CA2125174A1 - Novel polypeptides for promoting cell attachment - Google Patents

Novel polypeptides for promoting cell attachment

Info

Publication number
CA2125174A1
CA2125174A1 CA002125174A CA2125174A CA2125174A1 CA 2125174 A1 CA2125174 A1 CA 2125174A1 CA 002125174 A CA002125174 A CA 002125174A CA 2125174 A CA2125174 A CA 2125174A CA 2125174 A1 CA2125174 A1 CA 2125174A1
Authority
CA
Canada
Prior art keywords
fibronectin
novel polypeptides
promoting cell
cell attachment
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002125174A
Other languages
French (fr)
Other versions
CA2125174C (en
Inventor
Mark H. Ginsberg
Edward F. Plow
Ronald Bowditch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scripps Research Institute
Original Assignee
Mark H. Ginsberg
Edward F. Plow
Ronald Bowditch
The Scripps Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mark H. Ginsberg, Edward F. Plow, Ronald Bowditch, The Scripps Research Institute filed Critical Mark H. Ginsberg
Publication of CA2125174A1 publication Critical patent/CA2125174A1/en
Application granted granted Critical
Publication of CA2125174C publication Critical patent/CA2125174C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/75—Fibrinogen
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Hematology (AREA)
  • Veterinary Medicine (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Transplantation (AREA)
  • Dermatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Diabetes (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Novel polypeptides derived from human fibronectin, and fusion proteins containing those peptide sequences are describes which define a receptor binding site on fibronectin that binds to the platelet receptor glycoprotein GPIIb-IIIa expressed by cells.
The receptor binding site of human fibronectin includes at least fibronectin amino acid residues 1410-1436. The polypeptides facilitate attachment of cells to substrates either alone or in conjunction with RGD-containing peptides. Vectors preparing the fusion proteins and antibodies are also described. Methods for promoting cell attachment and for inhibiting cell adhesion are also described.
CA002125174A 1991-12-05 1992-12-04 Novel polypeptides for promoting cell attachment Expired - Lifetime CA2125174C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US804,224 1977-06-07
US07/804,224 US5492890A (en) 1990-12-03 1991-12-05 Polypeptides for promoting cell attachment
PCT/US1992/010511 WO1993011229A1 (en) 1991-12-05 1992-12-04 Novel polypeptides for promoting cell attachment

Publications (2)

Publication Number Publication Date
CA2125174A1 true CA2125174A1 (en) 1993-06-10
CA2125174C CA2125174C (en) 2007-04-10

Family

ID=25188472

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002125174A Expired - Lifetime CA2125174C (en) 1991-12-05 1992-12-04 Novel polypeptides for promoting cell attachment

Country Status (12)

Country Link
US (1) US5492890A (en)
EP (1) EP0619839B1 (en)
JP (2) JP4272257B2 (en)
AT (1) ATE194382T1 (en)
AU (1) AU680607B2 (en)
CA (1) CA2125174C (en)
DE (1) DE69231228T2 (en)
DK (1) DK0619839T3 (en)
ES (1) ES2147558T3 (en)
GR (1) GR3034352T3 (en)
PT (1) PT619839E (en)
WO (1) WO1993011229A1 (en)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5770198A (en) * 1988-05-18 1998-06-23 The Research Foundation Of The State Of New York Platelet-specific chimeric 7E3 immunoglobulin
US5780303A (en) * 1990-04-06 1998-07-14 La Jolla Cancer Research Foundation Method and composition for treating thrombosis
US6521594B1 (en) 1990-04-06 2003-02-18 La Jolla Cancer Research Foundation Method and composition for treating thrombosis
US5672585A (en) * 1990-04-06 1997-09-30 La Jolla Cancer Research Foundation Method and composition for treating thrombosis
US6017877A (en) * 1990-04-06 2000-01-25 La Jolla Cancer Research Foundation Method and composition for treating thrombosis
US5645815A (en) * 1991-02-08 1997-07-08 Diatide, Inc. Radiolabled compounds for thrombus imaging
US5629291A (en) * 1992-01-31 1997-05-13 La Jolla Cancer Research Foundation Methods of modulating fibronectin extracellular matrix assembly
US5968476A (en) * 1992-05-21 1999-10-19 Diatide, Inc. Technetium-99m labeled peptides for thrombus imaging
US7083979B1 (en) * 1994-03-25 2006-08-01 Indiana University Foundation Methods for enhanced retroviral-mediated gene transfer
US5763270A (en) * 1995-06-07 1998-06-09 Genemedicine, Inc. Plasmid for delivery of nucleic acids to cells and methods of use
WO1997011604A1 (en) * 1995-09-29 1997-04-03 Indiana University Foundation Methods for enhanced virus-mediated dna transfer using molecules with virus- and cell-binding domains
DE69636122T2 (en) * 1995-11-13 2006-12-07 Takara Bio Inc., Otsu PROCEDURE FOR TRANSFERRING TO TARGET CELLS WITH A RETROVIRUS
US6242587B1 (en) 1996-10-02 2001-06-05 The University Of North Carolina At Chapel Hill DNA molecules encoding a calcium-integrin binding protein
US5840514A (en) * 1996-11-21 1998-11-24 The Regents Of The University Of Michigan Methods of testing cancer and anticancer drugs
US6025150A (en) * 1996-11-21 2000-02-15 The Regents Of The University Of Michigan Methods and compositions for wound healing
ATE433495T1 (en) * 1996-11-21 2009-06-15 Univ Michigan INVASION INHIBITORS FOR USE IN WOUND HEALING AND CANCER TREATMENT
US6001965A (en) 1996-11-21 1999-12-14 The Regents Of The University Of Michigan Anticancer compounds and methods
US6331409B1 (en) 1996-11-21 2001-12-18 The Regents Of The University Of Michigan Methods and compositions for wound healing
WO1999047070A1 (en) * 1998-03-18 1999-09-23 Wake Forest University Improved implantable biomaterials, compositions and methods for their preparation and uses thereof
EP1108015A1 (en) * 1998-08-25 2001-06-20 Board Of Regents Of The University Of Washington Streptavidin mutants having secondary functional domains
US20050271701A1 (en) * 2000-03-15 2005-12-08 Orbus Medical Technologies, Inc. Progenitor endothelial cell capturing with a drug eluting implantable medical device
US20160287708A9 (en) * 2000-03-15 2016-10-06 Orbusneich Medical, Inc. Progenitor Endothelial Cell Capturing with a Drug Eluting Implantable Medical Device
CA2400319C (en) * 2000-03-15 2008-09-16 Orbus Medical Technologies Inc. Coating that promotes endothelial cell adherence
US8460367B2 (en) * 2000-03-15 2013-06-11 Orbusneich Medical, Inc. Progenitor endothelial cell capturing with a drug eluting implantable medical device
US8088060B2 (en) * 2000-03-15 2012-01-03 Orbusneich Medical, Inc. Progenitor endothelial cell capturing with a drug eluting implantable medical device
US9522217B2 (en) * 2000-03-15 2016-12-20 Orbusneich Medical, Inc. Medical device with coating for capturing genetically-altered cells and methods for using same
US6893851B2 (en) 2000-11-08 2005-05-17 Surface Logix, Inc. Method for arraying biomolecules and for monitoring cell motility in real-time
US7326563B2 (en) 2000-11-08 2008-02-05 Surface Logix, Inc. Device and method for monitoring leukocyte migration
US7374906B2 (en) 2000-11-08 2008-05-20 Surface Logix, Inc. Biological assays using gradients formed in microfluidic systems
US6699665B1 (en) * 2000-11-08 2004-03-02 Surface Logix, Inc. Multiple array system for integrating bioarrays
US7033821B2 (en) * 2000-11-08 2006-04-25 Surface Logix, Inc. Device for monitoring cell motility in real-time
US6864065B2 (en) * 2000-11-08 2005-03-08 Surface Logix, Inc. Assays for monitoring cell motility in real-time
US6844184B2 (en) * 2000-11-08 2005-01-18 Surface Logix, Inc. Device for arraying biomolecules and for monitoring cell motility in real-time
US7033819B2 (en) 2000-11-08 2006-04-25 Surface Logix, Inc. System for monitoring cell motility in real-time
JP4590117B2 (en) * 2001-02-21 2010-12-01 森永製菓株式会社 Immunoassay reagent
US20030082148A1 (en) * 2001-10-31 2003-05-01 Florian Ludwig Methods and device compositions for the recruitment of cells to blood contacting surfaces in vivo
US20060078535A1 (en) * 2004-10-13 2006-04-13 The Regents Of The University Of Michigan Anticancer compounds and methods
US8921516B2 (en) * 2010-12-08 2014-12-30 Corning Incorporated Synthetic, defined fibronectin mimetic peptides and surfaces modified with the same
CN111454350B (en) * 2020-06-01 2020-12-29 广东丸美生物技术股份有限公司 Recombinant fibronectin mutant and application thereof

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3382031D1 (en) * 1982-08-04 1991-01-10 Jolla Cancer Res Found Polypeptide.
US4792525A (en) * 1982-08-04 1988-12-20 La Jolla Cancer Research Foundation Tetrapeptide
US4578079A (en) * 1982-08-04 1986-03-25 La Jolla Cancer Research Foundation Tetrapeptide
US4980279A (en) * 1986-04-15 1990-12-25 Scripps Clinic And Research Foundation Cellular fibronectin: polypeptides, anti-polypeptide antibodies and assay methods
US5019646A (en) * 1987-08-25 1991-05-28 Regents Of The University Of Minnesota Polypeptides with fibronectin activity
JPH0829098B2 (en) * 1988-01-05 1996-03-27 寳酒造株式会社 Cell adhesion activity polypeptide
US5773574A (en) * 1990-12-03 1998-06-30 The Scripps Research Institute Polypeptides for promoting cell attachment
AU1515392A (en) * 1991-04-26 1992-10-29 Mark Rossi Method and apparatus for cane trash removal
US5453489A (en) * 1992-01-31 1995-09-26 La Jolla Cancer Research Foundation Polypeptide fragments of fibronectin which can modulate extracellular matrix assembly

Also Published As

Publication number Publication date
GR3034352T3 (en) 2000-12-29
AU680607B2 (en) 1997-08-07
EP0619839A4 (en) 1995-09-20
EP0619839A1 (en) 1994-10-19
DE69231228D1 (en) 2000-08-10
US5492890A (en) 1996-02-20
DK0619839T3 (en) 2000-09-18
ES2147558T3 (en) 2000-09-16
JP2007312788A (en) 2007-12-06
AU3240893A (en) 1993-06-28
CA2125174C (en) 2007-04-10
ATE194382T1 (en) 2000-07-15
WO1993011229A1 (en) 1993-06-10
PT619839E (en) 2000-10-31
JP4272257B2 (en) 2009-06-03
JPH07504812A (en) 1995-06-01
EP0619839B1 (en) 2000-07-05
DE69231228T2 (en) 2000-11-30

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Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20121204