CA2006549A1 - Procede de recombinaison in vivo de sequences d'adn partiellement homologues - Google Patents

Procede de recombinaison in vivo de sequences d'adn partiellement homologues

Info

Publication number
CA2006549A1
CA2006549A1 CA2006549A CA2006549A CA2006549A1 CA 2006549 A1 CA2006549 A1 CA 2006549A1 CA 2006549 A CA2006549 A CA 2006549A CA 2006549 A CA2006549 A CA 2006549A CA 2006549 A1 CA2006549 A1 CA 2006549A1
Authority
CA
Canada
Prior art keywords
dna sequences
homologous dna
partially homologous
recombination
vivo recombination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2006549A
Other languages
English (en)
Other versions
CA2006549C (fr
Inventor
Miroslav Radman
Christiane Rayssiguier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mixis France SA
Original Assignee
Miroslav Radman
Christiane Rayssiguier
Setratech
Mixis France
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miroslav Radman, Christiane Rayssiguier, Setratech, Mixis France filed Critical Miroslav Radman
Publication of CA2006549A1 publication Critical patent/CA2006549A1/fr
Application granted granted Critical
Publication of CA2006549C publication Critical patent/CA2006549C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Abstract

La présente invention concerne un procédé de recombinaison in vivo de séquences d'ADN partiellement homologues présentant jusqu'à
30 % de mésappariements de bases. Selon sa caractéristique essentielle, on met en présence lesdites séquences dans des cellules ou un organisme dont le système enzymatique de réparation des mésappariements est défectueux ou a été inactivé transitoirement par saturation le temps d'obtenir la recombinaison entre lesdites séquences d'ADN ou tout autre mutant qui augmente la recombinaison intergénérique.
CA002006549A 1988-12-26 1989-12-22 Procede de recombinaison in vivo de sequences d'adn partiellement homologues Expired - Lifetime CA2006549C (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8817185 1988-12-26
FR8817185A FR2641793B1 (fr) 1988-12-26 1988-12-26 Procede de recombinaison in vivo de sequences d'adn presentant des mesappariements de bases

Publications (2)

Publication Number Publication Date
CA2006549A1 true CA2006549A1 (fr) 1990-06-26
CA2006549C CA2006549C (fr) 2000-09-05

Family

ID=9373422

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002006549A Expired - Lifetime CA2006549C (fr) 1988-12-26 1989-12-22 Procede de recombinaison in vivo de sequences d'adn partiellement homologues

Country Status (13)

Country Link
US (2) US5912119A (fr)
EP (1) EP0449923B1 (fr)
JP (1) JP3056524B2 (fr)
AT (1) ATE127519T1 (fr)
AU (1) AU4834390A (fr)
CA (1) CA2006549C (fr)
DE (1) DE68924174T2 (fr)
ES (1) ES2077058T3 (fr)
FR (1) FR2641793B1 (fr)
IE (1) IE72469B1 (fr)
IL (1) IL92856A (fr)
WO (1) WO1990007576A1 (fr)
ZA (1) ZA899902B (fr)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20060257890A1 (en) * 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5837458A (en) * 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
AU4784996A (en) * 1995-07-26 1997-02-26 Netherlands Cancer Institute, The Homologous recombination in mismatch repair inactivated eukaryotic cells
US6489145B1 (en) 1996-07-09 2002-12-03 Diversa Corporation Method of DNA shuffling
US20020028443A1 (en) * 1999-09-27 2002-03-07 Jay M. Short Method of dna shuffling with polynucleotides produced by blocking or interrupting a synthesis or amplification process
JP2000502568A (ja) * 1996-01-10 2000-03-07 ノボ ノルディスク アクティーゼルスカブ 細胞内での突然変異ライブラリーの ｉｎ ｖｉｖｏ製造のための方法
US6165718A (en) 1996-01-10 2000-12-26 Novo Nordisk A/S Novo Alle Method for in vivo production of a mutant library in cells
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US7115413B2 (en) 1996-04-01 2006-10-03 Mixis France S.A. Meiotic recombination in vivo of partially homologous DNA sequences
US6242211B1 (en) 1996-04-24 2001-06-05 Terragen Discovery, Inc. Methods for generating and screening novel metabolic pathways
US20070009930A1 (en) * 1996-12-18 2007-01-11 Maxygen, Inc. Methods and compositions for polypeptide engineering
WO1998028416A1 (fr) * 1996-12-20 1998-07-02 Novo Nordisk A/S Recombinaison in vivo
JP4062366B2 (ja) 1997-01-17 2008-03-19 マキシジェン，インコーポレイテッド 再帰的配列組換えによる全細胞および生物の進化
US6265201B1 (en) 1997-01-17 2001-07-24 Regents Of The University Of Minnesota DNA molecules and protein displaying improved triazine compound degrading ability
US7148054B2 (en) * 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
AUPO974597A0 (en) * 1997-10-10 1997-11-06 Rhone-Poulenc Agro Methods for obtaining plant varieties
DK1036198T3 (da) 1997-12-08 2013-01-02 California Inst Of Techn Fremgangsmåde til fremstilling af polynukleotid- og polypeptidsekvenser
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
US6846655B1 (en) * 1998-06-29 2005-01-25 Phylos, Inc. Methods for generating highly diverse libraries
US6438561B1 (en) * 1998-11-19 2002-08-20 Navigation Technologies Corp. Method and system for using real-time traffic broadcasts with navigation systems
US6917882B2 (en) * 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6376246B1 (en) * 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
WO2000042559A1 (fr) * 1999-01-18 2000-07-20 Maxygen, Inc. Procedes de chargement de structures de donnees utilisees dans des simulations evolutives
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US20030054390A1 (en) * 1999-01-19 2003-03-20 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
AU3210200A (en) 1999-01-19 2000-08-01 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
US20070065838A1 (en) * 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7026155B2 (en) 1999-02-02 2006-04-11 Regents Of The University Of California Method of reducing bacterial proliferation
US6518042B1 (en) 1999-02-24 2003-02-11 Novozymes A/S Process for making DNA libraries in filamentous fungal cells using a novel cloned gene involved in the mismatch repair system of filamentous fungal cells
AU2536400A (en) * 1999-02-24 2000-09-14 Novozymes A/S Fungal cells with inactivated dna mismatch repair system
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US20020088021A1 (en) * 2000-09-18 2002-07-04 Mahajan Pramod B. Rice MLH1 ortholog and uses thereof
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
WO2003062435A1 (fr) * 2002-01-18 2003-07-31 Morphotek, Inc. Methode de generation de cellules genetiquement modifiees pour la regulation et l'analyse de genes specifiques d'un locus
DK1333095T3 (da) 2002-01-31 2006-07-17 Mixis France Sa Fremgangsmåde til reversibel, funktionel inaktivering af fejlparringsreparationssystemet
EP1501927A2 (fr) * 2002-05-02 2005-02-02 University Of North Carolina At Chapel Hill Mutagenese in vitro, phenotypage et cartographie genetique
EP1763579A1 (fr) * 2004-07-06 2007-03-21 Mixis France S.A. Generation de genes de recombinaison dans des bacteriophages
EP1734125A1 (fr) * 2005-06-16 2006-12-20 Institut National De La Recherche Agronomique Recombinaison homéologuée dans les plantes ou leurs cellules, où MSH2 a été inactivé
US20080124355A1 (en) * 2006-09-22 2008-05-29 David Gordon Bermudes Live bacterial vaccines for viral infection prophylaxis or treatment
WO2010111690A2 (fr) 2009-03-27 2010-09-30 Life Technologies Corporation Compositions de polymérase et procédés
PT2478100E (pt) 2010-04-09 2014-02-20 Eviagenics S A Método de geração de mosaicos de genes
DK2677881T3 (da) 2011-02-24 2020-07-13 Erber Ag Enzymer til behandling af foder
JP2014519831A (ja) 2011-06-21 2014-08-21 エヴィアジェニクス ソシエテ・アノニム 代謝的進化の方法
CN104105793A (zh) 2011-10-12 2014-10-15 埃微杰尼克斯有限公司 在真核细胞中产生基因嵌合体的方法
BR112015015594B1 (pt) 2012-12-27 2022-07-05 Rhodia Operations Célula de levedura, método de produção de uma célula de levedura, uso de uma célula de levedura e método de biossíntese heteróloga de um valinoide
EP2749644B1 (fr) 2012-12-27 2018-08-22 Rhodia Operations Cellule hôte recombinante pour la production biosynthétique de la vanilline
US9481903B2 (en) 2013-03-13 2016-11-01 Roche Molecular Systems, Inc. Systems and methods for detection of cells using engineered transduction particles
RU2661101C2 (ru) 2013-03-13 2018-07-11 Дженевив Биосайнсис, Инк. Нерепликативные трансдукторные частицы и основанные на трансдукторных частицах репортерные системы
US9540675B2 (en) 2013-10-29 2017-01-10 GeneWeave Biosciences, Inc. Reagent cartridge and methods for detection of cells
US10351893B2 (en) 2015-10-05 2019-07-16 GeneWeave Biosciences, Inc. Reagent cartridge for detection of cells
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4863727A (en) * 1986-04-09 1989-09-05 Cetus Corporation Combination therapy using interleukin-2 and tumor necrosis factor
DE3724625A1 (de) * 1987-07-24 1989-02-02 Boehringer Mannheim Gmbh Enzymatisch inaktive, immunologisch aktive ss-galactosidase-muteine

Also Published As

Publication number Publication date
IL92856A (en) 2001-04-30
DE68924174D1 (de) 1995-10-12
IE894182L (en) 1990-06-26
ATE127519T1 (de) 1995-09-15
US5965415A (en) 1999-10-12
FR2641793B1 (fr) 1993-10-01
IE72469B1 (en) 1997-04-09
US5912119A (en) 1999-06-15
WO1990007576A1 (fr) 1990-07-12
AU4834390A (en) 1990-08-01
ES2077058T3 (es) 1995-11-16
JP3056524B2 (ja) 2000-06-26
CA2006549C (fr) 2000-09-05
ZA899902B (en) 1990-09-26
FR2641793A1 (fr) 1990-07-20
EP0449923A1 (fr) 1991-10-09
JPH04503601A (ja) 1992-07-02
IL92856A0 (en) 1990-09-17
DE68924174T2 (de) 1996-03-14
EP0449923B1 (fr) 1995-09-06

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