CA2207608A1 - Isolation of nucleic acid - Google Patents

Isolation of nucleic acid

Info

Publication number
CA2207608A1
CA2207608A1 CA002207608A CA2207608A CA2207608A1 CA 2207608 A1 CA2207608 A1 CA 2207608A1 CA 002207608 A CA002207608 A CA 002207608A CA 2207608 A CA2207608 A CA 2207608A CA 2207608 A1 CA2207608 A1 CA 2207608A1
Authority
CA
Canada
Prior art keywords
nucleic acid
sample
isolation
support
bound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002207608A
Other languages
French (fr)
Other versions
CA2207608C (en
Inventor
Arne Helge Deggerdal
Frank Larsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies AS
Original Assignee
Dynal As
Arne Helge Deggerdal
Frank Larsen
Dynal Asa
Dynal Biotech Asa
Dynal Biotech As
Invitrogen Dynal As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=10765877&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2207608(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dynal As, Arne Helge Deggerdal, Frank Larsen, Dynal Asa, Dynal Biotech Asa, Dynal Biotech As, Invitrogen Dynal As filed Critical Dynal As
Publication of CA2207608A1 publication Critical patent/CA2207608A1/en
Application granted granted Critical
Publication of CA2207608C publication Critical patent/CA2207608C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

Abstract

The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a detergent and a solid support, whereby soluble nucleic acid in said sample is bound to the support, and separating said support with bound nucleic acid from the sample. Where the method of the invention is used to isolate DNA, it may conveniently be coupled with a further step to isolate RNA from the same sample.
CA002207608A 1994-12-12 1995-12-12 Isolation of nucleic acid Expired - Fee Related CA2207608C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9425138.6 1994-12-12
GBGB9425138.6A GB9425138D0 (en) 1994-12-12 1994-12-12 Isolation of nucleic acid
PCT/GB1995/002893 WO1996018731A2 (en) 1994-12-12 1995-12-12 Isolation of nucleic acid

Publications (2)

Publication Number Publication Date
CA2207608A1 true CA2207608A1 (en) 1996-06-20
CA2207608C CA2207608C (en) 2009-04-07

Family

ID=10765877

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002207608A Expired - Fee Related CA2207608C (en) 1994-12-12 1995-12-12 Isolation of nucleic acid

Country Status (9)

Country Link
US (8) US20040215011A1 (en)
EP (1) EP0796327B1 (en)
JP (1) JP3787354B2 (en)
AT (1) ATE272110T1 (en)
AU (1) AU706211B2 (en)
CA (1) CA2207608C (en)
DE (1) DE69533317T2 (en)
GB (1) GB9425138D0 (en)
WO (1) WO1996018731A2 (en)

Families Citing this family (166)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
JP2965131B2 (en) 1995-07-07 1999-10-18 東洋紡績株式会社 Magnetic carrier for nucleic acid binding and nucleic acid isolation method using the same
US5990302A (en) * 1996-07-12 1999-11-23 Toyo Boseki Kabushiki Kaisha Method for isolating ribonucleic acid
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
GB9709728D0 (en) * 1997-05-13 1997-07-02 Dynal As Single step method
EP1027145A4 (en) 1997-09-17 2004-08-25 Gentra Systems Inc Apparatuses and methods for isolating nucleic acid
EP1053357B1 (en) * 1998-02-02 2008-05-21 QIAGEN North American Holdings, Inc. Processes for isolating, amplifying and characterizing dna
EP1053311B1 (en) * 1998-02-02 2007-11-28 QIAGEN North American Holdings, Inc. Compositions and methods for using a lysing matrix for isolating dna
EP1950312A1 (en) 1998-02-02 2008-07-30 QIAGEN North American Holdings, Inc. Processes for isolating, amplifying and characterizing DNA
US6534262B1 (en) 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
AU764494B2 (en) * 1998-09-10 2003-08-21 Whatman Plc Product and method for separation of a sample containing multiple sources of genetic material using a solid medium
US5973138A (en) * 1998-10-30 1999-10-26 Becton Dickinson And Company Method for purification and manipulation of nucleic acids using paramagnetic particles
FI990082A0 (en) * 1999-01-18 1999-01-18 Labsystems Oy Purification process using magnetic particles
US7790865B1 (en) 1999-02-02 2010-09-07 Qiagen North American Holdings, Inc Eluting reagents, methods and kits for isolating DNA
ES2308865T3 (en) * 1999-03-11 2008-12-01 Whatman, Inc. SOLID ENVIRONMENT AND PROCEDURE FOR STORAGE AND FAST PURIFICATION OF NUCLEIC ACID.
AU4367500A (en) * 1999-04-21 2000-11-02 Annovis, Inc. Magnetic dna extraction kit for plants
ATE320443T1 (en) * 1999-07-15 2006-04-15 Qiagen Gmbh METHOD FOR SEPARATING PARTICLE SUBSTRATES FROM A SOLUTION BY MINIMIZING PARTICLE LOSS
US6936414B2 (en) * 1999-12-22 2005-08-30 Abbott Laboratories Nucleic acid isolation method and kit
GB0001450D0 (en) 2000-01-21 2000-03-08 Genpoint As Cell isolation method
GB0013658D0 (en) * 2000-06-05 2000-07-26 Dynal Asa Nucleic acid isolation
US20050239125A1 (en) * 2000-09-06 2005-10-27 Hodge Timothy A Methods for genotype screening
EP1978110B1 (en) * 2000-09-06 2010-05-26 Transnetyx, Inc. Computer-based method and system for screening genomic DNA
KR20020065509A (en) * 2000-09-06 2002-08-13 티모시 에이. 호지 System, method and apparatus for transgenic and targeted mutagenesis screening
EP1349951A4 (en) * 2001-01-09 2005-08-24 Whitehead Biomedical Inst Methods and reagents for the isolation of nucleic acids
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
JPWO2003006650A1 (en) * 2001-07-09 2004-11-04 旭化成株式会社 Nucleic acid purification method and detection method using nonwoven fabric
US7893228B2 (en) 2001-10-12 2011-02-22 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
US20050032105A1 (en) 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
US7148343B2 (en) 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
KR100445560B1 (en) * 2001-10-31 2004-08-21 (주)바이오넥스 Method of manufacturing kit for isolating nucleic acids or biological materials, kit manufactured by the method, and apparatus using the kit
US7208271B2 (en) * 2001-11-28 2007-04-24 Applera Corporation Compositions and methods of selective nucleic acid isolation
JP4148900B2 (en) 2002-01-08 2008-09-10 エフ．ホフマン−ラ ロシュ アーゲー Use of silica materials in amplification reactions
JP2005538929A (en) 2002-01-16 2005-12-22 ダイナル バイオテック エイエスエイ Methods for isolating nucleic acids and proteins from a single sample
US7786295B2 (en) 2002-02-15 2010-08-31 Qiegen North American Holdings, Inc Method to isolate DNA
GB0212826D0 (en) 2002-05-31 2002-07-10 Dna Res Innovations Ltd Materials and methods relating to polyions and substance delivery
GB0215185D0 (en) 2002-07-01 2002-08-07 Genovision As Binding a target substance
US20040029166A1 (en) * 2002-07-29 2004-02-12 Jsr Corporation Nucleic acid-separating method and nucleic acid-extracting reagent
ES2320875T5 (en) † 2002-10-04 2012-10-30 Ge Healthcare Bio-Sciences Corp. Procedures and materials for using chemical compounds as a tool for the storage of nucleic acids on means of nucleic acid purification systems
US20040180445A1 (en) * 2003-03-12 2004-09-16 Domanico Michael J. Methods and compositions for purification of nucleic acid from a host cell
WO2004108925A1 (en) * 2003-06-04 2004-12-16 Qiagen As Method for sequentially isolating dna and rna from the same nucleic acid-containing sample
CN1878866B (en) † 2003-07-25 2012-08-01 安比恩股份有限公司 Methods and compositions for preparing RNA from a fixed sample
EP2402089A1 (en) 2003-07-31 2012-01-04 Handylab, Inc. Processing particle-containing samples
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
WO2005108620A2 (en) * 2004-05-03 2005-11-17 Handylab, Inc. Processing polynucleotide-containing samples
EP1756136B1 (en) 2004-05-21 2014-10-29 Mo Bio Laboratories, Inc. Kits and processes for removing contaminants from nucleic acids in environmental and biological samples
WO2006004611A2 (en) * 2004-06-25 2006-01-12 Invitrogen Corporation Separation of nucleic acid
EP1776458A2 (en) 2004-07-30 2007-04-25 Agencourt Bioscience Corporation Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers
KR20130143677A (en) 2004-11-05 2013-12-31 퀴아젠 노쓰 아메리칸 홀딩즈, 인크. Compositions and methods for purifying nucleic acids from stabilization reagents
US20060166223A1 (en) * 2005-01-26 2006-07-27 Reed Michael W DNA purification and analysis on nanoengineered surfaces
WO2006088907A2 (en) 2005-02-15 2006-08-24 University Of Virginia Patent Foundation Nucleic acid isolation methods and materials and devices thereof
CA2842232C (en) * 2005-03-10 2015-01-27 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
US20100063263A1 (en) * 2005-06-23 2010-03-11 Siemens Medical Solutions Diagnostics Gmbh Magnetic particles with a closed ultrathin silica layer, method for the production thereof and their use
DE102005047736B4 (en) * 2005-09-29 2008-08-14 Aj Innuscreen Gmbh Method and system for isolating nucleic acids from any complex starting materials
JP5415253B2 (en) 2006-03-24 2014-02-12 ハンディラブ・インコーポレーテッド Integrated system for processing microfluidic samples and methods of use thereof
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US20100216657A1 (en) * 2006-05-16 2010-08-26 Arcxis Biotechnologies, Inc. Pcr-free sample preparation and detection systems for high speed biologic analysis and identification
US8163535B2 (en) * 2006-06-26 2012-04-24 Blood Cell Storage, Inc. Devices and processes for nucleic acid extraction
US7608399B2 (en) * 2006-06-26 2009-10-27 Blood Cell Storage, Inc. Device and method for extraction and analysis of nucleic acids from biological samples
US20080003574A1 (en) * 2006-06-28 2008-01-03 Sigma-Aldrich Co. Kits for RNA extraction
US10131935B2 (en) 2006-07-11 2018-11-20 Aj Innuscreen Gmbh Method for parallel isolation of viral nucleic acids
DE102006032610C5 (en) * 2006-07-11 2016-09-15 Aj Innuscreen Gmbh Method for the parallel isolation of viral nucleic acids
WO2008055915A2 (en) 2006-11-06 2008-05-15 Clondiag Gmbh Device and process for assays using binding members
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
WO2008061165A2 (en) * 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic cartridge and method of making same
WO2008116182A1 (en) * 2007-03-21 2008-09-25 Ibis Biosciences, Inc. Reagents for nucleic acid purification
CA2629589C (en) 2007-04-20 2016-03-29 F.Hoffmann-La Roche Ag Isolation and purification of nucleic acid molecules with a solid phase
DK2171098T3 (en) 2007-06-29 2018-05-22 Becton Dickinson Co PROCEDURES FOR EXTRACTION AND CLEANING COMPONENTS IN BIOLOGICAL SAMPLES
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
CA2693654C (en) 2007-07-13 2018-02-13 Handylab, Inc. Polynucleotide capture materials, and methods of using same
EP2610007B1 (en) 2007-07-23 2018-12-05 CLONDIAG GmbH Assays
EP2191012A1 (en) * 2007-09-21 2010-06-02 Streck, Inc. Nucleic acid isolation in preserved whole blood
GB0719022D0 (en) * 2007-09-28 2007-11-07 Mole Genetics As Isolation method
US8478544B2 (en) 2007-11-21 2013-07-02 Cosmosid Inc. Direct identification and measurement of relative populations of microorganisms with direct DNA sequencing and probabilistic methods
ES2694573T3 (en) 2007-11-21 2018-12-21 Cosmosid Inc. Genome identification system
CN104531673B (en) 2008-02-15 2018-03-16 生命科技公司 Method and kit for DNA extractions
DE102008010692A1 (en) 2008-02-22 2009-08-27 Qiagen Gmbh New matrices, processes for their preparation and their use in methods of isolating biomolecules
DE102008010693A1 (en) 2008-02-22 2009-08-27 Qiagen Gmbh New matrix materials, processes for their preparation and their use in methods of isolating biomolecules
CL2009000647A1 (en) * 2008-04-04 2010-06-04 Chugai Pharmaceutical Co Ltd Pharmaceutical composition for treating or preventing liver cancer comprising a combination of a chemotherapeutic agent and an anti-glypican 3 antibody; agent for attenuating a side effect comprising said antibody; method of treating or preventing liver cancer of a subject.
DE102008029356A1 (en) * 2008-06-20 2009-12-24 Siemens Healthcare Diagnostics Gmbh Process for the purification of nucleic acids, in particular from fixed tissue
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
CA2742598A1 (en) * 2008-11-04 2010-05-14 Blood Cell Storage, Inc. Nucleic acid extraction on curved glass surfaces
US20110275126A1 (en) * 2009-01-16 2011-11-10 Arkray, Inc. Method for Producing Nucleic Acid Sample and Method for Producing Nucleic Acid Amplification Product Using the Same
US8790916B2 (en) 2009-05-14 2014-07-29 Genestream, Inc. Microfluidic method and system for isolating particles from biological fluid
US8697435B2 (en) * 2009-08-31 2014-04-15 Mbio Diagnostics, Inc. Integrated sample preparation and analyte detection
US20110223588A1 (en) * 2010-03-09 2011-09-15 Biosample Llc Solid Phase Nucleic Acid Extraction From Small Sample Volumes, and Release of Controlled Quantities
DK2588609T3 (en) 2010-06-29 2018-03-19 Exscale Biospecimen Solutions Ab METHOD AND KIT FOR SEQUENTIAL ISOLATION OF NUCLEOTIDE SPECIES FROM A SAMPLE
US8747084B2 (en) 2010-07-21 2014-06-10 Aperia Technologies, Inc. Peristaltic pump
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
GB201020095D0 (en) 2010-11-26 2011-01-12 Invitrogen Dynal As Nucleic acid preparation method
JP2014503222A (en) * 2011-01-18 2014-02-13 エバリスト ジェノミックス， インコーポレイテッド Prognostic signs of colorectal cancer recurrence
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
CA3082652A1 (en) 2011-04-15 2012-10-18 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
WO2012159063A2 (en) 2011-05-19 2012-11-22 Blood Cell Strorage, Inc. Gravity flow fluidic device for nucleic acid extraction
EP2546199A1 (en) 2011-07-14 2013-01-16 Université Catholique De Louvain Solid iron oxide support for isolation of microorganisms
JP5924888B2 (en) * 2011-08-26 2016-05-25 関東化學株式会社 Nucleic acid extraction method, nucleic acid extraction reagent kit, and nucleic acid extraction reagent
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
DK2761305T3 (en) 2011-09-30 2017-11-20 Becton Dickinson Co United Reagent Strip
EP2773892B1 (en) 2011-11-04 2020-10-07 Handylab, Inc. Polynucleotide sample preparation device
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
WO2013067509A1 (en) * 2011-11-04 2013-05-10 Bio-Rad Laboratories, Inc. Simultaneous purification of cell components
WO2013091102A1 (en) * 2011-12-21 2013-06-27 Geneohm Sciences Canada Inc. Enrichment & isolation of microbial cells & microbial nucleic acids from a biological sample
RU2658773C2 (en) 2012-02-03 2018-06-22 Бектон, Дикинсон Энд Компани System and method of implementation of automated assays on plurality of biological samples
JP6333737B2 (en) 2012-02-09 2018-05-30 ライフ テクノロジーズ コーポレーション Conjugated polymer particles and method for producing the same
US9062079B2 (en) 2012-02-09 2015-06-23 Life Technologies Corporation Hydrophobic diacrylamide compound
KR102019297B1 (en) 2012-02-09 2019-09-06 라이프 테크놀로지스 코포레이션 Hydrophilic polymeric particles and methods for making same
CN104254390B (en) 2012-02-15 2017-05-10 生物辐射实验室股份有限公司 electronic control of pH and ionic strength
WO2013126620A1 (en) 2012-02-23 2013-08-29 Dow Agrosciences Llc Automation of barrier-based plant nucleic acid and protein extraction
US9222473B2 (en) 2012-03-20 2015-12-29 Aperia Technologies, Inc. Passive pressure regulation mechanism
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
WO2014018195A1 (en) 2012-06-21 2014-01-30 Monsanto Technology Llc Lysis buffer and methods for extraction of dna from plant material
CN104583421A (en) 2012-07-19 2015-04-29 阿瑞奥萨诊断公司 Multiplexed sequential ligation-based detection of genetic variants
CN103571824B (en) 2012-08-09 2016-04-13 财团法人工业技术研究院 Compositions and methods for isolating nucleic acids
EP2891716B1 (en) 2012-08-28 2018-03-28 Biocube System Inc. Porous solid phase for rapidly isolating biological molecules for nucleic acid amplification reaction from biological sample, and use thereof
GB201216387D0 (en) 2012-09-13 2012-10-31 Ge Healthcare Uk Ltd Solid matrix for one step nucleic acid amplification
US10245908B2 (en) 2016-09-06 2019-04-02 Aperia Technologies, Inc. System for tire inflation
US11453258B2 (en) 2013-03-12 2022-09-27 Aperia Technologies, Inc. System for tire inflation
US9604157B2 (en) 2013-03-12 2017-03-28 Aperia Technologies, Inc. Pump with water management
US10144254B2 (en) 2013-03-12 2018-12-04 Aperia Technologies, Inc. Tire inflation system
US10052428B2 (en) 2013-03-15 2018-08-21 Fenwal, Inc. Methods and systems for the filterless reduction of leukocytes in a biological fluid
AU2014332956B2 (en) * 2013-10-07 2018-01-25 Mitsui Chemicals, Inc. PCR primer set for bacterial DNA amplification, kit for detecting and/or identifying bacterial species, and method for detecting and/or identifying bacterial species
EP3653723B1 (en) 2014-02-10 2022-07-20 Zymo Research Corporation Methods for nucleic acid capture
US20150252407A1 (en) * 2014-03-09 2015-09-10 Nvigen, Inc. Nanostructure and methods of nucleic acid isolation
SG11201609008PA (en) 2014-04-29 2016-11-29 Accudx Corp A novel affinity matrix and devices for isolation and purification of rna and dna for point of care molecular devices
US9879223B2 (en) 2014-07-30 2018-01-30 Fenwal, Inc. Methods and systems for the reduction of leukocytes in a biological fluid
JP6437250B2 (en) 2014-09-03 2018-12-12 株式会社ミズホメディー Pretreatment method and nucleic acid extraction kit used therefor
WO2016057891A1 (en) 2014-10-09 2016-04-14 Promega Corporation Method of isolating nucleic acid
WO2016077294A1 (en) 2014-11-14 2016-05-19 Corning Incorporated Methods and kits for post-ivt rna purification
WO2016099999A1 (en) * 2014-12-18 2016-06-23 Ge Healthcare Uk Limited Analyte detection on a solid support by nucleic acid amplification coupled to an immunoassay
GB201504459D0 (en) * 2015-03-17 2015-04-29 Moorlodge Biotech Ventures Ltd Isolation of DNA
US9422596B1 (en) 2015-04-27 2016-08-23 Norgen Biotek Corp. Methods and columns for nucleic acid purification
EP3317426B1 (en) 2015-07-02 2020-01-15 Life Technologies Corporation Conjugation of carboxyl functional hydrophilic beads
EP3702383B1 (en) 2015-07-02 2023-12-27 Life Technologies Corporation Polymer substrates formed from carboxy functional acrylamide
US10150992B2 (en) 2015-07-06 2018-12-11 Life Technologies Corporation Substrates and methods useful in sequencing
US11242518B2 (en) 2015-09-04 2022-02-08 QIAGEN Sciences, LLP Methods for co-isolation of nucleic acids and proteins
CN108026570A (en) * 2015-09-11 2018-05-11 康宁股份有限公司 The composition and method of purification of nucleic acid from blood sample
US20180135040A1 (en) 2016-02-16 2018-05-17 Life Magnetics, Inc. Methods for separating nucleic acids with graphene coated magnetic beads
WO2017184984A1 (en) * 2016-04-21 2017-10-26 Cell Data Sciences, Inc. Biomolecule processing from fixed biological samples
US11091792B2 (en) * 2016-05-04 2021-08-17 Children's Hospital & Research Center At Oakland Rapid extraction of nucleic acids from clinical samples for downstream applications
DE102016121483B4 (en) 2016-11-09 2020-06-18 Axagarius Gmbh & Co. Kg Particulate solid composite material for nucleic acid purification, containing magnetic nanoparticles, process for its production and its use
US11129673B2 (en) 2017-05-05 2021-09-28 Uptake Medical Technology Inc. Extra-airway vapor ablation for treating airway constriction in patients with asthma and COPD
JP7323532B2 (en) 2017-09-18 2023-08-08 フェーズ サイエンティフィック インターナショナル リミテッド Methods of Using Aqueous Two-Phase Systems for Isolation, Purification and/or Concentration of Short Nucleic Acid Fragments
WO2020112686A1 (en) 2018-11-27 2020-06-04 Aperia Technologies, Inc. Hub-integrated inflation system
TWI733302B (en) * 2020-01-09 2021-07-11 創想生物科技有限公司 A method of isolating nucleic acids and the system thereof
KR20220075978A (en) 2020-11-30 2022-06-08 (주)바이오니아 Method of Separating Nucleic Acid by Using Binding Buffer with Chemicals Having Low-Mid Dielectric Constant
CN112626174A (en) * 2020-12-16 2021-04-09 广州源井生物科技有限公司 Cell lysis solution and application thereof

Family Cites Families (229)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US873102A (en) 1907-06-21 1907-12-10 William W Sly Clutch.
SE328534B (en) * 1965-08-27 1970-09-21 Astra Ab
US4055469A (en) 1976-12-10 1977-10-25 Eastman Kodak Company Purification of microbial enzyme extracts using synthetic polyelectrolytes
JPS598164B2 (en) 1977-11-30 1984-02-23 三菱重工業株式会社 Tanning equipment for filter materials such as dust masks
AU530410B2 (en) * 1978-02-21 1983-07-14 Sintef Preparing aqueous emulsions
JPS5940227B2 (en) * 1980-06-24 1984-09-28 富士通株式会社 Reactive sputtering method
DE3132491A1 (en) * 1980-08-22 1982-07-01 Laboratorium Prof. Dr. Rudolf Berthold, 7547 Wildbad METHOD FOR PERFORMING ANALYTICAL DETERMINATIONS BY MEANS OF THE CHEMILUMINESCENCE METHOD AND APPLICATION OF THE METHOD FOR IMMUNASSAYS
US4379843A (en) * 1981-01-26 1983-04-12 Peter Cashion Immobilization of polynucleotides and polypeptides with tritylated polysaccharides
US5055556A (en) 1981-10-06 1991-10-08 The Board Of Trustees Of The Leland Stanford Jr. Univ. Fluorescent conjugates for analysis of molecules and cells
IL65131A0 (en) * 1982-02-28 1982-04-30 Yeda Res & Dev Process for the production of agarose-polyaldehyde beads and their biological applications
NO155316C (en) * 1982-04-23 1987-03-11 Sintef PROCEDURE FOR MAKING MAGNETIC POLYMER PARTICLES.
US4695537A (en) * 1982-05-21 1987-09-22 The University Of Tennessee Research Corp. Particles sensitized with detergent-treated antigen for agglutination immunoassay
GR79124B (en) 1982-12-22 1984-10-02 Genentech Inc
US4861705A (en) 1983-01-31 1989-08-29 Yeda Research And Development Company, Ltd. Method for removing components of biological fluids
DE3310337A1 (en) 1983-03-22 1984-09-27 Europäisches Laboratorium für Molekularbiologie (EMBL), 6900 Heidelberg METHOD FOR CARRYING OUT HYBRIDIZATION REACTIONS
US4672040A (en) * 1983-05-12 1987-06-09 Advanced Magnetics, Inc. Magnetic particles for use in separations
US4648975A (en) * 1983-08-17 1987-03-10 Pedro B. Macedo Process of using improved silica-based chromatographic supports containing additives
US4920061A (en) * 1984-03-02 1990-04-24 The University Of Texas System Biological magnetic colloids
US4652517A (en) 1984-06-11 1987-03-24 Diagnostic Research Limited Partnership Methods for the in vitro detection and identification of unknown pathogens or genetic entities
CA1327568C (en) 1984-07-06 1994-03-08 Jurg Merz Ionically modified polysaccharides, process for their preparation and the use thereof
GB8501671D0 (en) * 1985-01-23 1985-02-27 Allen G J Immunoassay
GB8507706D0 (en) 1985-03-25 1985-05-01 Genetics Int Inc Magnetic nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4897444A (en) * 1985-05-31 1990-01-30 The Research Foundation Of The State University Of New York Immobilized fluorogenic substrates for enzymes; and processes for their preparation
US4710472A (en) 1985-09-25 1987-12-01 The United States Of America As Represented By The Secretary Of The Navy Magnetic separation device
US4889916A (en) 1985-11-19 1989-12-26 The Johns Hopkins University Protein label and drug delivery system
US5076950A (en) * 1985-12-20 1991-12-31 Syntex (U.S.A.) Inc. Magnetic composition for particle separation
US4772550A (en) 1986-02-10 1988-09-20 Miles Inc. Heterogeneous specific binding assay employing an aggregatable binding reagent
US4871433A (en) 1986-04-04 1989-10-03 Materials Research Corporation Method and apparatus for improving the uniformity ion bombardment in a magnetron sputtering system
US6881536B1 (en) * 1986-04-30 2005-04-19 Bioveris Corporation Particle based electrochemiluminescent assays
FR2600655B1 (en) 1986-06-30 1988-10-21 Centre Nat Rech Scient PROTEIN FRACTION EXTRACTED FROM GROWTH FACTOR ACTIVITIES, OBTAINING AND APPLICATION AS A STIMULATION AND POTENTIALIZING AGENT FOR GROWTH FACTOR ACTIVITIES
US4843012A (en) * 1986-09-17 1989-06-27 Genetics Institute, Inc. Novel composition for nucleic acid purification
DE3639949A1 (en) 1986-11-22 1988-06-09 Diagen Inst Molekularbio METHOD FOR SEPARATING LONG CHAIN NUCLEIC ACIDS
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US4767670A (en) 1987-01-21 1988-08-30 E. I. Du Pont De Nemours And Company Chromatographic supports for separation of oligonucleotides
US4874813A (en) 1987-02-09 1989-10-17 Shannessy Daniel J O Proteins bound to a marker or solid phase support matrix using a hydrazone linkage
NO162946C (en) * 1987-08-21 1990-03-14 Otto Soerensen DEVICE FOR MAGNETIC SEPARATION OF CELLS.
DE3850273T2 (en) 1987-03-02 1994-09-29 Gen Probe Inc Polycationic carriers for the purification, separation and hybridization of nucleic acid.
US5599667A (en) * 1987-03-02 1997-02-04 Gen-Probe Incorporated Polycationic supports and nucleic acid purification separation and hybridization
US5079155A (en) * 1987-03-02 1992-01-07 E. I. Du Pont De Nemours And Company Fluorocarbon polymer support for chromatographic separations, diagnostic assays and enzyme immobilization
DE3717211A1 (en) 1987-05-22 1988-12-01 Diagen Inst Molekularbio DEVICE AND METHOD FOR SEPARATING AND CLEANING MOLECULES
US4921805A (en) * 1987-07-29 1990-05-01 Life Technologies, Inc. Nucleic acid capture method
CA1297432C (en) 1987-07-29 1992-03-17 Gulilat Gebeyehu Nucleic acid capture reagent
FR2619568B1 (en) 1987-08-21 1989-07-07 Centre Nat Rech Scient POLYSTYRENE-DERIVED POLYMERS, PROCESSES FOR THE PREPARATION THEREOF AND APPLICATIONS THEREOF FOR THE ANALYSIS AND PURIFICATION OF ORGANIC MOLECULES
US4908318A (en) * 1987-09-04 1990-03-13 Integrated Genetics, Inc. Nucleic acid extraction methods
US5585481A (en) 1987-09-21 1996-12-17 Gen-Probe Incorporated Linking reagents for nucleotide probes
US4923978A (en) * 1987-12-28 1990-05-08 E. I. Du Pont De Nemours & Company Process for purifying nucleic acids
GB8800702D0 (en) * 1988-01-13 1988-02-10 Nycomed As Test method & reagent kit therefor
US5183809A (en) * 1990-02-15 1993-02-02 The Trustees Of The University Of Pennsylvania/Childrens Hospital Corporation Cyclodextrin polymers and cyclodextrins immobilized on a solid surface
US4875045A (en) 1988-03-09 1989-10-17 Northern Telecom Limited Variable gain encoder apparatus and method
EP0338591A3 (en) 1988-04-21 1991-09-04 Microprobe Corporation Nucleic acid extraction method
US4952519A (en) 1988-05-02 1990-08-28 E. I. Du Pont De Nemours And Company Protein immobilization with poly(ethyleneimine) derivatized with a hydroprobic group
DE3921498A1 (en) * 1988-09-28 1990-03-29 Bayer Ag POLYMER-TIED DYES, METHOD FOR THE PRODUCTION AND USE THEREOF
US5807527A (en) 1991-05-29 1998-09-15 Flinders Technologies Pty. Ltd. Solid medium and method for DNA storage
CA1335181C (en) 1988-10-11 1995-04-11 R. Alan Hardwick System for selective cell separation from cell concentrate
IE883226L (en) 1988-10-25 1990-04-25 Dna Prep Galway Ltd S 22 Separation of nucleic acids from protein material using¹cation exchangers
US5028696A (en) 1988-10-28 1991-07-02 Torres Anthony R Ion exchange and separation method
US6448091B1 (en) 1988-11-03 2002-09-10 Igen International, Inc. Method and apparatus for improved luminescence assays using particle concentration chemiluminescence detection
US5512439A (en) * 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
GB8827160D0 (en) 1988-11-21 1988-12-29 Apothekernes Lab Detection & quantitative determination of rna & dna
CA2005927A1 (en) * 1988-12-21 1990-06-21 Chander Bahl Method of preparing nucleotide probes using a bridging complement
AU5040590A (en) 1989-01-23 1990-08-13 Invitron Corporation Method for removing dna from protein preparations
JP2910929B2 (en) * 1989-03-23 1999-06-23 浜松ホトニクス株式会社 Surface modification method for particles composed of magnetic particles
NL8900725A (en) 1989-03-23 1990-10-16 Az Univ Amsterdam METHOD AND COMBINATION OF AGENTS FOR INSULATING NUCLEIC ACID.
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US4957605A (en) 1989-04-17 1990-09-18 Materials Research Corporation Method and apparatus for sputter coating stepped wafers
US5334499A (en) 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
US5126028A (en) 1989-04-17 1992-06-30 Materials Research Corporation Sputter coating process control method and apparatus
US6010867A (en) * 1989-04-19 2000-01-04 Ibiden Co., Ltd. Reagent for biomaterials assay, preparation method thereof, and assay method
DE3916595A1 (en) 1989-05-22 1990-11-29 Boehringer Mannheim Gmbh METHOD FOR NON-RADIOACTIVE MEASURING OF NUCLEIC ACID SYNTHESIS IN EUKARYONTIC CELLS
NO168811C (en) 1989-05-31 1992-04-08 Dynal As Separation device for separating magnetizable particles
US5124444A (en) * 1989-07-24 1992-06-23 Microprobe Corporation Lactam-containing compositions and methods useful for the extraction of nucleic acids
DE69007716T2 (en) 1989-08-07 1994-07-14 Baker J T Inc Solid supports made of quaternized PEI silica for chromatography.
US5032281A (en) * 1989-08-09 1991-07-16 Daicel Chemical Industries, Ltd. Separating membrane and separation method
US5128247A (en) * 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
US5084169A (en) * 1989-09-19 1992-01-28 The University Of Colorado Foundation, Inc. Stationary magnetically stabilized fluidized bed for protein separation and purification
US5433847A (en) * 1989-11-01 1995-07-18 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Radial flow chromatography
US4997932A (en) * 1989-11-13 1991-03-05 Boehringer Mannheim Corporation Method and kit for purifying nucleic acids
AU658132B2 (en) 1989-11-13 1995-04-06 Children's Medical Center Corporation Non-invasive method for isolation and detection of fetal DNA
US5641628A (en) * 1989-11-13 1997-06-24 Children's Medical Center Corporation Non-invasive method for isolation and detection of fetal DNA
US5000635A (en) * 1989-11-17 1991-03-19 The Boeing Company Spring-loaded outboard side lock
DE69029740T2 (en) 1989-11-30 1997-07-10 Pharmacia Biotech Ab NEW METHOD FOR DETECTING A SPECIFIC NUCLEIC ACID SEQUENCE IN A CELL SAMPLE
US5523231A (en) * 1990-02-13 1996-06-04 Amersham International Plc Method to isolate macromolecules using magnetically attractable beads which do not specifically bind the macromolecules
GB9003253D0 (en) 1990-02-13 1990-04-11 Amersham Int Plc Precipitating polymers
IT1240870B (en) * 1990-02-14 1993-12-17 Talent PROCEDURE FOR THE EXTRACTION AND PURIFICATION OF HUMAN GENOMIC DNA
CA2082936C (en) 1990-05-15 2003-09-23 Peter O. G. Arrhenius Fluorescent porphyrin, and fluorescent phthalocyanine-polyethylene glycol, polyol, and saccharide derivatives as fluorescent probes
US5234924A (en) 1990-09-26 1993-08-10 Adir Et Compagnie Benzothiazine and benzothiazole compounds useful as analgesics
DE4034036C2 (en) 1990-10-26 1994-03-03 Diagen Inst Molekularbio Device and method for isolating nucleic acids from cell suspensions
AU8951191A (en) * 1990-10-29 1992-05-26 Dekalb Plant Genetics Isolation of biological materials using magnetic particles
US5234824A (en) 1990-11-13 1993-08-10 Specialty Laboratories, Inc. Rapid purification of DNA
US5654179A (en) 1990-11-14 1997-08-05 Hri Research, Inc. Nucleic acid preparation methods
DE4038293A1 (en) 1990-11-28 1992-06-04 Inst Molekularbiologie Ak Nucleic acid adduct for in situ hybridisation to give nucleic acid sequences - comprises water soluble basic polymer and nucleic acid sequence formed by polymerase chain reaction and/or reverse transcriptase reaction
US5204246A (en) * 1990-12-26 1993-04-20 Pioneer Hi-Bred International, Inc. Dna isolation method
US5296347A (en) * 1991-02-08 1994-03-22 Ciba Corning Diagnostics Corp. Bridge immunoassay
US5491223A (en) * 1991-02-28 1996-02-13 Daicel Chemical Industries, Ltd. Polysaccharide derivative and separating agent
GB9107124D0 (en) 1991-04-05 1991-05-22 Dynal As Chemical process
CA2067711C (en) 1991-05-03 2000-08-08 Daniel Lee Woodard Solid phase extraction purification of dna
US6251581B1 (en) * 1991-05-22 2001-06-26 Dade Behring Marburg Gmbh Assay method utilizing induced luminescence
US5155018A (en) 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
FR2679255B1 (en) * 1991-07-17 1993-10-22 Bio Merieux METHOD OF IMMOBILIZING A NUCLEIC FRAGMENT BY PASSIVE FIXING ON A SOLID SUPPORT, SOLID SUPPORT THUS OBTAINED AND ITS USE.
WO1993003167A1 (en) 1991-08-06 1993-02-18 The World Health Organisation Method of isolation of dna
US5329000A (en) * 1991-10-31 1994-07-12 Becton, Dickinson And Company Purification of DNA with silicon tetrahydrazide
US6051380A (en) 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
EP0555798B1 (en) 1992-02-13 1999-05-06 Becton, Dickinson and Company Hydrated celite and purification of DNA
DE4208645A1 (en) * 1992-03-18 1993-09-23 Bayer Ag OPTICAL SOLID PHASE BIOSENSOR BASED ON FLUORESCENT COLOR-MARGINED POLYIONIC LAYERS
DE4237381C1 (en) 1992-11-05 1994-04-28 Diagen Inst Molekularbio Carrier material for the simultaneous binding of genotypic and phenotypic substances
GB9212164D0 (en) * 1992-06-09 1992-07-22 Medical Res Council Preparation of nucleic acids
US5710028A (en) * 1992-07-02 1998-01-20 Eyal; Nurit Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor
GB9220773D0 (en) 1992-10-02 1992-11-18 Mini Agriculture & Fisheries Detection of antigens and nucleic acids
GB2272391A (en) 1992-11-05 1994-05-18 Gordon Henry Turner Drilling jig
GB9223334D0 (en) 1992-11-06 1992-12-23 Hybaid Ltd Magnetic solid phase supports
US5672659A (en) 1993-01-06 1997-09-30 Kinerton Limited Ionic molecular conjugates of biodegradable polyesters and bioactive polypeptides
EP0693085B1 (en) * 1993-03-29 2001-10-31 Minnesota Mining And Manufacturing Company Method of coupling ligands within porous supports (p.e. azlactone) and uses thereof
US5438129A (en) * 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using partially fluorinated aluminum hydroxide adsorbant
US5503816A (en) * 1993-09-27 1996-04-02 Becton Dickinson And Company Silicate compounds for DNA purification
GB2282138B (en) 1993-09-28 1997-09-03 Tosoh Corp Method of extracting nucleic acids and method of detecting specified nucleic acid sequences
US5919455A (en) 1993-10-27 1999-07-06 Enzon, Inc. Non-antigenic branched polymer conjugates
US20030044777A1 (en) * 1993-10-28 2003-03-06 Kenneth L. Beattie Flowthrough devices for multiple discrete binding reactions
GB9323305D0 (en) 1993-11-11 1994-01-05 Medinnova Sf Isoaltion of nucleic acid
DE4432654C2 (en) 1994-09-14 1998-03-26 Qiagen Gmbh Process for the isolation of nucleic acids from natural sources
WO1995027718A2 (en) 1994-04-08 1995-10-19 Hybridon, Inc. Purification of oligodeoxynucleotide phosphorothioates using anion exchange chromatography
AU2907995A (en) 1994-06-23 1996-01-19 Dade International Inc. Method for the rapid isolation of nucleic acid
US5797202A (en) 1994-08-25 1998-08-25 Kabushiki Kaisha Iseki Kaihatsu Koki Vertical hole excavating machine
US5622822A (en) * 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5705628A (en) * 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5652348A (en) * 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
US5660984A (en) 1994-12-09 1997-08-26 Davis; Thomas E. DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US5631146A (en) * 1995-01-19 1997-05-20 The General Hospital Corporation DNA aptamers and catalysts that bind adenosine or adenosine-5'-phosphates and methods for isolation thereof
US5762903A (en) 1995-03-10 1998-06-09 Korea Atomic Energy Research Institute Radioactive chitosan complex for radiation therapy
WO1996033998A1 (en) * 1995-04-28 1996-10-31 Daiichi Pharmaceutical Co., Ltd. Pentacyclic compounds
US5702147A (en) * 1995-05-09 1997-12-30 Bestop, Inc. Tailgate sealing arrangement
AR003122A1 (en) 1995-05-19 1998-07-08 Merck & Co Inc A PROCESS FOR ISOLATION AND PURIFICATION OF PLASMIDS IN LARGE SCALE FERMENTATORS AND ISOLATED AND PURIFIED DNA OBTAINED THROUGH SUCH A PROCESS.
WO1996041810A1 (en) 1995-06-08 1996-12-27 Progen Industries Limited Method and apparatus for dna extraction
DE19520398B4 (en) 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetic pigment
JP2965131B2 (en) 1995-07-07 1999-10-18 東洋紡績株式会社 Magnetic carrier for nucleic acid binding and nucleic acid isolation method using the same
DE69612390T2 (en) * 1995-08-28 2001-10-31 Advanced Nano Technologies Pty METHOD FOR PRODUCING ULTRAFINE PARTICLES
US5783686A (en) 1995-09-15 1998-07-21 Beckman Instruments, Inc. Method for purifying nucleic acids from heterogenous mixtures
EP0866802A1 (en) 1995-11-21 1998-09-30 Novartis AG Multivalent polymers, processes for their preparation, and their use for preparing biologically active compounds.
US5612473A (en) * 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
US6803188B1 (en) 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US5981735A (en) 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
SE9600590D0 (en) 1996-02-19 1996-02-19 Pharmacia Biotech Ab Methods for chromatographic separation of peptides and nucleic acid and new high-affinity ion exchange matrix
WO1997031105A1 (en) 1996-02-25 1997-08-28 Precision System Science Co., Ltd. Method for treating biopolymers, microorganisms or materials by using more than one type of magnetic particles
NO961221D0 (en) 1996-03-26 1996-03-26 Oeystein Fodstad Method of identifying new genes associated with site-preferential cancer metastasis formation
SE9601318D0 (en) * 1996-04-04 1996-04-04 Pharmacia Biosensor Ab Method for nucleic acid analysis
US5780319A (en) * 1996-04-19 1998-07-14 Pasteur Sanofi Diagnostics Immunoassays to detect antiphospholipid antibodies
US5916746A (en) * 1996-05-09 1999-06-29 Kirkegaard & Perry Laboratories, Inc. Formazan-based immunoassay
US5962412A (en) 1996-06-10 1999-10-05 Arqule, Inc. Method of making polymers having specific properties
US5981235A (en) 1996-07-29 1999-11-09 Promega Corporation Methods for isolating nucleic acids using alkaline protease
JP3793287B2 (en) 1996-08-22 2006-07-05 ペンタックス株式会社 Method for isolating plasmid DNA
CA2214495C (en) 1996-09-25 2002-02-05 Daniel L. Woodard Hydrated zirconium silicate composition for purification of nucleic acids
US5795748A (en) 1996-09-26 1998-08-18 Becton Dickinson And Company DNA microwell device and method
EP0938549A1 (en) * 1996-10-10 1999-09-01 Biotechnology Research And Development Corporation Polymer-protein composites and methods for their preparation and use
ATE259056T1 (en) 1996-11-06 2004-02-15 Sequenom Inc METHOD FOR MASS SPECTROMETRY
US6060246A (en) * 1996-11-15 2000-05-09 Avi Biopharma, Inc. Reagent and method for isolation and detection of selected nucleic acid sequences
EP0853123A1 (en) 1997-01-10 1998-07-15 Roche Diagnostics GmbH Purification of DNA by 'cross-flow-filtration'
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
US5770712A (en) * 1997-03-14 1998-06-23 Virginia Tech Intellectual Properties, Inc. Crosslinked hydrogel beads from chitosan
GB9709728D0 (en) * 1997-05-13 1997-07-02 Dynal As Single step method
US20040152084A1 (en) 2003-01-31 2004-08-05 Slattum Paul M. Compounds and processes for single-pot attachment of a label to nucleic acid
US5958788A (en) 1997-05-28 1999-09-28 Nalco Chemical Company Luminol tagged polymers for treatment of industrial systems
WO1998059076A1 (en) * 1997-06-25 1998-12-30 Promega Corporation Method of isolating rna
EP0897978A3 (en) 1997-08-22 2001-10-17 Becton, Dickinson and Company Zirconium oxide and related compounds for purification of nucleic acids
DE19739218A1 (en) 1997-09-08 1999-03-11 Boehringer Mannheim Gmbh Purification of substances from a biological sample
JP4304348B2 (en) * 1997-09-22 2009-07-29 独立行政法人理化学研究所 DNA isolation method
DE19746874A1 (en) 1997-10-23 1999-04-29 Qiagen Gmbh Isolation of nucleic acids
CA2305995A1 (en) 1997-10-23 1999-05-06 Nicholas A.D. Burke Labelling of polymers and sequencing of nucleic acids
DE19747572C1 (en) 1997-10-28 1999-04-08 Inst Chemo Biosensorik Apparatus for fluorescence immunoassay
US6120985A (en) 1997-10-31 2000-09-19 Bbi Bioseq, Inc. Pressure-enhanced extraction and purification
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
KR20010032806A (en) * 1997-12-06 2001-04-25 매튜 존 베이커 Isolation of nucleic acids
JP2002503814A (en) * 1998-02-12 2002-02-05 イムニベスト・コーポレイション Methods and reagents for rapid and efficient isolation of circulating cancer cells
FR2777355B1 (en) 1998-04-10 2000-05-12 Bio Merieux PROCESS FOR FIXING A BIOLOGICAL MOLECULE ON THE SURFACE OF A SUPPORT CONSISTING OF SILICA OR METAL OXIDE
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
US6194562B1 (en) * 1998-04-22 2001-02-27 Promega Corporation Endotoxin reduction in nucleic acid purification
US6287772B1 (en) * 1998-04-29 2001-09-11 Boston Probes, Inc. Methods, kits and compositions for detecting and quantitating target sequences
US6534262B1 (en) * 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
US6287765B1 (en) 1998-05-20 2001-09-11 Molecular Machines, Inc. Methods for detecting and identifying single molecules
US6245507B1 (en) * 1998-08-18 2001-06-12 Orchid Biosciences, Inc. In-line complete hyperspectral fluorescent imaging of nucleic acid molecules
WO2000029848A1 (en) 1998-11-17 2000-05-25 Proteo Tools Separation, screening, and identification of biological targets
ATE317297T1 (en) 1998-11-23 2006-02-15 Us Gov Sec Army CLEANING METHOD AND APPARATUS
FI990082A0 (en) 1999-01-18 1999-01-18 Labsystems Oy Purification process using magnetic particles
WO2000044928A2 (en) 1999-01-27 2000-08-03 Halaka Folim G Materials and methods for the purification of polyelectrolytes
CA2360042A1 (en) 1999-01-29 2000-08-03 Amersham Pharmacia Biotech K.K. Temperature-responsive polymer compound and process for producing the same
DE19907023A1 (en) 1999-02-19 2000-08-24 Bayer Ag Isolation of nucleic acids, useful for e.g. genetic diagnosis by amplification, by adsorption onto polymeric beads at neutral or acidic pH then release at basic pH
GB9907245D0 (en) 1999-03-29 1999-05-26 Goldsborough Andrew Cleavage of nucleic acids from solid supports
AU4058100A (en) 1999-04-09 2000-11-14 Arcturus Engineering, Inc. Generic cdna or protein array for customized assays
CA2270106C (en) 1999-04-23 2006-03-14 Yousef Haj-Ahmad Nucleic acid purification and process
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
ATE530648T1 (en) 1999-05-14 2011-11-15 Promega Corp CELL CONCENTRATION AND LYSATE CLARIFICATION USING PARAMAGNETIC PARTICLES
US6472141B2 (en) 1999-05-21 2002-10-29 Caliper Technologies Corp. Kinase assays using polycations
US6645733B1 (en) 1999-06-25 2003-11-11 Ingeneus Corporation Fluorescent intensity method for assaying binding between proteins or peptides
ATE320443T1 (en) * 1999-07-15 2006-04-15 Qiagen Gmbh METHOD FOR SEPARATING PARTICLE SUBSTRATES FROM A SOLUTION BY MINIMIZING PARTICLE LOSS
WO2001038585A2 (en) 1999-11-24 2001-05-31 The Regents Of The University Of California Polymer arrays and methods of using labeled probe molecules to identify and quantify target molecule expression
US6582907B1 (en) * 1999-12-09 2003-06-24 Pharmacia & Upjohn Company Use of fluorescence correlation spectroscopy to identify compounds that bind to target species under isothermal denaturing conditions
US6638906B1 (en) * 1999-12-13 2003-10-28 Nobex Corporation Amphiphilic polymers and polypeptide conjugates comprising same
US7078536B2 (en) 2001-03-14 2006-07-18 Genesoft Pharmaceuticals, Inc. Charged compounds comprising a nucleic acid binding moiety and uses therefor
US6489160B2 (en) 2000-03-16 2002-12-03 Kabushiki Kaisha Toshiba Method for producing nucleic acid strand immobilized carrier
US7262006B1 (en) 2000-05-01 2007-08-28 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of DNA from sample without using cell lysing reagent
GB0013658D0 (en) 2000-06-05 2000-07-26 Dynal Asa Nucleic acid isolation
US7501284B2 (en) 2000-07-31 2009-03-10 Applera Corporation Apparatus and method for specific release of captured extension products
WO2002016580A2 (en) 2000-08-24 2002-02-28 Bio-Rad Laboratories, Inc. Flow-through system for lysate clarification and nucleic acid binding in a single step
US6627748B1 (en) 2000-09-11 2003-09-30 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
GB0026560D0 (en) * 2000-10-30 2000-12-13 Novartis Ag Organic compounds
EP1207207B1 (en) 2000-11-20 2004-10-27 Sony International (Europe) GmbH Process for immobilization of nucleic acid molecules on a substrate
US6743586B2 (en) * 2001-01-29 2004-06-01 The United States Of America As Represented By The Secretary Of The Commerce Rapid fluorescence detection of binding to nucleic acid drug targets labelled with highly fluorescent nucleotide base analogs
JP3975319B2 (en) 2001-03-02 2007-09-12 富士ゼロックス株式会社 Developing device and image forming apparatus using the same
CA2439448C (en) * 2001-03-02 2012-05-22 University Of Western Ontario Polymer precursors of radiolabeled compounds, and methods of making and using the same
US20040259082A1 (en) 2001-04-24 2004-12-23 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymers
US7338805B2 (en) * 2001-05-04 2008-03-04 Bio Merieux Labeling reagents, methods for synthesizing such reagents and methods for detecting biological molecules
US7183116B2 (en) * 2001-05-14 2007-02-27 The Institute For Systems Biology Methods for isolation and labeling of sample molecules
AU2002332637A1 (en) * 2001-08-22 2003-03-10 Watson Pharmaceuticals, Inc. Conjugates targeted to target receptors
US20030099954A1 (en) * 2001-11-26 2003-05-29 Stefan Miltenyi Apparatus and method for modification of magnetically immobilized biomolecules
US20030229013A1 (en) 2001-12-07 2003-12-11 Shih-Kwang Wu Solid phase method for synthesis peptide-spacer-lipid conjugates, conjugates synthesized thereby and targeted liposomes containing the same
DE60225788T2 (en) * 2001-12-27 2008-07-17 Matsushita Electric Industrial Co., Ltd., Kadoma IMMUNO REACTION MEASUREMENT PROCEDURE FOR IMMUNE REACTION MEASUREMENT
US20030147958A1 (en) 2002-01-29 2003-08-07 Cheol-Hee Ahn Biodegradable multi-block copolymers of poly(amino acid)s and poly(ethylene glycol) for the delivery of bioactive agents
US20040009506A1 (en) * 2002-03-29 2004-01-15 Genentech, Inc. Methods and compositions for detection and quantitation of nucleic acid analytes
GB0209539D0 (en) * 2002-04-26 2002-06-05 Avecia Ltd Monomer Polymer and process
GB0212826D0 (en) 2002-05-31 2002-07-10 Dna Res Innovations Ltd Materials and methods relating to polyions and substance delivery
CN102766695A (en) 2002-06-20 2012-11-07 加州大学评议会 Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
ATE400650T1 (en) 2002-10-18 2008-07-15 Sloning Biotechnology Gmbh METHOD FOR PRODUCING NUCLEIC ACID MOLECULES
FR2847581B1 (en) * 2002-11-21 2007-03-23 Commissariat Energie Atomique METHOD FOR FIXING A PROTEIN ON A POLYMER BASED ON PYRROLE AND ITS USE IN THE MANUFACTURE OF A SENSOR
GB0229287D0 (en) 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
US20050123932A1 (en) * 2003-12-09 2005-06-09 Mekbib Astatke Nucleic acid-chelating agent conjugates
BE1016163A6 (en) 2004-08-18 2006-04-04 Mc Anton Sa METHOD Percutaneous HYPERBARIC OXYGEN SPRAY fluorocarbons.

Also Published As

Publication number Publication date
US20140291576A1 (en) 2014-10-02
EP0796327B1 (en) 2004-07-28
US20040215011A1 (en) 2004-10-28
US20080300396A1 (en) 2008-12-04
US20090149646A1 (en) 2009-06-11
CA2207608C (en) 2009-04-07
US20070190559A1 (en) 2007-08-16
US7173124B2 (en) 2007-02-06
AU4182996A (en) 1996-07-03
JP3787354B2 (en) 2006-06-21
US8691969B2 (en) 2014-04-08
WO1996018731A3 (en) 1996-09-12
US20090068724A1 (en) 2009-03-12
US20110251382A1 (en) 2011-10-13
DE69533317T2 (en) 2005-12-01
AU706211B2 (en) 1999-06-10
DE69533317D1 (en) 2004-09-02
ATE272110T1 (en) 2004-08-15
WO1996018731A2 (en) 1996-06-20
US7989614B2 (en) 2011-08-02
GB9425138D0 (en) 1995-02-08
US20060058519A1 (en) 2006-03-16
JPH11501504A (en) 1999-02-09
EP0796327A2 (en) 1997-09-24

Similar Documents

Publication Publication Date Title
CA2207608A1 (en) Isolation of nucleic acid
AU600997B2 (en) Method of isolating and purifying nucleic acids from biological samples
AU2331095A (en) Method and apparatus for fully automated nucleic acid amplification, nucleic acid assay and immunoassay
CA2289943A1 (en) Solid-phase nucleic acid isolation
WO2001071732A3 (en) Porous ferro- or ferrimagnetic glass particles for isolating molecules
EP1865068A3 (en) Cell isolation method
CA2286573A1 (en) Nucleic acid archiving
DE69731939D1 (en) ISOLATION OF SINGLE-STRUCTURED NUCLEIC ACIDS
CA2212185A1 (en) Methods for the isothermal amplification of nucleic acid molecules
AU2929495A (en) Nucleic acid sequence amplification method
EP1027145A4 (en) Apparatuses and methods for isolating nucleic acid
EP0261956A3 (en) Process for rapid isolation of high molecular weight dna
AU2253397A (en) Nucleic acid analysis techniques
AU3376897A (en) Microfluidic method for nucleic acid amplification
AU7225591A (en) Nucleic acid enzymes for cleaving dna
AU3027699A (en) Nucleic acid analysis method
CA2209080A1 (en) Fluorometric assay for detecting nucleic acid cleavage
WO1999067619A3 (en) Compounds and methods for detecting biomolecules
AU2907995A (en) Method for the rapid isolation of nucleic acid
EP1679383A3 (en) Method and device for purifying nucleid acids
AU3595493A (en) Method for nucleic acid hybridization using single-stranded DNA binding protein
AU1989197A (en) Automated nucleic acid extraction from samples
CA2263713A1 (en) Lyophilization of cultured human cells to preserve rna and dna
AU1686395A (en) (deltex) proteins, nucleic acids, and antibodies, and related methods and compositions
WO2001024937A3 (en) Separation apparatus and method

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20141212