products with gamma radiation |
US20040067157A1 (en) * | 1993-07-22 | 2004-04-08 | Clearant, Inc. | Methods for sterilizing biological materials |
US6051256A (en) * | 1994-03-07 | 2000-04-18 | Inhale Therapeutic Systems | Dispersible macromolecule compositions and methods for their preparation and use |
EP0891419A4 (en) * | 1996-03-12 | 2000-03-01 | Life Technologies Inc | Hematopoietic cell culture nutrient supplement |
US20030203036A1 (en) * | 2000-03-17 | 2003-10-30 | Gordon Marc S. | Systems and processes for spray drying hydrophobic drugs with hydrophilic excipients |
JP2001512310A (en) | 1997-02-12 | 2001-08-21 | ライフ テクノロジーズ,インコーポレイテッド | Method for freeze-drying competent cells |
US6383810B2 (en) * | 1997-02-14 | 2002-05-07 | Invitrogen Corporation | Dry powder cells and cell culture reagents and methods of production thereof |
US20060003447A1 (en) * | 2003-12-30 | 2006-01-05 | Richard Fike | Dry powder cells and cell culture reagents and methods of production thereof |
US20060003448A1 (en) * | 2003-12-30 | 2006-01-05 | Richard Fike | Dry powder cells and cell culture reagents and methods of production thereof |
JP2002519009A (en) * | 1998-06-30 | 2002-07-02 | インビトロゲン・コーポレーション | Methods for attenuating exogenous factors and toxins and cell culture reagents produced thereby. |
WO2000011953A1 (en) * | 1998-09-01 | 2000-03-09 | Penn State Research Foundation | Method and apparatus for aseptic growth or processing of biomass |
AT409379B (en) | 1999-06-02 | 2002-07-25 | Baxter Ag | MEDIUM FOR PROTEIN- AND SERUM-FREE CELL CULTURE |
FR2804326B1 (en) * | 2000-01-27 | 2002-10-18 | Ellipse Pharmaceuticals | STERILIZATION PROCESS BY HIGH PRESSURE TREATMENT OF SENSITIVE ACTIVE INGREDIENTS |
US20040086420A1 (en) * | 2000-03-23 | 2004-05-06 | Macphee Martin J. | Methods for sterilizing serum or plasma |
EA005977B1 (en) * | 2000-03-23 | 2005-08-25 | Клиарант, Инк. | Methods for sterilizing biological materials |
IL136232A0 (en) * | 2000-05-18 | 2001-05-20 | Bar Ilan University Res Author | Measurements of enzymatic activity in a single, individual cell in population |
US7575761B2 (en) * | 2000-06-30 | 2009-08-18 | Novartis Pharma Ag | Spray drying process control of drying kinetics |
WO2002009669A2 (en) * | 2000-08-01 | 2002-02-07 | Inhale Therapeutic Systems, Inc. | Apparatus and process to produce particles having a narrow size distribution and particles made thereby |
KR100385713B1 (en) * | 2000-08-30 | 2003-05-27 | 사회복지법인삼성생명공익재단(삼성서울병원) | Immunostain buffer |
NZ526173A (en) * | 2000-11-06 | 2005-03-24 | Invitrogen Corp | Dry powder cells and cell culture reagents and methods of production thereof |
AU2002224894A1 (en) * | 2000-12-21 | 2002-07-01 | Merck Patent G.M.B.H | Method for generating competent or transformed cells |
US20020114795A1 (en) | 2000-12-22 | 2002-08-22 | Thorne Kevin J. | Composition and process for bone growth and repair |
US6682695B2 (en) | 2001-03-23 | 2004-01-27 | Clearant, Inc. | Methods for sterilizing biological materials by multiple rates |
US6696060B2 (en) * | 2001-06-14 | 2004-02-24 | Clearant, Inc. | Methods for sterilizing preparations of monoclonal immunoglobulins |
US6946098B2 (en) | 2001-08-10 | 2005-09-20 | Clearant, Inc. | Methods for sterilizing biological materials |
US20030031584A1 (en) * | 2001-08-10 | 2003-02-13 | Wilson Burgess | Methods for sterilizing biological materials using dipeptide stabilizers |
US7252799B2 (en) | 2001-08-31 | 2007-08-07 | Clearant, Inc. | Methods for sterilizing preparations containing albumin |
US6749851B2 (en) | 2001-08-31 | 2004-06-15 | Clearant, Inc. | Methods for sterilizing preparations of digestive enzymes |
US20030091971A1 (en) * | 2001-09-14 | 2003-05-15 | Invitrogen Corporation | Composition for stabilizing biological materials |
US20110091353A1 (en) * | 2001-09-24 | 2011-04-21 | Wilson Burgess | Methods for Sterilizing Tissue |
US20030095890A1 (en) * | 2001-09-24 | 2003-05-22 | Shirley Miekka | Methods for sterilizing biological materials containing non-aqueous solvents |
US20030185702A1 (en) * | 2002-02-01 | 2003-10-02 | Wilson Burgess | Methods for sterilizing tissue |
US6783968B2 (en) | 2001-09-24 | 2004-08-31 | Clearant, Inc. | Methods for sterilizing preparations of glycosidases |
US20050014201A1 (en) * | 2001-10-25 | 2005-01-20 | Mordechai Deuthsch | Interactive transparent individual cells biochip processor |
JP4837892B2 (en) * | 2001-11-01 | 2011-12-14 | ネクター セラピューティクス | Method for producing powder batch |
EP2253699A1 (en) * | 2001-11-30 | 2010-11-24 | Life Technologies Corporation | Dry powder cell culture products containing lipid and methods of production thereof |
US20030124023A1 (en) * | 2001-12-21 | 2003-07-03 | Wilson Burgess | Method of sterilizing heart valves |
US20030180181A1 (en) * | 2002-02-01 | 2003-09-25 | Teri Greib | Methods for sterilizing tissue |
GB0216562D0 (en) * | 2002-04-25 | 2002-08-28 | Bradford Particle Design Ltd | Particulate materials |
US6908591B2 (en) * | 2002-07-18 | 2005-06-21 | Clearant, Inc. | Methods for sterilizing biological materials by irradiation over a temperature gradient |
DE10234165B4 (en) * | 2002-07-26 | 2008-01-03 | Advanced Micro Devices, Inc., Sunnyvale | A method of filling a trench formed in a substrate with an insulating material |
AU2003302329B2 (en) * | 2002-12-30 | 2010-01-07 | Novartis Ag | Prefilming atomizer |
US7033612B2 (en) * | 2003-01-03 | 2006-04-25 | Kang David S | Composition and method for treating age-related disorders |
IL154677A0 (en) * | 2003-02-27 | 2003-09-17 | Univ Bar Ilan | A method and apparatus for manipulating an individual cell |
EP1656443A4 (en) * | 2003-05-02 | 2007-06-06 | Sigma Aldrich Co | Solid phase cell lysis and capture platform |
US9200245B2 (en) | 2003-06-26 | 2015-12-01 | Seng Enterprises Ltd. | Multiwell plate |
US7888110B2 (en) * | 2003-06-26 | 2011-02-15 | Seng Enterprises Ltd. | Pico liter well holding device and method of making the same |
US20060240548A1 (en) * | 2003-06-26 | 2006-10-26 | Mordechai Deutsch | Materials for constructing cell-chips, cell-chip covers, cell-chips coats, processed cell-chips and uses thereof |
US8597597B2 (en) * | 2003-06-26 | 2013-12-03 | Seng Enterprises Ltd. | Picoliter well holding device and method of making the same |
WO2005008626A1 (en) * | 2003-07-11 | 2005-01-27 | University Of North Carolina At Chapel Hill | Methods and systems for controlling a computer using a video image and for combining the video image with a computer desktop |
US20050064524A1 (en) * | 2003-08-11 | 2005-03-24 | Mordechai Deutsch | Population of cells utilizable for substance detection and methods and devices using same |
AU2005210236B2 (en) * | 2004-02-03 | 2010-09-09 | Chemagis Ltd. | Stable amorphous forms of montelukast sodium |
EP1763665A1 (en) * | 2004-07-07 | 2007-03-21 | Seng Enterprises Limited | Method and device for identifying an image of a well in an image of a well-bearing component |
US7403647B2 (en) * | 2004-09-13 | 2008-07-22 | Seng Enterprises Ltd. | Method for identifying an image of a well in an image of a well-bearing component |
WO2006021959A2 (en) * | 2004-08-25 | 2006-03-02 | Seng Enterprises Ltd. | Method and device for isolating cells |
WO2006026248A1 (en) * | 2004-08-25 | 2006-03-09 | Sigma-Aldrich Co. | Compositions and methods employing zwitterionic detergent combinations |
DE602005023534D1 (en) | 2005-01-25 | 2010-10-21 | Seng Entpr Ltd | MICROFLUIDIC DEVICE FOR INVESTIGATING CELLS |
DE102005034043B4 (en) * | 2005-07-18 | 2019-12-12 | Südzucker Aktiengesellschaft Mannheim/Ochsenfurt | Mixture containing L-carnitine and trehalulose and product containing the mixture |
US20070141555A1 (en) * | 2005-10-11 | 2007-06-21 | Mordechai Deutsch | Current damper for the study of cells |
US8288120B2 (en) * | 2005-11-03 | 2012-10-16 | Seng Enterprises Ltd. | Method for studying floating, living cells |
US20060223999A1 (en) * | 2006-05-10 | 2006-10-05 | Chemagis Ltd. | Process for preparing montelukast and precursors thereof |
US7718616B2 (en) | 2006-12-21 | 2010-05-18 | Zimmer Orthobiologics, Inc. | Bone growth particles and osteoinductive composition thereof |
US9975118B2 (en) | 2007-11-15 | 2018-05-22 | Seng Enterprises Ltd. | Device for the study of living cells |
US9145540B1 (en) | 2007-11-15 | 2015-09-29 | Seng Enterprises Ltd. | Device for the study of living cells |
US9867782B2 (en) * | 2009-04-09 | 2018-01-16 | Entegrion, Inc. | Spray-dried blood products and methods of making same |
US20120003188A1 (en) * | 2010-05-28 | 2012-01-05 | Garnet Biotherapeutics, Inc. | Compositions and Methods of Using Living and Non-Living Bioactive Devices with Components Derived from Self-Renewing Colony Forming Cells Cultured and Expanded In Vitro |
AU2011329054B2 (en) | 2010-11-15 | 2015-05-28 | Zimmer Orthobiologics, Inc. | Bone void fillers |
KR101899175B1 (en) | 2010-12-16 | 2018-09-14 | 메르크 파텐트 게엠베하 | Dry granulated cell culture media |
CN107699540A (en) | 2011-02-07 | 2018-02-16 | 生命技术公司 | For stablizing the composition and method of sensitive compound |
HUE034396T2 (en) * | 2011-07-12 | 2018-02-28 | Foodchek Systems Inc | Culture medium, method for culturing salmonella and e. coli and method for detecting salmonella and e. coli |
CN104080905B (en) | 2011-12-22 | 2016-11-16 | 生命技术公司 | cell culture medium and method |
EP2674482A1 (en) * | 2012-06-15 | 2013-12-18 | Merck Patent GmbH | Process for producing cell culture media |
JP6388579B2 (en) * | 2012-07-25 | 2018-09-12 | スリーエム イノベイティブ プロパティズ カンパニー | Aggregating microorganism medium |
KR102058491B1 (en) * | 2012-08-07 | 2019-12-23 | 쓰리엠 이노베이티브 프로퍼티즈 컴파니 | Method of making agglomerated microbiological media and compositions thereof |
JP2016526391A (en) * | 2013-07-11 | 2016-09-05 | メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツングMerck Patent Gesellschaft mit beschraenkter Haftung | Cell culture media |
RU2681288C2 (en) * | 2014-02-21 | 2019-03-05 | Лайф Текнолоджиз Корпорейшн | Systems, methods and apparatuses for media rehydration |
MX2016013223A (en) * | 2014-04-10 | 2017-04-27 | Bayer Healthcare Llc | Compounded media powder formulation and method of preparation of liquid medium for cell culture. |
KR101617858B1 (en) * | 2014-07-07 | 2016-05-04 | 유한책임회사 암브로티아 | Wet Granularized Cell Culture Medium and Method for Producing the Same |
US20180223250A1 (en) * | 2015-08-05 | 2018-08-09 | Merck Patent Gmbh | Process for producing cell culture media |
RU2021132093A (en) * | 2015-12-17 | 2021-12-07 | Лайф Текнолоджиз Корпорейшн | GRANULES USED IN CELL CULTURE AND METHODS FOR THEIR PRODUCTION |
EP4008772A1 (en) * | 2015-12-30 | 2022-06-08 | Life Technologies Corporation | Layered cell culture particles and methods of making thereof |
MA45453A (en) * | 2016-06-24 | 2019-05-01 | Agbiome Inc | PROCESSES AND COMPOSITIONS FOR THE SPRAY DRYING OF GRAM NEGATIVE BACTERIA |
WO2018019812A1 (en) | 2016-07-28 | 2018-02-01 | Merck Patent Gmbh | Process for improving the dissolution behaviour of components in aqueous solutions |
US20200008299A1 (en) * | 2016-10-21 | 2020-01-02 | Bao Tran | Flexible printed electronics |
EP3875595A4 (en) * | 2018-11-02 | 2022-07-13 | Kyowa Kirin Co., Ltd. | Liquid medium preparation method |
KR20220062409A (en) | 2019-09-19 | 2022-05-16 | 라이프 테크놀로지스 코포레이션 | Cell Culture Media Tablets and Methods of Preparation |
WO2021094341A1 (en) | 2019-11-14 | 2021-05-20 | Merck Patent Gmbh | Cell culture media |
JP2023507421A (en) | 2019-12-19 | 2023-02-22 | メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング | Methods and compositions for modulating the glycosylation profile of proteins |
JP2023531963A (en) | 2020-06-22 | 2023-07-26 | ライフ テクノロジーズ コーポレーション | Methods and compositions for culturing pluripotent cell suspensions |
JP2023546222A (en) | 2020-10-21 | 2023-11-01 | メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング | Muscle targeting complex and its use to treat myotonic dystrophy |
EP4261274A1 (en) | 2022-04-13 | 2023-10-18 | Merck Patent GmbH | Meroxapols for cell culture |
Family Cites Families (93)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1355192U (en) | ||||
US2835586A (en) | 1953-07-27 | 1958-05-20 | Instant Milk Company | Dried milk product and method of making same |
NL6601640A (en) * | 1965-02-11 | 1966-08-12 | ||
DE1949690A1 (en) | 1969-10-02 | 1971-04-08 | Egon Laumen | Plastics foil container for granular or - liquid materials esp milk |
DK123067B (en) * | 1970-09-25 | 1972-05-15 | Niro Atomizer As | Process for treating a powdered, fatty milk product. |
GB1355192A (en) * | 1970-12-21 | 1974-06-05 | Niro Atomizer As | Methods for producing agglomerated coffee powder and other powders and apparatus for carrying out the method |
DK125813B (en) | 1971-04-16 | 1973-05-07 | Niro Atomizer As | Apparatus for drying moist powders. |
US4072570A (en) * | 1972-04-11 | 1978-02-07 | Beatrice Foods Co. | Preparation of a tuberculosis test medium by reconstituting a storage stabilized dry powdered Lowenstein-Jensen medium |
CS169007B1 (en) | 1973-05-18 | 1976-06-29 | ||
US4073951A (en) * | 1974-04-18 | 1978-02-14 | Sargeant Ralph G | Agglomeration method |
IL44853A (en) | 1974-05-17 | 1975-11-25 | Isorad Isotopes & Radiation | Process for the preparation of sterile culture media and culture medium thus obtained |
US3920857A (en) * | 1974-10-21 | 1975-11-18 | Central Soya Co | Method of preparing a granular free-flowing lecithin product |
JPS5652542B2 (en) | 1975-02-24 | 1981-12-12 | ||
DD119304A1 (en) | 1975-04-23 | 1976-04-12 | ||
US4053542A (en) | 1976-07-22 | 1977-10-11 | Acf Industries, Inc. | Control means for secondary throttle |
US4282326A (en) * | 1978-10-12 | 1981-08-04 | Jeanne Moldenhauer | Cell culture medium supplement |
US4241186A (en) * | 1978-12-18 | 1980-12-23 | Conviron, Inc. | Pectin culture media and method |
US4615978A (en) | 1979-06-28 | 1986-10-07 | The State Of Oregon, By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University | Bacterial growth medium and method of use |
US4282255A (en) * | 1979-06-28 | 1981-08-04 | State Of Oregon, By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University | Method and starter compositions for the growth of acid producing bacteria and bacterial compositions produced thereby |
DE3171242D1 (en) | 1980-10-06 | 1985-08-08 | Chlorella Industry Co | Cell culture method |
JPS57152882A (en) | 1981-03-14 | 1982-09-21 | Kikujiro Ishiwatari | Method for killing living general chlorella |
JPS57206398A (en) | 1981-06-15 | 1982-12-17 | Eiken Kagaku Kk | Dried culture medium for identifying cell |
US4416898A (en) * | 1982-03-01 | 1983-11-22 | Pharmuka Laboratoires | Therapeutic uses of methionine |
JPH07105474B2 (en) | 1983-09-28 | 1995-11-13 | 株式会社日立製作所 | Semiconductor memory |
US4511592A (en) | 1982-03-11 | 1985-04-16 | Scm Corporation | Preparation of acidulated meat emulsions |
DK157053B (en) * | 1982-06-14 | 1989-11-06 | Niro Atomizer As | PROCEDURE FOR THE PREPARATION OF AN AGGLOMERATED POWDER-MILK PRODUCT |
US4544637A (en) | 1982-09-14 | 1985-10-01 | Igi Biotechnology, Inc. | Culture media from clarified diary whey lactose permeates |
DD228951A3 (en) * | 1982-12-02 | 1985-10-23 | Inst Getreideverarbeitung | PROCESS FOR PRODUCING GRANULATED FOOD |
US4511593A (en) * | 1983-01-17 | 1985-04-16 | Multi-Arc Vacuum Systems Inc. | Vapor deposition apparatus and method |
SU1227145A1 (en) | 1983-02-25 | 1986-04-30 | Опытное Контрукторско-Технологическое Бюро По Интенсификации Тепломассообменных Процессов Института Технической Теплофизики Ан Усср | Method of producing acidophilous preparation |
JPS59501933A (en) | 1983-03-02 | 1984-11-22 | アイジ−アイ、バイオテクノロジ−、インコ−ポレイテッド | Conversion of clarified dairy whey lactose permeate into media and other commercially useful products |
US4621058A (en) | 1983-04-11 | 1986-11-04 | Mid-America Dairymen, Inc. | Method of preparing cheese starter media |
US4673649A (en) | 1983-07-15 | 1987-06-16 | University Patents, Inc. | Process and defined medium for growth of human epidermal keratinocyte cells |
US4620908A (en) | 1983-10-03 | 1986-11-04 | Biocell Laboratories, Inc. | Method for destroying microbial contamination in protein materials |
FR2559499B1 (en) | 1984-02-09 | 1986-09-19 | Hoechst Behring Applic Pharmac | NOVEL, SOLID, FAST-DELIVERY CULTURE MEDIUM, READY TO USE AND PROCESS FOR PREPARING THE SAME |
US4689297A (en) | 1985-03-05 | 1987-08-25 | Miles Laboratories, Inc. | Dust free particulate enzyme formulation |
US4632980A (en) * | 1985-04-03 | 1986-12-30 | Immunologics | Ozone decontamination of blood and blood products |
US4975246A (en) | 1985-09-30 | 1990-12-04 | Charm Stanley E | High temperature, short time heating system and method of heating heat-sensitive material |
US4820627A (en) * | 1986-03-24 | 1989-04-11 | Em Diagnostic Systems, Inc. | Method of preparing particles suitable for tabletting into diagnostic reagents |
GB8620003D0 (en) | 1986-08-16 | 1986-09-24 | Arbuthnoit R D | Accessory device |
JPH067791B2 (en) | 1986-12-12 | 1994-02-02 | 農薬バイオテクノロジ−開発技術研究組合 | Method for culturing insect cells in autoclave sterilized serum-free medium |
GB8700354D0 (en) | 1987-01-08 | 1987-02-11 | Distillers Co | Culture of micro-organisms |
EP0283942B1 (en) | 1987-03-24 | 1992-05-20 | W.R. Grace & Co.-Conn. | Basal nutrient medium for cell culture |
DK158532C (en) | 1987-07-14 | 1990-10-29 | Niro Atomizer As | TREATMENT DEVICE WORKING WITH FLUID BED AND RENT PLATE |
US4848122A (en) * | 1987-07-20 | 1989-07-18 | Chemtronics | Method and apparatus for deburring using shot |
GB8718756D0 (en) * | 1987-08-07 | 1987-09-16 | Unilever Plc | Supporting means |
JPH0646941B2 (en) * | 1987-12-11 | 1994-06-22 | サントール株式会社 | Microbial culture method |
GB8800572D0 (en) | 1988-01-12 | 1988-02-10 | Red Baron Oil Tools Rental | Improvements in/relating to drilling tool |
US5006204A (en) | 1988-08-10 | 1991-04-09 | A/S Niro Atomizer | Apparatus for crystallizing whey |
JPH0257175A (en) * | 1988-08-19 | 1990-02-26 | Morinaga & Co Ltd | Formed culture medium and production thereof |
NO901662L (en) * | 1989-04-17 | 1990-12-21 | Monsanto Co | DRY HERBICID PREPARATION WITH IMPROVED WATER SOLUBILITY. |
EP0441092A1 (en) | 1990-02-08 | 1991-08-14 | Niro Holding A/S | A method and apparatus for heat treating a particulate product |
US5254073A (en) * | 1990-04-27 | 1993-10-19 | Kapak Corporation | Method of making a vented pouch |
DK0474949T3 (en) | 1990-09-11 | 1995-01-30 | Niro Holding As | Method and apparatus for treating a powdered or particulate material or product with gas |
JPH0761259B2 (en) | 1990-10-08 | 1995-07-05 | 工業技術院長 | Extraction method of yeast intracellular substance |
JPH04198137A (en) | 1990-11-28 | 1992-07-17 | Sumitomo Pharmaceut Co Ltd | Production of dried sterile sodium carbonate |
JP2895641B2 (en) * | 1991-02-28 | 1999-05-24 | ハウス食品株式会社 | Animal cell growth promoter and animal cell growth method using the same |
US5700426A (en) | 1991-03-08 | 1997-12-23 | Foundation Nationale De Transfusion Sanguine | Method for decontaminating or sterilizing "in situ" a vacuum sealed container and device for implementing such method |
DK0507038T3 (en) | 1991-04-05 | 1994-10-17 | Niro Holding As | Apparatus with fluidizing bed and method for making same |
GB9107628D0 (en) | 1991-04-10 | 1991-05-29 | Moonbrook Limited | Preparation of diagnostic agents |
DE69231331T2 (en) | 1991-06-17 | 2001-02-22 | Life Technologies Inc | TECHNOLOGY FOR CONCENTRATING CULTURAL MEDIA |
US5155039A (en) | 1991-07-22 | 1992-10-13 | Chrisope Technologies, Inc. | Apparatus for methods for preserving, transporting storing, re-hydrating and delivering viable micro-organisms |
US5445792A (en) * | 1992-03-13 | 1995-08-29 | American Sterilizer Company | Optimum hydrogen peroxide vapor sterlization method |
US5325606A (en) | 1992-12-23 | 1994-07-05 | Niro A/S | Process and apparatus for drying and calcining sodium bicarbonate |
US5366696A (en) | 1993-01-07 | 1994-11-22 | 1077075 Ontario Inc. | Oxygenation apparatus for oxygenating a carrier liquid by spraying |
WO1994027645A1 (en) * | 1993-05-28 | 1994-12-08 | Porton International Plc | System for packaging and delivering of a sterile powder medium |
BR9406778A (en) | 1993-06-15 | 1996-03-19 | Commw Scient Ind Res Org | Fumigation process and apparatus |
DK75293D0 (en) * | 1993-06-24 | 1993-06-24 | Anhydro As | PROCEDURE AND PLANT FOR AN AGGLOMERATED PRODUCT |
US5631159A (en) | 1993-09-22 | 1997-05-20 | Creative Biomolecules, Inc. | Lipid-modified serum free media |
DK62994A (en) * | 1993-11-15 | 1995-05-16 | Niro Holding As | Apparatus and method for making an agglomerated material |
DE69312331T2 (en) | 1993-11-15 | 1998-02-19 | Niro Holding As | FLUID BED FOR PRODUCING AND / OR TREATING A PARTICULATE MATERIAL |
US5563068A (en) * | 1994-04-21 | 1996-10-08 | Genetic Therapy, Inc. | Bioreactor |
US5605889A (en) * | 1994-04-29 | 1997-02-25 | Pfizer Inc. | Method of administering azithromycin |
US5580856A (en) | 1994-07-15 | 1996-12-03 | Prestrelski; Steven J. | Formulation of a reconstituted protein, and method and kit for the production thereof |
US5662897A (en) * | 1994-07-27 | 1997-09-02 | U. Of Ga Research Foundation | Insect viruses, sequences, insecticidal compositions and methods of use |
US5733774A (en) * | 1995-02-02 | 1998-03-31 | Ecoscience Corporation | Method and composition for producing stable bacteria and bacterial formulations |
US5811406A (en) | 1995-06-07 | 1998-09-22 | Regents Of The University Of California | Dry powder formulations of polynucleotide complexes |
JPH11513248A (en) * | 1995-09-18 | 1999-11-16 | ミネソタ・マイニング・アンド・マニュファクチャリング・カンパニー | Thin-film culture plate device containing granular medium particles |
DE19535581C1 (en) * | 1995-09-25 | 1996-10-31 | Cpc Maizena Gmbh | Coagulated, flavoured egg cubes for adding e.g. to hot soup |
CA2235069C (en) | 1995-10-19 | 2010-12-14 | Advanced Reproduction Technologies, Inc. | Methods and compositions to improve germ cell and embryo survival and function |
SE507856C2 (en) | 1996-04-12 | 1998-07-20 | Acanova Ab | Heat remediation of seeds |
US6632648B1 (en) | 1996-05-14 | 2003-10-14 | Elan Drug Delivery Limited | Methods of terminal sterilization of fibrinogen |
US5773279A (en) | 1996-11-27 | 1998-06-30 | Neogen Corporation | Culture medium and method for repair of microbial cells |
US6383810B2 (en) | 1997-02-14 | 2002-05-07 | Invitrogen Corporation | Dry powder cells and cell culture reagents and methods of production thereof |
US20060003448A1 (en) * | 2003-12-30 | 2006-01-05 | Richard Fike | Dry powder cells and cell culture reagents and methods of production thereof |
US20040022666A1 (en) * | 1998-06-30 | 2004-02-05 | Invitrogen Corporation | Methods for reducing adventitious agents and toxins and cell culture reagents produced thereby |
US20060003447A1 (en) * | 2003-12-30 | 2006-01-05 | Richard Fike | Dry powder cells and cell culture reagents and methods of production thereof |
US6627426B2 (en) | 1997-02-14 | 2003-09-30 | Invitrogen Corporation | Methods for reducing adventitious agents and toxins and cell culture reagents produced thereby |
US6210715B1 (en) * | 1997-04-01 | 2001-04-03 | Cap Biotechnology, Inc. | Calcium phosphate microcarriers and microspheres |
US6045838A (en) * | 1998-08-10 | 2000-04-04 | Davis; Harold L. | Grape handling and storage bag |
US6206571B1 (en) | 1999-06-24 | 2001-03-27 | Alan D. Olin | Flexible bag with resealable pour spout |
NZ526173A (en) * | 2000-11-06 | 2005-03-24 | Invitrogen Corp | Dry powder cells and cell culture reagents and methods of production thereof |
EP2253699A1 (en) * | 2001-11-30 | 2010-11-24 | Life Technologies Corporation | Dry powder cell culture products containing lipid and methods of production thereof |
-
1998
- 1998-02-13 US US09/023,790 patent/US6383810B2/en not_active Expired - Lifetime
- 1998-02-17 WO PCT/US1998/002829 patent/WO1998036051A1/en not_active Application Discontinuation
- 1998-02-17 DK DK10175914.0T patent/DK2281873T3/en active
- 1998-02-17 DK DK09180261.1T patent/DK2186882T3/en active
- 1998-02-17 AT AT06002712T patent/ATE507283T1/en not_active IP Right Cessation
- 1998-02-17 ES ES10175914.0T patent/ES2516790T3/en not_active Expired - Lifetime
- 1998-02-17 DE DE69842243T patent/DE69842243D1/en not_active Expired - Lifetime
- 1998-02-17 AU AU66549/98A patent/AU6654998A/en not_active Abandoned
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- 1998-02-17 EP EP98908542A patent/EP1012234A4/en not_active Withdrawn
- 1998-02-17 DK DK06002712.5T patent/DK1702978T3/en active
- 1998-02-17 EP EP09180261.1A patent/EP2186882B1/en not_active Expired - Lifetime
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- 1998-02-17 EP EP06002712A patent/EP1702978B1/en not_active Expired - Lifetime
- 1998-02-17 EP EP10175914.0A patent/EP2281873B1/en not_active Expired - Lifetime
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2004
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2012
- 2012-04-20 US US13/452,498 patent/US20120276630A1/en not_active Abandoned
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