CA2255850A1 - Rotary thermocycling apparatus - Google Patents

Rotary thermocycling apparatus Download PDF

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Publication number
CA2255850A1
CA2255850A1 CA 2255850 CA2255850A CA2255850A1 CA 2255850 A1 CA2255850 A1 CA 2255850A1 CA 2255850 CA2255850 CA 2255850 CA 2255850 A CA2255850 A CA 2255850A CA 2255850 A1 CA2255850 A1 CA 2255850A1
Authority
CA
Canada
Prior art keywords
station
container
flat
stations
unit adapted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2255850
Other languages
French (fr)
Other versions
CA2255850C (en
Inventor
Roger George Laurence Wheatcroft
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agriculture and Agri Food Canada AAFC
Original Assignee
Agriculture and Agri Food Canada AAFC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CA002255850A priority Critical patent/CA2255850C/en
Application filed by Agriculture and Agri Food Canada AAFC filed Critical Agriculture and Agri Food Canada AAFC
Priority to AU10228/00A priority patent/AU1022800A/en
Priority to EP99953474A priority patent/EP1137490B1/en
Priority to PCT/CA1999/001035 priority patent/WO2000033962A1/en
Priority to DE69918160T priority patent/DE69918160T2/en
Priority to AT99953474T priority patent/ATE269163T1/en
Publication of CA2255850A1 publication Critical patent/CA2255850A1/en
Application granted granted Critical
Publication of CA2255850C publication Critical patent/CA2255850C/en
Priority to US09/876,428 priority patent/US6448066B1/en
Priority to US10/085,904 priority patent/US20020110899A1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Abstract

A rotary thermocycling apparatus, especially for biochemical reactions. The apparatus comprises a plurality of and especially at least four stations for receiving biochemical samples in a flat-bottomed container. Each station has a flat heated plate on which the container is placed and means to independently control the heated plate at a pre-determined temperature.
The apparatus has means to move each flat-bottomed container from one station to another station in a pre-determined sequence. At least two of the stations have a heating unit adapted to be lowered over a container located on the station, and at least one station has a spray unit adapted to spray liquid reagents) into a container located at that one station. A method is also described. The apparatus is used in, for example, polymerase chain reactions.
CA002255850A 1998-12-07 1998-12-07 Rotary thermocycling apparatus Expired - Fee Related CA2255850C (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
CA002255850A CA2255850C (en) 1998-12-07 1998-12-07 Rotary thermocycling apparatus
EP99953474A EP1137490B1 (en) 1998-12-07 1999-11-02 Rotary thermocycling apparatus
PCT/CA1999/001035 WO2000033962A1 (en) 1998-12-07 1999-11-02 Rotary thermocycling apparatus
DE69918160T DE69918160T2 (en) 1998-12-07 1999-11-02 TURNING thermocycler
AU10228/00A AU1022800A (en) 1998-12-07 1999-11-02 Rotary thermocycling apparatus
AT99953474T ATE269163T1 (en) 1998-12-07 1999-11-02 ROTARY THERMOCYCLER DEVICE
US09/876,428 US6448066B1 (en) 1998-12-07 2001-06-06 Rotary thermocycling apparatus
US10/085,904 US20020110899A1 (en) 1998-12-07 2002-02-27 Rotary thermocycling apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA002255850A CA2255850C (en) 1998-12-07 1998-12-07 Rotary thermocycling apparatus

Publications (2)

Publication Number Publication Date
CA2255850A1 true CA2255850A1 (en) 1999-12-21
CA2255850C CA2255850C (en) 2000-10-17

Family

ID=4163090

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002255850A Expired - Fee Related CA2255850C (en) 1998-12-07 1998-12-07 Rotary thermocycling apparatus

Country Status (7)

Country Link
US (2) US6448066B1 (en)
EP (1) EP1137490B1 (en)
AT (1) ATE269163T1 (en)
AU (1) AU1022800A (en)
CA (1) CA2255850C (en)
DE (1) DE69918160T2 (en)
WO (1) WO2000033962A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004029195A2 (en) * 2002-09-24 2004-04-08 U.S. Government As Represented By The Secretary Of The Army Portable thermocycler
US8993237B2 (en) 2002-01-30 2015-03-31 Applied Biosystems, Llc Device and method for thermal cycling

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US5959098A (en) 1996-04-17 1999-09-28 Affymetrix, Inc. Substrate preparation process
US6706875B1 (en) * 1996-04-17 2004-03-16 Affyemtrix, Inc. Substrate preparation process
DE19848515A1 (en) * 1998-10-21 2000-04-27 November Ag Molekulare Medizin Micro-titration plate recess has push-fit closure occupying space within reaction cavity, minimizing sample preparation and reaction time
US6719949B1 (en) * 2000-06-29 2004-04-13 Applera Corporation Apparatus and method for transporting sample well trays
FR2812306B1 (en) * 2000-07-28 2005-01-14 Gabriel Festoc POLYMERSIS CHAIN AMPLIFICATION SYSTEM OF TARGET NUCLEIC SEQUENCES
US6713023B2 (en) * 2001-06-29 2004-03-30 Agilent Technologies, Inc. Flow cell for chemical reactions
DE10325300A1 (en) * 2003-06-04 2005-01-20 Siemens Ag thermocycler
US7517498B2 (en) * 2003-08-19 2009-04-14 Agilent Technologies, Inc. Apparatus for substrate handling
DE102004022263A1 (en) * 2004-05-06 2005-12-15 Clondiag Chip Technologies Gmbh Apparatus and method for detecting molecular interactions
WO2005113741A1 (en) * 2004-05-12 2005-12-01 Board Of Regents Of University Of Nebraska Vortex tube thermocycler
US7622296B2 (en) * 2004-05-28 2009-11-24 Wafergen, Inc. Apparatus and method for multiplex analysis
US20060246493A1 (en) 2005-04-04 2006-11-02 Caliper Life Sciences, Inc. Method and apparatus for use in temperature controlled processing of microfluidic samples
WO2008091626A1 (en) 2007-01-22 2008-07-31 Wafergen, Inc. Apparatus for high throughput chemical reactions
US7726135B2 (en) 2007-06-06 2010-06-01 Greencentaire, Llc Energy transfer apparatus and methods
US20090200005A1 (en) * 2008-02-09 2009-08-13 Sullivan Shaun E Energy transfer tube apparatus, systems, and methods
WO2012033396A1 (en) * 2008-12-18 2012-03-15 Universiti Sains Malaysia A disposable multiplex polymerase chain reaction (pcr) chip and device
GB0909420D0 (en) * 2009-06-02 2009-07-15 Biochip Devises Pte Ltd Device for nucleic acid amplification
DE102009044431A1 (en) 2009-11-05 2011-06-22 FRIZ Biochem Gesellschaft für Bioanalytik mbH, 82061 Device for carrying out a PCR
JP5249988B2 (en) * 2010-05-07 2013-07-31 株式会社日立ハイテクノロジーズ Nucleic acid amplification apparatus and nucleic acid test apparatus using the same
CN103540518B (en) * 2010-07-23 2015-07-08 贝克曼考尔特公司 Test box
KR101302748B1 (en) * 2010-09-17 2013-08-30 한국식품연구원 System for multiplexing DNA amplification by non contact heating
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
DE102012016830B4 (en) * 2012-08-24 2021-08-26 Bruker Daltonics GmbH & Co. KG Preparation device for mass spectrometric samples
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
US10641772B2 (en) 2015-02-20 2020-05-05 Takara Bio Usa, Inc. Method for rapid accurate dispensing, visualization and analysis of single cells
EP3487616B1 (en) 2016-07-21 2023-08-09 Takara Bio USA, Inc. Multi-z imaging of wells of multi-well devices and liquid dispensing into the wells
CN108717028B (en) * 2018-05-30 2020-07-10 西南石油大学 Rotating disc type high-low temperature impact test device
US20210291190A1 (en) * 2018-07-13 2021-09-23 Deepdivebio, Inc. Thermocycler reaction control

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60241884A (en) * 1984-05-15 1985-11-30 Tokyo Daigaku Automation cycling reaction apparatus and automatic analyzer using same
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5038852A (en) 1986-02-25 1991-08-13 Cetus Corporation Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
CA2031912A1 (en) * 1989-12-22 1991-06-23 Robert Fred Pfost Heated cover device
WO1994008759A1 (en) * 1992-10-16 1994-04-28 Thomas Jefferson University Method and apparatus for robotically performing sanger dideoxynucleotide dna sequencing reactions
AU6104994A (en) * 1993-03-18 1994-10-11 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture Apparatus for the quantitative determination of particulate analytes
US5525300A (en) 1993-10-20 1996-06-11 Stratagene Thermal cycler including a temperature gradient block
JPH08196299A (en) * 1995-01-26 1996-08-06 Tosoh Corp Thermal cycling reaction apparatus and reaction vessel therefor
US5723294A (en) 1996-03-05 1998-03-03 Gull Laboratories Methods for detection and discrimination of multiple analytes using fluorescent technology
DE19646115C2 (en) * 1996-11-08 2000-05-25 Eppendorf Geraetebau Netheler Use of temperature control devices for temperature control of a temperature control block
ATE278471T1 (en) * 1998-05-04 2004-10-15 Hoffmann La Roche THERMOCYCLING APPARATUS WITH AN AUTOMATICALLY POSITIONABLE LID

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8993237B2 (en) 2002-01-30 2015-03-31 Applied Biosystems, Llc Device and method for thermal cycling
US20150165439A1 (en) * 2002-01-30 2015-06-18 Applied Biosystems, Llc Device and method for Thermal Cycling
US9889448B2 (en) * 2002-01-30 2018-02-13 Applied Biosystems, Llc Device and method for thermal cycling
WO2004029195A2 (en) * 2002-09-24 2004-04-08 U.S. Government As Represented By The Secretary Of The Army Portable thermocycler
WO2004029195A3 (en) * 2002-09-24 2004-07-15 Us Gov Sec Army Portable thermocycler
US6875602B2 (en) 2002-09-24 2005-04-05 The United States Of America As Represented By The Secretary Of The Army Portable thermocycler

Also Published As

Publication number Publication date
EP1137490B1 (en) 2004-06-16
CA2255850C (en) 2000-10-17
DE69918160D1 (en) 2004-07-22
WO2000033962A1 (en) 2000-06-15
US20020127709A1 (en) 2002-09-12
EP1137490A1 (en) 2001-10-04
ATE269163T1 (en) 2004-07-15
US20020110899A1 (en) 2002-08-15
AU1022800A (en) 2000-06-26
DE69918160T2 (en) 2005-07-07
US6448066B1 (en) 2002-09-10

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