CA2048015C - Washing cells - Google Patents
Washing cellsInfo
- Publication number
- CA2048015C CA2048015C CA002048015A CA2048015A CA2048015C CA 2048015 C CA2048015 C CA 2048015C CA 002048015 A CA002048015 A CA 002048015A CA 2048015 A CA2048015 A CA 2048015A CA 2048015 C CA2048015 C CA 2048015C
- Authority
- CA
- Canada
- Prior art keywords
- red blood
- wash solution
- centrifuging
- layer
- blood cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3692—Washing or rinsing blood or blood constituents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
- A61M1/3696—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2202/00—Special media to be introduced, removed or treated
- A61M2202/04—Liquids
- A61M2202/0413—Blood
- A61M2202/0429—Red blood cells; Erythrocytes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
- B04B2005/0464—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation with hollow or massive core in centrifuge bowl
Abstract
Red blood cells being salvaged are centrifuged into a liquid layer, mixed with a wash solution, and there-after separated therefrom.
Description
`- %o48o~ 5 74424-41 WASHING CLLLS
Fleld of the Inventlon This lnvention relates to washing cells, and in particular to washing red blood cells that have been formed in a centrlfuge lnto a fluld layer.
Background of the Inventlon It ls known to salvage an lndivldual's blood by collectlng it with suction, centrlfuglng lt to form a layer of red blood cells, washlng under centrlfugatlon the layer with saline to remove plasma and lmpurities such as heparin, and returning it to the same individual (i.e., an "autologous"
salvage~. A centrifuge bowl useful in such salvage is disclosed in Feldman et al. U.S. Pat. No. 4,684,361, "Centrifuge", granted Aug. 4, 1987; a tubing set useful in such salvage and lncludlng a centrifuge bowl generally in accordance with Feldman et al.
has been sold by Cobe Laboratories, Inc., assignee hereof, under the trademark BRAT.
Summary of the Inventlon I have discovered that lmproved washlng of cells results lf they are lntermedlately ln centrlfugatlon brought together ln dlspersed form wlth a volume of wash llquld.
In a broad aspect, the lnventlon resides in the method of removing impurities from red blood cells which comprises centrifuging blood containing said impurities to form a red blood cell layer also containing said impurities, stopping said centrifuging, mixlng red blood cells from sald layer wlth a wash solutlon r~
-la 74424-41 during sald stopplng, and centrlfuglng the resultant mlxture to remove sald wash solutlon.
In preferred embodlments, a centrlfuged layer of red blood cells ls formed, ls washed under centrlfugatlon wlth a sallne solutlon to remove plasma and other centrlfuged-out materlals, ls transferred to a contalner wlth a volume of wash sallne greater than the red blood cell layer volume, ls agltated therewith, ls retransferred as a mlxture to centrlfuge the wash llquld therefrom, ls glven a further sallne wash under centrlfugatlon, and ls then transferred to a relnfuslon bag.
20~8~1S
.` ~ ~
Preferred Embodiment Disclosure of the presently preferred embodiment of the invention follows:
Drawings The figure is a partially diagrammatic view of -~ an apparatus for practicing the invention.
Structure There is shown in the figure a centrifuge bowl 140, in a tubing set, indicated generally at 12 (not to scale), useful in the invention.
Centrifuge bowl 140 is a part of tubing set 12.
A tubing set as disclosed, as well as equipment in which to mount and use it in a prior art method as well as the method of the present invention, is sold by applicant's assignee, Cobe Laboratories, Inc., under the trademark BRAT. Tubing set 12 includes manifold 14, which communicates with blood collection reservoir line 22, saline bag line 24, and blood reinfusion bag line 26. Manifold 14 is connected by tubing 28 with centrifuge inlet 122. Valving 30, 32, 34 for selec-tively opening and closing tubing 22, 24, and 26 is carried by the cooperating equipment above referred to, in which the centrifugation set 12 is mounted in use.
Set 12 also includes waste collection bag 36, which is supplied through outlet 128 of centrifuge 110, and which is hung in use on the above-mentioned equipment; this equipment also rotatively drivès bowl 140. Valves 50, 52 are slide clamps, as well known in the art.
. .
,' ~ ~
The set 12 does not as supplied actually include reservoir 38 and saline bag 42; ancl reinfusion bag 44 is not connected up as supplied, although included in the set package.
Method In the presently preferred embodiment, bowl 140, which holds about 250 ml. in its portions not occupied by spacer 150 and inlet downspout 14Z, is spun empty on the BRAT equipment above referred to to a rotational velocity of 4400 rpm, following which blood to be salvaged is introduced from a blood collection reservoir 38 (rigid polvcarbonate, of 3-liter volume, into which is moved blood that has been salvaged from the patient and processed as known in the art) carried by the equipment above referred to, through valve 30, line 22, manifold 14, peristaltic pump 40, and lines 28 into inlet 122, and bowl 140 until a fluid red blood cell layer forms on the inner surface of bowl 140 to a thickness such that there is left adjacent the outer periphery of spacer 150 a gap about l/8 inch in radial distance. In the preferred embodiment gap size is monitored automatically using a reflective color sensor to sense red blood cell layer surface location. During this period plasma and impurities such as heparin and hemoglobin emerge into bag 36 through centrifuge bowl outlet 128. Depending on the hematocrit of the blood in the collection reservoir feeding the centri-fuge, for example, 700 ml. of blood is introduced into the centrifuge, to produce a roughly 150 ml. layer of fluid, pre-dominately red blood cells, with most of the remaining volume of centrifuged blood going into bag 36.
Fleld of the Inventlon This lnvention relates to washing cells, and in particular to washing red blood cells that have been formed in a centrlfuge lnto a fluld layer.
Background of the Inventlon It ls known to salvage an lndivldual's blood by collectlng it with suction, centrlfuglng lt to form a layer of red blood cells, washlng under centrlfugatlon the layer with saline to remove plasma and lmpurities such as heparin, and returning it to the same individual (i.e., an "autologous"
salvage~. A centrifuge bowl useful in such salvage is disclosed in Feldman et al. U.S. Pat. No. 4,684,361, "Centrifuge", granted Aug. 4, 1987; a tubing set useful in such salvage and lncludlng a centrifuge bowl generally in accordance with Feldman et al.
has been sold by Cobe Laboratories, Inc., assignee hereof, under the trademark BRAT.
Summary of the Inventlon I have discovered that lmproved washlng of cells results lf they are lntermedlately ln centrlfugatlon brought together ln dlspersed form wlth a volume of wash llquld.
In a broad aspect, the lnventlon resides in the method of removing impurities from red blood cells which comprises centrifuging blood containing said impurities to form a red blood cell layer also containing said impurities, stopping said centrifuging, mixlng red blood cells from sald layer wlth a wash solutlon r~
-la 74424-41 during sald stopplng, and centrlfuglng the resultant mlxture to remove sald wash solutlon.
In preferred embodlments, a centrlfuged layer of red blood cells ls formed, ls washed under centrlfugatlon wlth a sallne solutlon to remove plasma and other centrlfuged-out materlals, ls transferred to a contalner wlth a volume of wash sallne greater than the red blood cell layer volume, ls agltated therewith, ls retransferred as a mlxture to centrlfuge the wash llquld therefrom, ls glven a further sallne wash under centrlfugatlon, and ls then transferred to a relnfuslon bag.
20~8~1S
.` ~ ~
Preferred Embodiment Disclosure of the presently preferred embodiment of the invention follows:
Drawings The figure is a partially diagrammatic view of -~ an apparatus for practicing the invention.
Structure There is shown in the figure a centrifuge bowl 140, in a tubing set, indicated generally at 12 (not to scale), useful in the invention.
Centrifuge bowl 140 is a part of tubing set 12.
A tubing set as disclosed, as well as equipment in which to mount and use it in a prior art method as well as the method of the present invention, is sold by applicant's assignee, Cobe Laboratories, Inc., under the trademark BRAT. Tubing set 12 includes manifold 14, which communicates with blood collection reservoir line 22, saline bag line 24, and blood reinfusion bag line 26. Manifold 14 is connected by tubing 28 with centrifuge inlet 122. Valving 30, 32, 34 for selec-tively opening and closing tubing 22, 24, and 26 is carried by the cooperating equipment above referred to, in which the centrifugation set 12 is mounted in use.
Set 12 also includes waste collection bag 36, which is supplied through outlet 128 of centrifuge 110, and which is hung in use on the above-mentioned equipment; this equipment also rotatively drivès bowl 140. Valves 50, 52 are slide clamps, as well known in the art.
. .
,' ~ ~
The set 12 does not as supplied actually include reservoir 38 and saline bag 42; ancl reinfusion bag 44 is not connected up as supplied, although included in the set package.
Method In the presently preferred embodiment, bowl 140, which holds about 250 ml. in its portions not occupied by spacer 150 and inlet downspout 14Z, is spun empty on the BRAT equipment above referred to to a rotational velocity of 4400 rpm, following which blood to be salvaged is introduced from a blood collection reservoir 38 (rigid polvcarbonate, of 3-liter volume, into which is moved blood that has been salvaged from the patient and processed as known in the art) carried by the equipment above referred to, through valve 30, line 22, manifold 14, peristaltic pump 40, and lines 28 into inlet 122, and bowl 140 until a fluid red blood cell layer forms on the inner surface of bowl 140 to a thickness such that there is left adjacent the outer periphery of spacer 150 a gap about l/8 inch in radial distance. In the preferred embodiment gap size is monitored automatically using a reflective color sensor to sense red blood cell layer surface location. During this period plasma and impurities such as heparin and hemoglobin emerge into bag 36 through centrifuge bowl outlet 128. Depending on the hematocrit of the blood in the collection reservoir feeding the centri-fuge, for example, 700 ml. of blood is introduced into the centrifuge, to produce a roughly 150 ml. layer of fluid, pre-dominately red blood cells, with most of the remaining volume of centrifuged blood going into bag 36.
- 2 0 g 8 0 1 5 The red blood cell layer is then washed with 200 ml. of "saline" (in the preferred embodiment a 0.9% solu-tion in water of NaCl), which is pumped through line 24 (valve 32 controlling flow therethrough, being now open, and that controlling flow through line 22 being now closed), manifold 14, line 28, pump 40, and inlet 122, from saline bag 42 carried by the above-mentioned equipment, and which has a volume capacity of 1000 ml., but which contains before pumping from it the 200 ml. wash portion just mentioned only 800 ml. of saline, pumping and washing being done with the bowl still spinning at full speed, some of the wash liquid effluent moving on then into bag 36.
The reversible-direction pump 40 carried by the above-mentioned equipment cooperates with line 28 to selec-tively pump in either direction therethrough.
Centrifuge bowl 140 is then stopped, the pumping direction of pump 40 reversed, and the fluid red blood cell material, no longer a layer upon stopping of the centrifuge, but descending into the bottom of bowl 140, and which has a volume of about 150 ml., is pumped, along with about 100 ml.
of saline, still in the bowl, through line 28, manifold 14, line 24, and valve 32 into saline bag 42, at a rate causing turbulence, for mixing. Agit~ation for mixing there is further provided by pumping in, following the red blood cells, 100 ml. of air.
Bowl 140 is then rotated again at 4400 rpm, and the entire contents of saline bag 42 introduced thereinto, pump 40 pumping direction having been reversed again. A new red blood cell Iiquid laver results, and excess, lighter, fluid again goes off into bag 36.
The now-empty saline bag 42 is replaced by a fresh saline bag 42 containing a full 1000 ml. of saline. With 204~0 ~ 5 the bowl stlll splnnlng, a further 200 ml. wash for the red blood cell layer exterlor ls provlded, effluent movlng lnto bag 36.
Valve 32 ls then closed; centrlfuge bowl 140 rotatlon ls then stopped; valve 34 ls then opened; and the pumplng dlrectlon of pump 40 agaln reversed, and the red blood cell layer dropped ln the bowl and pumped through manlfold 14, llne 26, and valve 34 lnto relnfuslon bag 44, whlch may then be glven to a medlcal attendant for re-lntroductlon lnto the patlent.
The centrlfugatlon set 12 may lf deslred be used for multlple sequences accordlng to the lnventlon. Waste bag 36 may sultably have a volume of 10 llters, and serve for several sequences. That bag or relnfuslon bag 44 (volume 1 llter ln the preferred embodlment) may be replaced lf deslred, as ls preferably, as seen, sallne bag 4Z. The tublng ln the preferred embodlment ls preferably ~ lnch ID, except that the tublng to and from waste bag 36 downstream of valve 50 ls preferably sllghtly larger ln ID.
The method of the lnventlon provldes blood of lmproved freedom from undeslred lmpurltles, as compared wlth prlor art methods of attemptlng to purlfy centrlfuged layers of red blood cells.
Other Embodlments Other embodlments of the lnventlon wlll occur to those skllled in the art. Thus, ~ust as examples, salvage need not be autologous. The lntermedlate uncentrlfuged wash step need not be done ln the sallne bag. Radlal gap ad~acent the red blood cell layer surface may be regulated manually.
X
The reversible-direction pump 40 carried by the above-mentioned equipment cooperates with line 28 to selec-tively pump in either direction therethrough.
Centrifuge bowl 140 is then stopped, the pumping direction of pump 40 reversed, and the fluid red blood cell material, no longer a layer upon stopping of the centrifuge, but descending into the bottom of bowl 140, and which has a volume of about 150 ml., is pumped, along with about 100 ml.
of saline, still in the bowl, through line 28, manifold 14, line 24, and valve 32 into saline bag 42, at a rate causing turbulence, for mixing. Agit~ation for mixing there is further provided by pumping in, following the red blood cells, 100 ml. of air.
Bowl 140 is then rotated again at 4400 rpm, and the entire contents of saline bag 42 introduced thereinto, pump 40 pumping direction having been reversed again. A new red blood cell Iiquid laver results, and excess, lighter, fluid again goes off into bag 36.
The now-empty saline bag 42 is replaced by a fresh saline bag 42 containing a full 1000 ml. of saline. With 204~0 ~ 5 the bowl stlll splnnlng, a further 200 ml. wash for the red blood cell layer exterlor ls provlded, effluent movlng lnto bag 36.
Valve 32 ls then closed; centrlfuge bowl 140 rotatlon ls then stopped; valve 34 ls then opened; and the pumplng dlrectlon of pump 40 agaln reversed, and the red blood cell layer dropped ln the bowl and pumped through manlfold 14, llne 26, and valve 34 lnto relnfuslon bag 44, whlch may then be glven to a medlcal attendant for re-lntroductlon lnto the patlent.
The centrlfugatlon set 12 may lf deslred be used for multlple sequences accordlng to the lnventlon. Waste bag 36 may sultably have a volume of 10 llters, and serve for several sequences. That bag or relnfuslon bag 44 (volume 1 llter ln the preferred embodlment) may be replaced lf deslred, as ls preferably, as seen, sallne bag 4Z. The tublng ln the preferred embodlment ls preferably ~ lnch ID, except that the tublng to and from waste bag 36 downstream of valve 50 ls preferably sllghtly larger ln ID.
The method of the lnventlon provldes blood of lmproved freedom from undeslred lmpurltles, as compared wlth prlor art methods of attemptlng to purlfy centrlfuged layers of red blood cells.
Other Embodlments Other embodlments of the lnventlon wlll occur to those skllled in the art. Thus, ~ust as examples, salvage need not be autologous. The lntermedlate uncentrlfuged wash step need not be done ln the sallne bag. Radlal gap ad~acent the red blood cell layer surface may be regulated manually.
X
Claims (9)
1. The method of removing impurities from red blood cells which comprises:
centrifuging blood containing said impurities to form a red blood cell layer also containing said impurities, stopping said centrifuging, mixing red blood cells from said layer with a wash solution during said stopping, and centrifuging the resultant mixture to remove said wash solution.
centrifuging blood containing said impurities to form a red blood cell layer also containing said impurities, stopping said centrifuging, mixing red blood cells from said layer with a wash solution during said stopping, and centrifuging the resultant mixture to remove said wash solution.
2. The method of claim 1 which includes the further step of washing said layer with a wash solution prior to said stopping said centrifuging.
3. The method of claim 1 which includes the further step of washing a second red blood cell layer resulting from said centrifuging the resulting mixture with a wash solution while centrifuging said second red blood cell layer.
4. The method of claim 1 in which the volume of said wash solution is in excess of the volume of said red blood cell layer.
5. The method of claim 4 in which the volume of said wash solution is four times the volume of said red blood cell layer.
6. The method of claim 1 which includes the further steps of washing said layer with a wash solution prior to said stopping said centrifuging and washing a second red blood cell layer resulting from said centrifuging the resulting mixture with a wash solution while centrifuging said second red blood cell layer, the volume of each said wash solution being in excess of the volume of each said red blood cell layer.
7. The method of claim 1 in which said wash solution is a dilute solution of sodium chloride and in which said mixing step includes moving said red blood cells from said layer into a container for said wash solution.
8. The method of claim 7 in which said mixing step includes agitation of said cells relative to said wash solution.
9. The method of claim 1 in which said red blood cells of said layer are removed from a centrifuge bowl and thereafter mixed with said wash solution.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07609748 US5141486B1 (en) | 1990-11-05 | 1990-11-05 | Washing cells |
| US609,748 | 1990-11-05 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2048015A1 CA2048015A1 (en) | 1992-05-06 |
| CA2048015C true CA2048015C (en) | 1996-03-19 |
Family
ID=24442169
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002048015A Expired - Fee Related CA2048015C (en) | 1990-11-05 | 1991-07-26 | Washing cells |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US5141486B1 (en) |
| JP (1) | JP2644120B2 (en) |
| CA (1) | CA2048015C (en) |
| DE (1) | DE4132716C2 (en) |
| FR (1) | FR2668714B1 (en) |
| GB (1) | GB2250699B (en) |
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| JP6912391B2 (en) | 2015-07-13 | 2021-08-04 | ヘモネティクス・コーポレーションＨａｅｍｏｎｅｔｉｃｓ Ｃｏｒｐｏｒａｔｉｏｎ | Systems and methods for removing fat from collected blood |
| US11541161B2 (en) | 2016-06-24 | 2023-01-03 | Haemonetics Corporation | System and method for continuous flow red blood cell washing |
| US10758652B2 (en) | 2017-05-30 | 2020-09-01 | Haemonetics Corporation | System and method for collecting plasma |
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| US11412967B2 (en) | 2018-05-21 | 2022-08-16 | Fenwal, Inc. | Systems and methods for plasma collection |
| JP6976458B2 (en) | 2018-05-21 | 2021-12-08 | フェンウォール、インコーポレイテッド | Systems and methods for optimizing plasma collection |
| US10683478B1 (en) * | 2019-05-16 | 2020-06-16 | Shenzhen Eureka biotechnology Co. Ltd | Device and system for processing a liquid sample containing cells |
| US11957998B2 (en) * | 2019-06-06 | 2024-04-16 | Pneumatic Scale Corporation | Centrifuge system for separating cells in suspension |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4684361A (en) * | 1985-10-11 | 1987-08-04 | Cardiovascular Systems, Inc. | Centrifuge |
| US4216770A (en) * | 1979-02-09 | 1980-08-12 | Baxter Travenol Laboratories, Inc. | Sickle cell therapeutic treatment |
| JPS5628540A (en) * | 1979-08-15 | 1981-03-20 | Nec Corp | Level monitor system of frequency multiplex terminal station unit |
| JPS6219177A (en) * | 1985-07-17 | 1987-01-27 | 株式会社クラレ | Blood treating apparatus |
| FR2587495B1 (en) * | 1985-09-18 | 1988-01-15 | Rgl Transfusion Sanguine Centr | MEDIUM AND METHOD FOR SEPARATING MONONUCLEATED CELLS FROM BLOOD AND BONE MARROW |
| SE8601891D0 (en) * | 1986-04-24 | 1986-04-24 | Svante Jonsson | PLASMA SWITCH TREATMENT AND TROMBOCYTING MACHINE |
| US4668214A (en) * | 1986-06-09 | 1987-05-26 | Electromedics, Inc. | Method of washing red blood cells |
| WO1989001792A1 (en) * | 1987-08-28 | 1989-03-09 | Haemonetics Corporation | Method and apparatus for cell washing |
| JPH01230521A (en) * | 1989-01-13 | 1989-09-14 | Nippon Sekijiyuujishiya | Separation of blood component |
-
1990
- 1990-11-05 US US07609748 patent/US5141486B1/en not_active Expired - Lifetime
-
1991
- 1991-07-26 CA CA002048015A patent/CA2048015C/en not_active Expired - Fee Related
- 1991-07-26 FR FR9109522A patent/FR2668714B1/en not_active Expired - Fee Related
- 1991-09-20 JP JP3241815A patent/JP2644120B2/en not_active Expired - Lifetime
- 1991-10-01 DE DE4132716A patent/DE4132716C2/en not_active Expired - Lifetime
- 1991-11-05 GB GB9123610A patent/GB2250699B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| DE4132716A1 (en) | 1992-05-14 |
| US5141486A (en) | 1992-08-25 |
| GB9123610D0 (en) | 1992-01-02 |
| GB2250699B (en) | 1995-02-08 |
| US5141486B1 (en) | 1996-01-30 |
| FR2668714B1 (en) | 1993-02-12 |
| CA2048015A1 (en) | 1992-05-06 |
| DE4132716C2 (en) | 1994-11-10 |
| JP2644120B2 (en) | 1997-08-25 |
| JPH04246363A (en) | 1992-09-02 |
| FR2668714A1 (en) | 1992-05-07 |
| GB2250699A (en) | 1992-06-17 |
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Legal Events
| Date | Code | Title | Description |
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| EEER | Examination request | ||
| MKLA | Lapsed |