CA2054602A1 - Method for assaying for a substance that affects an sh2-phosphorylated ligand regulatory system - Google Patents
Method for assaying for a substance that affects an sh2-phosphorylated ligand regulatory systemInfo
- Publication number
- CA2054602A1 CA2054602A1 CA002054602A CA2054602A CA2054602A1 CA 2054602 A1 CA2054602 A1 CA 2054602A1 CA 002054602 A CA002054602 A CA 002054602A CA 2054602 A CA2054602 A CA 2054602A CA 2054602 A1 CA2054602 A1 CA 2054602A1
- Authority
- CA
- Canada
- Prior art keywords
- phosphorylated ligand
- domain
- phosphorylated
- subdomain
- ligand
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
- C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
- C07K14/4706—Guanosine triphosphatase activating protein, GAP
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/71—Assays involving receptors, cell surface antigens or cell surface determinants for growth factors; for growth regulators
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- G01N2333/91205—Phosphotransferases in general
- G01N2333/9121—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/20—Screening for compounds of potential therapeutic value cell-free systems
Abstract
A method for assaying a medium for the presence of a substance that affects an SH2-phosphorylated ligand regulatory system. The method employs an SH2-like domain or a subdomain thereof and a phosphorylated ligand. The phosphophorylated ligand is capable of interacting with the SH2-like domain or a subdomain thereof to form an SH2-phosphorylated ligand complex. The SH2-like domain or subdomain and/or the phosphorylated ligand are present in a known concentration. The SH2-like domain or a subdomain thereof and the phosphorylated ligand are incubated with a substance which is suspected of affecting an SH2-phosphorylated ligand regulatory system. The method is carried out under conditions which permit the formation of the SH2-phosphorylated ligand complex. SH2-phosphorylated ligand complex, free SH2-like domain or subdomains thereof, or non-complexed phosphorylated ligand are assayed. The invention also relates to an isolated SH2-phosphorylated ligand complex; a method of using an isolated SH2-like domain or a subdomain thereof to screen for phosphorylated ligands which are active in an SH2-phosphorylated ligand regulatory system; a method of using an isolated SH2-like domain or a subdomain thereof to regulate the interaction of a signalling protein with a related phosphorylated ligand; and a pharmaceutical composition comprising an isolated SH2-like domain or a subdomain thereof for use as an agonist or antagonist of the interaction of a signalling protein with a related phosphorylated ligand.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/786,057 US5352660A (en) | 1991-10-31 | 1991-10-31 | Method for assaying for a substance that affects a SH2-phosphorylated ligand regulatory system |
CA002054602A CA2054602C (en) | 1991-10-31 | 1991-10-31 | Method for assaying for a substance that affects an sh2-phosphorylated ligand regulatory system |
CA002417838A CA2417838A1 (en) | 1991-10-31 | 1991-10-31 | Method for assaying for a substance that affects an sh-2 phosphorylated ligand regulatory system |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/786,057 US5352660A (en) | 1991-10-31 | 1991-10-31 | Method for assaying for a substance that affects a SH2-phosphorylated ligand regulatory system |
CA002054602A CA2054602C (en) | 1991-10-31 | 1991-10-31 | Method for assaying for a substance that affects an sh2-phosphorylated ligand regulatory system |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002417838A Division CA2417838A1 (en) | 1991-10-31 | 1991-10-31 | Method for assaying for a substance that affects an sh-2 phosphorylated ligand regulatory system |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2054602A1 true CA2054602A1 (en) | 1993-05-01 |
CA2054602C CA2054602C (en) | 2003-04-22 |
Family
ID=25674846
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002054602A Expired - Fee Related CA2054602C (en) | 1991-10-31 | 1991-10-31 | Method for assaying for a substance that affects an sh2-phosphorylated ligand regulatory system |
Country Status (2)
Country | Link |
---|---|
US (1) | US5352660A (en) |
CA (1) | CA2054602C (en) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994007913A1 (en) * | 1992-09-25 | 1994-04-14 | Warner-Lambert Company | Peptide antagonists of sh2 binding and therapeutic uses thereof |
WO1995002057A1 (en) * | 1993-07-09 | 1995-01-19 | The Institute Of Cancer Research | Protein tyrosine kinase and ligands thereof |
WO1995031545A2 (en) * | 1994-05-13 | 1995-11-23 | Children's Hospital Of Los Angeles | Diagnostics and treatments for cancers expressing tyrosine phosphorylated crkl protein |
EP0750630A1 (en) * | 1994-03-11 | 1997-01-02 | Ariad Pharmaceuticals, Inc. | Methods and materials for identifying inhibitors of molecular interactions mediated by sh3 domains |
WO1997032020A2 (en) * | 1996-02-29 | 1997-09-04 | Mount Sinai Hospital Corporation | Shc proteins |
EP0804218A1 (en) * | 1994-03-17 | 1997-11-05 | National Jewish Center For Immunology And Respiratory Medicine | Product and process for regulating signal transduction pathways |
WO1998057177A1 (en) * | 1997-06-09 | 1998-12-17 | Regeneron Pharmaceuticals, Inc. | Assay systems for leptin-enhancing agents |
Families Citing this family (52)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU652576B2 (en) | 1990-09-13 | 1994-09-01 | Duke University | Expression of G protein coupled receptors in yeast |
CA2100860C (en) * | 1991-01-18 | 2003-11-25 | Joseph Schlessinger | A receptor tyrosine kinase target protein cdna cloning method and hgrb proteins |
US5776716A (en) * | 1994-02-01 | 1998-07-07 | Terrapin Technologies, Inc. | Methods for identifying agents which block the interaction of fyn with PKC-theta, and uses thereof |
US5783405A (en) * | 1994-02-01 | 1998-07-21 | Terrapin Technologies, Inc. | Rapid screening method for effectors of signal transduction |
US5935803A (en) * | 1994-02-01 | 1999-08-10 | Terrapin Technologies, Inc. | Methods to identify immunomodulators using cognate interaction of PKC-theta |
US6262023B1 (en) | 1994-02-01 | 2001-07-17 | The Board Of Trustees Of The Leland Stanford Junior University | WD-40 derived peptides and uses thereof |
US5519003A (en) | 1994-02-01 | 1996-05-21 | Board Of Trustees Of The Leland Stanford Junior University | WD-40-derived peptides and uses thereof |
US6423684B1 (en) | 1994-02-01 | 2002-07-23 | The Board Of Trustees Of The Leland Stanford Junior University | WD-40 derived peptides and uses thereof |
US6037134A (en) * | 1994-03-07 | 2000-03-14 | New York University Medical Center | Methods that detect compounds that disrupt receptor tyrosine kinase/GRB-7 complexes |
DE69532127T2 (en) * | 1994-07-20 | 2004-08-26 | Genetics Institute, Inc., Cambridge | INTERACTION FILLING SYSTEMS FOR DETECTING PROTEIN INTERACTIONS |
US6399296B1 (en) * | 1994-07-20 | 2002-06-04 | The General Hospital Corporation | Interaction trap systems for detecting protein interactions |
US5786454A (en) * | 1994-09-16 | 1998-07-28 | Washington University School Of Medicine | Modified SH2 domains |
US5744313A (en) * | 1994-12-09 | 1998-04-28 | The Regents Of The University Of California | Assay employing novel protein domain which binds tyrosine phosphorylated proteins |
US5659012A (en) * | 1995-03-10 | 1997-08-19 | Whitehead Institute For Biomedical Research | Peptide which binds SH2 domains of protein tyrosine phosphatase SH-PTP1 |
AU711141B2 (en) * | 1995-04-07 | 1999-10-07 | Cytogen Corporation | Polypeptides having a functional domain of interest and methods of identifying and using same |
US6280964B1 (en) * | 1995-04-14 | 2001-08-28 | The Regents Of The University Of California | Binding sites for phosphotyrosine binding domains |
AU6405596A (en) * | 1995-06-30 | 1997-02-05 | Smithkline Beecham Corporation | Use of stat 5 sh2 domain specific compounds to enhance erythropoiesis |
AU6480796A (en) * | 1995-06-30 | 1997-02-05 | Smithkline Beecham Corporation | Use of stat 6 sh2 domain specific compounds to treat allergic reactions |
EP0871648A1 (en) * | 1995-09-15 | 1998-10-21 | Merck & Co., Inc. | A high throughput assay using fusion proteins |
US5776696A (en) * | 1995-09-15 | 1998-07-07 | Merck & Co., Inc. | High throughput assay using fusion proteins |
US5783398A (en) * | 1995-09-15 | 1998-07-21 | Merck & Co., Inc. | High throughput assay using fusion proteins |
US6028053A (en) * | 1995-10-27 | 2000-02-22 | Mount Sinai Hospital Corporation | Peptide inhibitors of a phosphotyrosine-binding domain containing protein |
CA2235756A1 (en) * | 1995-10-27 | 1997-05-01 | Mount Sinai Hospital Corporation | Peptide inhibitors of a phosphotyrosine-binding domain containing protein |
JP2000506007A (en) * | 1996-02-23 | 2000-05-23 | アリアド・ファーマシューティカルズ・インコーポレイテッド | Cell-based assays |
WO1997039326A2 (en) * | 1996-04-18 | 1997-10-23 | Ariad Pharmaceuticals, Inc. | In vitro fluorescence polarization assay |
US6207397B1 (en) | 1996-04-18 | 2001-03-27 | Ariad Pharmaceuticals, Inc. | In vitro fluorescence polarization assay |
US6054286A (en) * | 1996-06-18 | 2000-04-25 | Telik, Inc. | Methods to identify immunomodulators using cognate interaction of PKC-theta |
US5912138A (en) * | 1996-07-25 | 1999-06-15 | Cold Spring Harbor Laboratory | Substrate trapping protein tyrosine phosphatases |
US20030036106A1 (en) * | 1996-10-28 | 2003-02-20 | Burke Thomas J. | Kinase and phosphatase activity measurement using fluorescence polarization |
WO1998021585A1 (en) | 1996-11-15 | 1998-05-22 | Cornell Research Foundation, Inc. | Activated ras interaction assay |
ATE271130T1 (en) | 1997-02-07 | 2004-07-15 | Univ Princeton | MODIFIED PROTEIN KINASES THAT CAN USE MODIFIED NUCLEOTIDE TRIPHOSPHATE SUBSTRATES |
US20030096760A1 (en) * | 1997-03-10 | 2003-05-22 | Smithklinebeecham Corporation | Method of antagonizing the human SRC SH2 domain |
WO1998041866A1 (en) * | 1997-03-14 | 1998-09-24 | Merck & Co., Inc. | A high throughput assay using fusion proteins |
US7541151B2 (en) | 1997-06-05 | 2009-06-02 | Duke University | Single-cell biosensor for the measurement of GPCR ligands in a test sample |
US5891646A (en) * | 1997-06-05 | 1999-04-06 | Duke University | Methods of assaying receptor activity and constructs useful in such methods |
US6528271B1 (en) | 1997-06-05 | 2003-03-04 | Duke University | Inhibition of βarrestin mediated effects prolongs and potentiates opioid receptor-mediated analgesia |
US6440386B1 (en) | 1998-07-06 | 2002-08-27 | Immunomedics, Inc. | Stabilized radiophosphate-labeled proteins |
US6355786B1 (en) * | 1998-10-30 | 2002-03-12 | Vanderbilt University | Purified and isolated protein zero related (PZR) and therapeutic and screening methods using same |
US6610483B1 (en) * | 1999-07-23 | 2003-08-26 | Princeton University | Methods for identifying cellular responses attributable to signaling molecule inhibition and inhibitors thereof |
US6165728A (en) * | 1999-11-19 | 2000-12-26 | Isis Pharmaceuticals Inc. | Antisense modulation of NCK-2 expression |
EP1377828A4 (en) * | 2001-04-10 | 2006-11-15 | Childrens Medical Center | Methods of analysis and labeling of protein-protein interactions |
US20030166502A1 (en) * | 2001-05-10 | 2003-09-04 | Washington University | Differential regulation of T cell survival and proliferation |
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US20030182669A1 (en) * | 2002-03-19 | 2003-09-25 | Rockman Howard A. | Phosphoinositide 3-kinase mediated inhibition of GPCRs |
US7290215B2 (en) * | 2002-06-03 | 2007-10-30 | Microsoft Corporation | Dynamic wizard interface system and method |
TW200418829A (en) * | 2003-02-14 | 2004-10-01 | Avanir Pharmaceutics | Inhibitors of macrophage migration inhibitory factor and methods for identifying the same |
WO2005021546A1 (en) * | 2003-08-22 | 2005-03-10 | Avanir Pharmaceuticals | Substituted naphthyridine derivatives as inhibitors of macrophage migration inhibitory factor and their use in the treatment of human diseases |
EP1861407A1 (en) * | 2005-03-24 | 2007-12-05 | Avanir Pharmaceuticals | Thienopyridinone derivatives as macrophage migration inhibitory factor inhibitors |
US8703769B2 (en) * | 2005-07-15 | 2014-04-22 | The University Of North Carolina At Chapel Hill | Use of EGFR inhibitors to prevent or treat obesity |
GB0605689D0 (en) * | 2006-03-21 | 2006-05-03 | Novartis Ag | Organic compounds |
JP2013537410A (en) | 2010-07-23 | 2013-10-03 | シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニー | Genome editing using targeted endonucleases and single-stranded nucleic acids |
CN117517041B (en) * | 2024-01-08 | 2024-04-02 | 嘉华药锐生物科技(昆山)有限公司 | Method for improving enrichment efficiency of tyrosine phosphorylated peptide and application thereof |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5100661A (en) * | 1988-03-21 | 1992-03-31 | Eye Research Institute Of Retina Foundation | Method for regulating cellular signal transducing system |
-
1991
- 1991-10-31 CA CA002054602A patent/CA2054602C/en not_active Expired - Fee Related
- 1991-10-31 US US07/786,057 patent/US5352660A/en not_active Expired - Fee Related
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994007913A1 (en) * | 1992-09-25 | 1994-04-14 | Warner-Lambert Company | Peptide antagonists of sh2 binding and therapeutic uses thereof |
WO1995002057A1 (en) * | 1993-07-09 | 1995-01-19 | The Institute Of Cancer Research | Protein tyrosine kinase and ligands thereof |
EP0750630A4 (en) * | 1994-03-11 | 1998-01-07 | Ariad Pharma Inc | Methods and materials for identifying inhibitors of molecular interactions mediated by sh3 domains |
EP0750630A1 (en) * | 1994-03-11 | 1997-01-02 | Ariad Pharmaceuticals, Inc. | Methods and materials for identifying inhibitors of molecular interactions mediated by sh3 domains |
EP0804218A4 (en) * | 1994-03-17 | 1997-11-12 | ||
EP0804218A1 (en) * | 1994-03-17 | 1997-11-05 | National Jewish Center For Immunology And Respiratory Medicine | Product and process for regulating signal transduction pathways |
US5667981A (en) * | 1994-05-13 | 1997-09-16 | Childrens Hospital Of Los Angeles | Diagnostics and treatments for cancers expressing tyrosine phosphorylated CRKL protein |
WO1995031545A3 (en) * | 1994-05-13 | 1996-08-01 | Los Angeles Childrens Hospital | Diagnostics and treatments for cancers expressing tyrosine phosphorylated crkl protein |
WO1995031545A2 (en) * | 1994-05-13 | 1995-11-23 | Children's Hospital Of Los Angeles | Diagnostics and treatments for cancers expressing tyrosine phosphorylated crkl protein |
WO1997032020A2 (en) * | 1996-02-29 | 1997-09-04 | Mount Sinai Hospital Corporation | Shc proteins |
WO1997032020A3 (en) * | 1996-02-29 | 1997-11-13 | Mount Sinai Hospital Corp | Shc proteins |
US6077686A (en) * | 1996-02-29 | 2000-06-20 | Mount Sinai Hospital Corporation | Shc proteins |
WO1998057177A1 (en) * | 1997-06-09 | 1998-12-17 | Regeneron Pharmaceuticals, Inc. | Assay systems for leptin-enhancing agents |
Also Published As
Publication number | Publication date |
---|---|
US5352660A (en) | 1994-10-04 |
CA2054602C (en) | 2003-04-22 |
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Legal Events
Date | Code | Title | Description |
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EEER | Examination request | ||
MKLA | Lapsed |