CA1300006C - Self-contained immunoassay element - Google Patents

Self-contained immunoassay element

Info

Publication number
CA1300006C
CA1300006C CA000560295A CA560295A CA1300006C CA 1300006 C CA1300006 C CA 1300006C CA 000560295 A CA000560295 A CA 000560295A CA 560295 A CA560295 A CA 560295A CA 1300006 C CA1300006 C CA 1300006C
Authority
CA
Canada
Prior art keywords
reagent
capillary
labelled
sample
analyte
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA000560295A
Other languages
French (fr)
Inventor
Gerd Grenner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Behring Diagnostics Inc
Original Assignee
PB Diagnostic Sytems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PB Diagnostic Sytems Inc filed Critical PB Diagnostic Sytems Inc
Application granted granted Critical
Publication of CA1300006C publication Critical patent/CA1300006C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/528Atypical element structures, e.g. gloves, rods, tampons, toilet paper
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302Apparatus specially adapted for immunological test procedures
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0605Metering of fluids
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0681Filter
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/069Absorbents; Gels to retain a fluid
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0816Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406Moving fluids with specific forces or mechanical means specific forces capillary forces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00Chemistry: analytical and immunological testing
    • Y10S436/807Apparatus included in process claim, e.g. physical support structures

Abstract

SELF CONTAINED IMMUNOASSAY ELEMENT

ABSTRACT DISCLOSURE

An element for analysis of a liquid sample including a capillary containing a fixed reagent in fluid communication with reagent reservoirs and a waste liquid reservoir. A sample introducing means is provided which permits analyte in a sample to interact with the fixed reagent. A labelled reagent is provided which interacts with either the fixed reagent or the analyte in a manner which permits detection of the concentration of analyte in the sample. The capillary, reagent reservoirs, waste liquid reservoir and the sample introducing means are arranged within a common housing so that reagent waste liquid need not be removed from the element.

Description

~3~ ~1D6 SELF CONTAINED IMMUNOASSAY ~LEMENT

BACXGROUND OF THE INVENTION
The present invention relates to a capillary device for conducting immunoassay tests. More particularly, the present invention relates to a capillary device ~or conducting an immunoassay test which device 5 contains the reagents necessary for carrying out the test and confines the reagents and sample tested within the device during the course of the test.
Immunoassay tests are used for tlle deteGt~on in body fluids of a wide variety of compositions including 10 antigens, antibodies, infectious agents and hormones. The purpose of the immunoassay test is to measure the degree of interaction between an antigen and its corresponding antibody.
It has been proposed -to utilize various wet 15 chemistry processes wherein the immunoassay interactions are conducted in tubes or plates containing multiple compartments wherein an antibody or an antigen is coated in the surface o~ the tube or compartment. The use of these devices requires a number of steps of applying and 20 then removing reagents from the compartments or tubes and requires that the volume o~ these reagents be accurately measured. In addition, relatively large samples are required to conduct the immunoassay test in th2se ~3~ 6 devices. An additional serious problem associated with these wet chemistry techniques is the disposal of biological liquids which may contain infectious agents.
European Patent Application 0,142,914 discloses a 5 self-contained microassay card which houses a wash liquid, a plurality of separated reagents and a microassay rod upon which the assay is effected. The use of the card requires the use of a syringe in order to introduce a precise volume of the sample into the chamber housing the lO microassa~ rod. This re~uires a separate step o~
measuring the precise volume desired. In addition, since the syringe is reau~red to deliver the sample under pressure, the chamber housing the microassay rod must be closed to avoid lo~s of sample which in turn re~uires a 15 precision fit plug. Since no provision is made in the device for a filter means, whole blood cannot be processed thereby re~uiring an additional step ~or separat~ng the s cellular components from the plasma components prior to initiatlng the microassay. ~astly, the device requires 20 the precise po81 tioning of pressure means at various locations on the outside surface of the device in order to effect proper se~uencing of reagents to contact sample components during the test. The reauirement of precise positioning at the proper time during the test introduces 25 a major source of error for the test.
EurGpean Patent Application 0 073,513 discloses a device for conducting assays which includes a rotor upon which are positioned a plurality of elements which house reagents in separate compartments. The compartments are 30 located within an element at different radial positions on the rotor and at least one compartment is in fluid communication with a sample application chamber located at the radially lnnermost position of the compartment. A
mixing chamber which allows for the lntroduction of a 35 liquid reagent is located within.a compartment at a radially intermediate position and a measurement chamber ~3~ 6 is located at the radially outermost position of the compartment, Liauid sample and/or liauid reagent are moved ~rom compartment to compartment and eventually to the measurement chamber by means of conduits connecting the compartments and centrifugal force caused by rotating the rotor. This mode of operation reauires that the conduits be of precise size and that the rates oP rotation be precisely controlled otherwise liauid flow into the wrong compartments occurs. These reauirements introduce a serious source of error in the assay.
P.C.T. publication number W082/02601 discloses a sol~d phase assay system which utilizes a porous solid matrlx such as treated paper or a mat of fibers. A
binding material is reacted with its antibody within the solid matrix to immobilize the reaction product within the matrix. The amount of binding material and its antibody that are reacted must be very accurately controlled using accurate volumetric micropipettes. Solutions of analyte and labelled indicator then are applied to the matrix to effect the assay either as a competitive assay or as a sandwich assay. Unbound labelled indicator is removed ~rom the matrix by a chromatographic procedure utilizing a solvent. The concentration of labelled indicator in the reaction zone then is measured. This assay is undesirable since it requires measuring precise volumes, manual washing steps and the pipetting of reagents. It is also unsuitable for as~aying whole blood.
European Patent Application 0 186 100 discloses a solid phase assay which utilizes a fiber matrix coated with a polymer capable of retaining a reagent which binds with an analyte of interest. A second reagent is bound to the bound analyte and a third reagent is introduced which, in the presence of the second reagent, produce~ a detectable response. The disadvantage of this assay is that it requires precise volumes of reagent and sample ~hich requires time-consuming volume measurement steps.

~3~

U.S. Patent 4,426,451 discloses a device for conducting an assay which utilizes two continuous capillary zones in fluid communication wi~h each other. A
; narrow passageway i8 provided between the two zones in order to temporarily stop liquid flow between the zones.
Liquid transfer between the zones is initiated by a pulse or pressure and completed by capillary action to the zone having the smaller capillary. The device i8 useful for conducting competitive assays or for separating whole blood into a cellular component and a plasma component.
Since no means are provided for at least one washing step and since separation of bound species from unbound species relies solely upon capillary action, incomplete separation of bound from unbound species occurs. This leads to error in the assay. In addition, the device does not permit conducting a sandwich assay.
It has been proposed in French Patent Application Number ~12933, published November 24, 1978 to conduct immunoassays in a capillary tube. However, no means are provided for filling the capillary and for eliminating the need for removing reagents and reaction products from the capillary. Thus, the problem of disposing infectious materials is still present.
P.C.T. publication number W086/06488 discloses a test apparatus for performing chemical, cl$nical diagnostic and like tests. The apparatus includes a housing, a sample receiving area and a plurality of rupturable containers in recesses within the housing. The containers include the predetermined amount of one of the specific reagents which are required for the test. A duct or channel connects each area with the sample receiving area where the sample is located. A member is provided in association with each container to enable the container to be ruptured when addition of the specific reagent is reauired in the test. This assay apparatus does not include a self-metering sample application element.

~L3~)~0~D6 Instead it requires an operator to pipette a precisely measured volume of the sample onto the sample receiving membrane. The membrane thickness is no~ precisely defined and therefore the apparatus is not capable of providiny very accurate quantitative results. Further, the fluicl s~ream ln the apparatus is not very well defined w1th the result that very high volumes of wash liquid, e.g., about 1-2 ml, must be used because the liquid must cover the entire test element.
It would be highly desirable to provide a means for conducting lmmunoassay tests under conditions which require only small samples, which provide accurate test results, which eliminates the need for measuring precise volumes of reagents, whlch permits the testing of whole blood and which eliminates the problems assoclated wlth dlsposlng of lnfectious liqulds.
The present invention provides a self-contained element for analysis of a liquid sample containing a component to be determined which comprises:
a housing containing a capillary tu'oe having opposed interior, top and bottom surfaces, a portion of at least one of said top and bottom surfaces having bound thereto a first reagent consistiny of either said component to be determined or the binding partner of said component to be determined, said bound first reayent defining a reaction zone and interac~ive with said component to be determined to form a first nondiffusible species, and at least one of said top and bottom surfaces including a transparent area to permit measurement of radiation from at least a portion of said reaction zone;

~' ~.;

~3i~ 6 a labelled second xeagent, said second reayent consisting of said component to be determined or ~he binding partner of said componen~ to be determined and beiny interactivè with said first nondiffusible species or said first reagent to form a second labelled species;
means for introducing at least a portion of said sample and said labelled second reagent into said caplllary tube by capillary forces;
at least one reactant reservoir containing a wash solution or a third reagent which is interactive witll said second labelled species, said at least one reactant reservoir being in fluid communication with said capillary tube;
a waste reservoir ln f.luid communication with said capillary tube adapted to receive waste fluid from said capillary tube;
and sald means for introducing sai.d sample and said labelled second reagent, said at leas~ one reactant reservoir and said waste reservoir being housed within said housing.
The invention further provides a process for analyzing for an analyte in a liquid sample within the conlines of a self~
0 contained element housing which co~prises:
introducing a liquid sample into a porous layer within said element, said porous layer containing a labelled reagent which is interactive wlth said analyte;
passing said analyte and said labelled reagen~ by capillary force ln~o a capillary having opposed top and bottom surfaces and a iirst reagent hound to at least one of said top and bottom surfaces/ said first reagent being interactive with said analyte -5a ~L31)g~

and said labelled reagent to form a first nondiffusible specles comprising said labelled reagent, said analy~e and said firs~
reagent;
washing the interior of said caplllary with a liquid composition positioned in a reservoir with:Ln said element and in fluid communication with said capillary to pass the portion of the sample noninterac~ed with said firs~ reagent into a waste reservoir located within said element, and rendering said labelled reagent detectable.
SUMMA~Y OF TH~ INVENTION
The present invention provides a self-contained immunoassay element which comprises a housing which includes one or more reservoirs containing a reagent or a wash solution used in the assay, means for intxoducing a sample into the element and a capillary tube in fluid communication with the means for introducing the sample as well as with the reagent and wash solution reservoirs. At least a portion of a surface of the capillary tube, which is the reaction zone of the element, i5 coated with an an~igen or an antibody which is interactive with khe analyte contained within the liquid sample for which the analysis is being conducted. A reservoir which is in fluid communication with the capillary is provided for spent reagent and spent wash ~solution. The element is constructed so ~hat, except for introducing the sample from an outside source lnto the element, all interactions a~ong reagents used in the assay and all wash steps are conducted within the element and waste li~uids resulting -5b-~,.`"

~3~ 1C16 from the assay are retained within the element. Each : reagent and/or wash solution is contained within a separate reservoir and is provided with means for passing the reagent or wash solut~on under pressure into the capillary.
In operation, a liguid sample containlng an analyte is introduced into the element and at least a portion o~ the sample enters the capillary under the force of capillary action and is retained therein for a sufficient time period to allow the analyte to interact with the bound first reagent (antigen or antibody~ in the capillary to form a first nondiffusible species.
labelled rea~ent positioned either in a xeservoir or in the means for introducing the sample within the element i5 introduced into the capillary either simultaneously with the analyte or subse~uently to introducing the analyte into the capillary. Since the capillary i8 filled with liquid by means of capillary action, the volume of liquid containing the analyte or a reagent in the capillary taking part in the assay is always accurately controlled.
After the desired interactions between the ~irst reagent, the labelled reagent and the analyte have been completed excess labelled reagent is removed by washing. The wash solution is contained in a reservoir within the element and is ~assed under pressure from the reservoir into the capillary. The wash solution thus carries the noninteracted reagent(s) into a waste reservoir located within the element. The label portion o~ the labelled reagent is detected to provide the concentration of the analyte present in the sample. Depending upon the type of label which i5 utilized, it may be detected directly or it may be detected indirectly such as by interaction with yet another reagent which is brought into the caplllary from a reservoir located in the element. Any li~uid in the caplllary which is replaced by the latter reagent will be passed into the waste reservoir.

~l3Cl t)C~C~6 This invention provides a number of significant advantages over the prior art immunoassay elements. Since the interaction of the reagent(s~ and ~he analyte i5 conducted in a very thin, flat capillary tube the fluid str2am i8 very well defined. Therefore, only small volumes of sample and reagents are utilized. Since those volumes are fixed by the volume of thle ca~illary, no volume measurements are required. Whole blood can be utilized as a sample by incorporating appropriate filtration means which separates whole blood into a cellular component and a plasma component in the means for introducing the sample into the element. The sample need not be diluted thereby eliminating prior art dilution steps which require volume measurements. Since the capillary volume ls small, li~uid can be introduced therein without additional means other than capillary action and can be removed therefrom easily by virtue of the small volume by washing. In addition, since the reagents and waste liquid are retained within the ~elf-contained element, the dangers associated with disposing of a potentially in~ectious li~uid are eliminated. The entire element can be disposed of safely such as by burning without contact with the liquids within the element.
BRIEF DESCRIPTION OF THE DRAWINGS
Figure 1 i8 a top view of an element of this invention.
Figure 2 is a cross-sectional side view taken along line 2-2 of ~igure 1.
Figure 3 i5 a cross-sectional view taken along line 3-3 of Figure 1.
Figure 4 is a view of an alternative element o~
this invention.
Figure 5 and 6 are cross-sectional views of the element of this invention illustrating its mode of operation.

~l3~ [30Ç;

Figure 7 is a top ~iew of an alternative element of this invention; and Flgure 8 is a cross-sectional side view taken along llne 8-~ of Flgure 7.

DESCRIPTION OF SPECIFIC EMBODIMEN'rS
Referrlng to Figures 1-3, the analytical element of this invention 10 ls fQrmed so that a top ~ection and a bottom section are ~oined together to form a hou~ing which inoludes a capillary tube, a sample introdu~ing means, at least one reservoir for a reagent and/or wash solution, and a waste reservoir wherein the capillary, each reservoir and the sample introducing means are in fluid communication with each other. The element 10 typically has a width of from about 10 to about 20 mm, preferably about 15 mm, an overall length of from about 30 mm to about 50 mm, preferably about 40 mm, and a height of ~rom about 3 to about 8 mm, preferably about 5 mm. The bottom section can comprise a plate 12 which is adhered to the top section comprising a plurality of walls and reservoirs as shown generally in Figures 1 and 2. A capillary tube 14 is provided in a central portion of the element 10 and the capillary tube 14 includes at least one surface, here shown as the bottom surface 16 of the tube, which has bonded to at least a portion thereof a first reagent which i5 capable of interacting, either directly or indlrectly, with the a~alyte of interest present in the sample. Thus, the surface of the capillary tube which has the first reagent bonded thereto defines the reaction zone of the element. The capillary tube 14 is typically from about 0.02 mm to about 0.5 mm, and preferably from about 0.05 mm to about 0.2 mm, in height; from about 1 to about 10 mm, and pre~erably from about 3 to about 5 mm, in width; and from about 5 to about 25 mm, and preferably from about 8 to about 12 mm, in length. The surfaces of the capillary tube 14 may be smooth or roughened, e.g., grooved. In the ~8 --13~

latter instances a larger amount of the first reagent may be bonded to a given unit area of the surface.
An opening 18 is provided in the housing to permit the sample to be introduced into the element 10.
The sample volumes typically re~uired for use with the immunoassay element range ~rom about 20 to about 100 ul, preferably from about 30 to about 50 ul. As shown in Figures 1-3, the sample introduction means includes an optional filter 20 and a membrane layer 22. The filter may be provided in embodiments where it i8 de~ired to use whole blood samples for example, to remove components therefrom. The filter element 20 may be any suitable material, synthetic or naturally occurring, or a mixture of each type, whlch is capable of removing from the fluid sample any component(s) which could interfere with the analysis and which i8 inert to the analyte(s) of interest, that is, will not prevent any significant amount of said analyte(s) from passing through the filter whether because of adsorption, reac~ion or otherwise. The type of filter material utili~ed in any instance is dependent upon the type of biological ~luid to be analyzed. For, example, a microporous filter element may be used to remove bacteria cells or microorganisms from the sample fluid. In a preferred embodiment where the sample is whole blood, the filter element comprises a fibrous material which is capable of separating cells, e.g., erythrocytes, leucocytes, etc., from plasma or serum. Typical suitable fibrous materials include glass, guartz, cellulose acetate, cellulose, synthetic polymeric fibers uch as polyamides or polyesters, and the like. The fibrous material may be treated with a material such a~ gelatin, either inert or deionized, or serum albumin to substantially reduce or eliminate any binding thereto by an analyte of interest. Further, ~he filter element 20 may be impregnated with a material which is capable of removing specific components from the fluid sample, for _g _ ~3~ 6 example, lipoproteins. Titanium dioxide is suitable for this purpose. The membrane 22 may contain a labelled reagent which is interactive with either the analyte of interest or the ~irst reagent which is fixed to the S surface of the capillary.
A first reservolr 24 is provided which contains a wash solution. The wash li~uid may be simply sealed in the reservoir or it may be provided in a pod 26 as illustrated. A second reservoir 28 may also be ~rovided, when necessary, for a reagent which is capable of interacting with the label moiety of the labelled reagent in order to render the label detectable as where the label is an enzyme and the reagent is a substrate for the enzyme. As illustrated the reagent is provided in a pod 30. In one embodiment, the reagent which is adapted to render the label detectable can be provided in a separate reservoir 32 in a paper matrix, membrane matrix or the like. Pod 26, as shown, is enclosed within reservoir 24 by means of a thin layer 34 of a flexible material such as aluminum foil or the like which seals the reservoir 2~
from the atmosphere. By exerting pressure on the thin layer 34 such as with a plunger (not shown) and consequently applying pressure on pod 26, the pod can be burst and the liquid therein caused to enter the capillary tube 14 by passing through opening 38, passageway 40 and opening 42. In like manner, the liauid in pod 30, when present, can be caused to enter the capillary tube 14. A
transparent zone, or window, 44 is provided in the housing above at least the reaction zone of the c~pillary tube in order to permit readout of the reaction zone by a conventional detection device such as a densitometer, fluorimeter or the like.
A waste reservoir 46 is provided to receive waste sample, wash reagent or waste wash solution ~rom the capillary tube. The waste reservolr 46 may include a liauid absorbent 48 and open areas 50 and 52 in order to ~3~

provide sufficient volume capacity to retain all the waste sample, reagent, etc., utilized in the assay. Also, there may be provided an air hole in the top surface 65 of waste reservoir 46 to allow the waste liquids to fill the reservoir. In the embodiment illustrated in Figures 1-3 the waste liquids pass from the capillary tube 14 through membrane 22 and into waste reservoir 46.
The immunoassay elements of the invention may be fabricated by ~nitially making two separate parts, i.e., an upper part and a lower part. The bottom part would include the b~ttom surface o~ the capillary tube and the wall and bottom sur~ace o~ the reservoirs. After binding the f.irst reagent to the capillary tube surface and depositing the wash solution and, where neçessary the additional reagent, in the appropriate reservolrs, the upper and lower parts are brought together and sealed such as by ultrasonic welding, heat and pressure, etc. The housing of the element may be made ~rom any suitable material including, for example, synthetic polymeric materials which are easily moldable. A ~referred material is polystyrene which has been treated with gamma radiation to enhance its ability to bind antigens or antibodies.
The housing may be transparent or opaque, provided that where it is opague there is a transparent window to allow detection of the labelled spscies which is obtained as a result of the interaction between the analyte and reagents.
Figure 4, wherein the referenced elements having the same numerical reference as those shown in Figures 1 through 3 are the same elements, illustrates another e~bodiment of the invention. In this embodiment the capillary tube 14 is modified to include a second caplllary outlet 56, which permits air passage therethrough, and there is also provided a hole 58 in the upper surface of the waste reservoir in the vicinity of outlet 56 to assure that the ca~illary tube 14 becomes filled with liauid. The element may also include an air ~o~

: hole 62 in the upper surface of the waste reservoir as ~ shown in Figures 1-3. The air hole~ 58 and 62 may be made - suitably small, or they may be covered with a hydro~hobic material such as a hydro~hobic ~orou~ membrane, to allow passage of air while preventing passage of liquid.
~ igures 5 and 6 illustrate an embodiment of the immunoassay element wherein there is ~rovided one reagent reservoir containing a volume of reagent sufficient to function both as a wash liauid and as a reagent for .interacting with the reagent bound to the capillary and/or the analyte to render the label detectable. In operation a drop of whole blood 64 is introduced through opening 18 onto filter 20 which separates the blood cells from the blood plasma. The plasma passes into membrane 22 which includes a labelled reagent which interacts with at least part the sam~le analyte to form a con~ugate. The plasma, together with the con~ugate migrates into capillary tube 14 by means of capillary action. After the caplllary has been filled and the plasma is allowed to incubate within the capillary for the re~uired period of time during which the interactions between the sample analyte, labelled rsagent and the reagent bound to the surface of the capillary tube go to completion, plunger 66 is pushed downwardly 50 as to contact foil layer 34 and exert sufficient pressure on pod 26 to cause the pod to be ruptured. The reagent is carried into the capillary tube 14 and at the same time it displaces the plasma containing any unbound analyte, labelled reagent or con~ugate suspended or dissolved therein. The displaced plasma passes into waste reservoir 46 containing absorbent material 48. During the preceding incubation period, substantially all of the analyte will have interacted with the labelled reagent and the reagent bound to the surface of the capillary tube. By virtue of these interactions the bound species which is formed will remain in the capillary and i8 not displaced by the incoming rea~ent.

~3~ 6 After a suitable incubation ~eriod to allow the reagent to render the label detectable, it is detected through trans~arent w~ndow 44 by means of an appropriate conventional detection apparatus.
Figures ~ and 8 illustrate an immunoassay element according to the invention where the wash liquid and/or reagent reservoirs are filled with the liquid(s~ and pods are not utilized. As pointed out previously such reservoirs can be formed in two parts which are then : lO brought together, capillary tube 14 by means of capillary action. The element includes a filling capillary 70 which allows the ~luid which passes through membrane Z2 to enter capillary tube 14 by capillary action. In this embodiment, fluid which is initially present in capillary tube 14 and then expelled therefrom by wash solution and/or a reagent passes into waste reservoir without having to ~ass through membrane 22. The other elements of the device and the operation thereof are as described previously.
The immunoassay element of the invention may be used to carry out any of the known immunometric, sandwich or competitive assays for an analyte, e.g., an antigen or an antibody. These assays are well known to those skilled in the art and extensive discussion thereof is not re~uired here. Briefly, in one type of sandwich assaywherein the assay element includes two reservoirs for reagent and/or wash fluid, a sample is applied to the sample inlet, migrates through a layer containing a labelled reagent thereby imbibing labelled reagent and passes into the capillary by virtue of capillary action.
During a suitable incubation period the analyte in the sample interacts with the labelled reagent and with the . reagent bound to a surface of the capillary.
Subse~uently, wash liauid is introduced from a reservoir within the element and passes into the capillary to displace the fluid present therein to~ether with any ~30~ 6 uninteracted labelled reagent. The d~splaced fluid is forced into the waste reservoir. Another reagent, which is adapted to render the label moiety of the labelled reagent detectable, i9 introduced into the capillary and displaces the wash liquid which 15 forced into the waste reservoir. After a sultable incubation period the detectable signal formed ln the capillary tube is measured.
In a second embodiment wherein a sandwich assay i5 conducted, three reservoirs containing wash solution and/or reagent are employed in the element. In the first step, a sample containin~ the analyte is introduced into the element and ~asses by ca~illary action into the capillary wherein the analyte interacts with the fixed first reagent in the capillary. In a second step, a labelled reagent which interacts with the analyte from a reservoir of the element i5 introduced into the capillary. In a third step, a wash solution from a reservoir of the element is introduced into the capillary to replace the liquid therein and cause the uninteracted liquid to pass into the waste reservoir. In a fourth ste~, a liquid containing a substrate which interacts with the label of the labelled reagent to render the label detectable is passed from a third reservoir into the capillary to replace the wash li~uid therein and to cause the substrate to interact with the label fixed to the first reagent. The label rendered detectable then is observed through a transparent opening in the capillary.
As an example of the competitive as~ay, the sam~le containing an analyte is introduced lnto the element and mixed with a labelled analyte located within a porous layer within the sample introduction means. The sample, analyte and the labelled analyte then are introduced into the capillary by means of capillary action wherein they are interacted with a fixed first rea~ent which interacts with the analyte or labelled analyte.
Thereafter, the noninteracted s~ecies is removed from the ~14-~L3~

ca~illary by means of sufficient reagent which interactswith the label to form a detectable molety. In this embodiment, only one reagent reservoir i5 required. In an alternative embodiment, the capillary is first washed with a wa~sh liauid from one reservoir and then with a reagent which interacts with the label to form a detectable moiety in a second step. In this latter embodiment, two reservoirs are required.
Any chemical interaction which effects a change in the radiation emission in either the label of the labelled reagent or a reagent which interacts with the label to cause a change in radiation emission to provide a detectable signal can be exploited in accordance with the element of the invention. For example, any change in fluorescence, chemiluminescence, color or other change in visible or near visible radiation can be exploited. Thus, the label utilized in the labelled reagent may be directly or indirectly detectable. The label may be a ~luorophore, chromophore, chemiluminophore, a phosphor or an enzyme.
Where the label is an enzyme it can be one which interacts with a substrate to cause a change in absorption where the substrate is chromogen, in fluorescence if the substrate is a fluorophore, in chemiluminescence if the substrate is a chemiluminescent precursor or in phosphorescence where the subs~rate is a ~hosphor.
Although the invention has been described with respect to various embodiments thereof, it is not intended to be limited thereto but rather those skilled in the art will recognize that variations and modifications may be made therein which are within the spirit of the invention and the scope of the appended claims.

Claims (24)

THE EMBODIMENTS OF THE INVENTION IN WHICH AN EXCLUSIVE
PROPERTY OR PRIVILEGE IS CLAIMED ARE DEFINED AS FOLLOWS:
1. A self-contained element for analysis of a liquid sample containing a component to be determined which comprises:
a housing containing a capillary tube having opposed interior, top and bottom surfaces, a portion of at least one of said top and bottom surfaces having bound thereto a first reagent consisting of either said component to be determined or the binding partner of said component to be determined, said bound first reagent defining a reaction zone and interactive with said component to be determined to form a first nondiffusible species, and at least one of said top and bottom surfaces including a transparent area to permit measurement of radiation from at least a portion of said reaction zone;
a labelled second reagent, said second reagent consisting of said component to be determined or the binding partner of said component to be determined and being interactive with said first nondiffusible species or said first reagent to form a second labelled species;
means for introducing at least a portion of said sample and said labelled second reagent into said capillary tube by capillary forces;
at least one reactant reservoir containing a wash solution or a third reagent which is interactive with said second labelled species, said at least one reactant reservoir being in fluid communication with said capillary tube;

a waste reservoir in fluid communication with said capillary tube adapted to receive waste fluid from said capillary tube;
and said means for introducing said sample and said labelled second reagent, said at least one reactant reservoir and said waste reservoir being housed within said housing.

-16a-
2. The element of claim 1 wherein said means for introducing said sample into said capillary comprises an inlet and a porous membrane in fluid communication with said capillary.
3. The element of claim 2 including a filter element contiguous with said membrane.
4. The element of claim 1 wherein said means for introducing said sample includes said laballed reagent adapted to be introduced into said capillary with said sample.
5. The element of any one of claims 2 or 3 wherein said porous membrane contains said labelled reagent adapted to be introduced into said capillary with said sample.
6. The element of any one of claims 1, 2, 3 or 4 having one reactant reservoir.
7. The element of any one of claims 1, 2, 3 or 4 having two reactant reservoirs.
a. The element of any one of claims 1, 2, 3, or 4 having three reactant reservoirs.
9. The element of any one of claims 1, 2, 3 or 4 wherein said waste reservoir contains a liquid absorbing material.
10. The element of claim 4 wherein said labelled reagent is a labelled antibody.
11. The element of claim 4 wherein the label of said labelled reagent is an enzyme.
12. The element of any one of claims 2 or 3 wherein said porous membrane contains a labelled antibody.
13. The element of any one of claims 2 or 3 wherein the label of said labelled reagent is an enzyme.
14. A process for analyzing for an analyte in a liquid sample within the confines of a self-contained element housing which comprises:

introducing a liquid sample into a porous layer within said element, said porous layer containing a labelled reagent which is interactive with said analyte;
passing said analyte and said labelled reagent by capillary force into a capillary having opposed top and bottom surfaces and a first reagent bound to at least one of said top and bottom surfaces, said first reagent being interactive with said analyte and said labelled reagent to form a first nondiffusible species comprising said labelled reagent, said analyte and said first reagent;
washing the interior of said capillary with a liquid composition positioned in a reservoir within said element and in fluid communication with said capillary to pass the portion of the sample noninteracted with said first reagent into a waste reservoir located within said element, and rendering said labelled reagent detectable.
15. The process of claim 14 wherein said labelled reagent is rendered detectable after said analyte interacts with said first reagent and said labelled reagent.
16. The process of claim 14 wherein said labelled reagent is rendered detectable by washing said capillary with a sufficient amount of a liquid composition to remove noninteracted sample from said capillary and to form a deteatable species with substantially all of the labelled reagent interacted with said analyte in said capillary.
17. The process of claim 14 wherein said labelled reagent is rendered detectable by washing said capillary with a wash solution to remove noninteracted sample from said capillary and replacing said wash solution in said capillary with a second liquid reagent.
18. The process of any one of claims 14, 15, 16 or 17 wherein said labelled reagent is labelled with an enzyme.

18a
19. A process for analyzing for an analyte in a liquid sample within the confined of a self-contained element housing which comprises:
introducing by capillary forces a liquid sample into a capillary having opposed top and bottom surfaces and a first reagent bound to at least one of said surfaces, said first reagent being interactive with said analyte to form a first nondiffusible species comprising said analyte and said first reagent;
washing said capillary with a liquid wash composition positioned in a reservoir within said element, said reservoir being in fluid communication with said capillary to pass the portion of the sample noninteracted with said first reagent into a waste reservoir located within said element, passing a labelled reagent interactive with said analyte from a reservoir within said element into said capillary and directing the liquid wash composition from said capillary into said waste reservoir;
incubating said labelled reagent and said first nondiffusible species to effect interaction between said analyte and said first reagent to form a second nondiffusible species;
and rendering said second nondiffusible species detectable.
20. The process of claim 19 wherein said labelled reagent is rendered detectable by washing said capillary with a sufficient amount of a liquid composition to remove noninteracted samples from said capillary and to form a detectable species with substantially all of the labelled reagent interacted with said analyte in said capillary.
21. A process for analyzing for an analyte in a liquid sample within the confines of a self-contained element housing which comprises:

introducing a liquid sample containing an analyte into a porous layer within said element, said porous layer containing a labelled reagent:
introducing by capillary forces the labelled reagent and said analyte into a capillary having an inner surface and a first reagent bound to said inner surface, said first reagent being interactive with said analyte to form a first nondiffusible species comprising said analyte and said first reagent and a second nondiffusible species comprising said first reagent and said labelled reagent;
washing said capillary with a liquid composition positioned in a reservoir within said element and in fluid communication with said capillary to pass the portion of the sample and labelled reagent noninteracted with said first reagent into a waste reservoir located with in said element and rendering said labelled reagent detectable.
22. The process of claim 21 wherein said labelled reagent is rendered detectable after said labelled reagent interacts with said first reagent.
23. The process of claim 21 wherein said labelled reagent is rendered detectable by washing said capillary with a sufficient amount of liquid composition to remove noninteracted sample from said capillary and to form a detectable species with substantially all of the labelled reagent interacted with said first reagent in said capillary.
24. The process of claim 21 wherein said labelled reagent is rendered detectable by washing said capillary with a wash solution to remove noninteracted sample from said capillary and replacing said wash solution in said capillary with a second liquid reagent.
CA000560295A 1987-03-03 1988-03-02 Self-contained immunoassay element Expired - Fee Related CA1300006C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US021,136 1979-03-16
US07/021,136 US4918025A (en) 1987-03-03 1987-03-03 Self contained immunoassay element

Publications (1)

Publication Number Publication Date
CA1300006C true CA1300006C (en) 1992-05-05

Family

ID=21802544

Family Applications (1)

Application Number Title Priority Date Filing Date
CA000560295A Expired - Fee Related CA1300006C (en) 1987-03-03 1988-03-02 Self-contained immunoassay element

Country Status (14)

Country Link
US (1) US4918025A (en)
EP (1) EP0281201B1 (en)
JP (1) JPH0616047B2 (en)
AT (1) ATE88814T1 (en)
AU (1) AU605257B2 (en)
CA (1) CA1300006C (en)
DE (1) DE3880531T2 (en)
ES (1) ES2041775T3 (en)
FI (1) FI96639C (en)
GR (1) GR3008296T3 (en)
NO (1) NO173410C (en)
NZ (1) NZ223637A (en)
WO (1) WO1988006731A1 (en)
ZA (1) ZA88550B (en)

Families Citing this family (110)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5622871A (en) 1987-04-27 1997-04-22 Unilever Patent Holdings B.V. Capillary immunoassay and device therefor comprising mobilizable particulate labelled reagents
US5137808A (en) * 1987-04-07 1992-08-11 Syntex (U.S.A.) Inc. Immunoassay device
DE3856421T2 (en) 1987-04-27 2000-12-14 Unilever Nv Specific binding test procedures
US5006474A (en) * 1987-12-16 1991-04-09 Disease Detection International Inc. Bi-directional lateral chromatographic test device
EP0321736B1 (en) * 1987-12-23 1994-06-01 Abbott Laboratories Agglutination reaction device
WO1990005906A1 (en) * 1988-11-14 1990-05-31 Idexx Corporation Dual absorbent analyte detection
US6645758B1 (en) 1989-02-03 2003-11-11 Johnson & Johnson Clinical Diagnostics, Inc. Containment cuvette for PCR and method of use
CA1338505C (en) * 1989-02-03 1996-08-06 John Bruce Findlay Containment cuvette for pcr and method of use
US5229297A (en) * 1989-02-03 1993-07-20 Eastman Kodak Company Containment cuvette for PCR and method of use
US6352862B1 (en) 1989-02-17 2002-03-05 Unilever Patent Holdings B.V. Analytical test device for imuno assays and methods of using same
US5147609A (en) * 1989-05-19 1992-09-15 Pb Diagnostic Systems, Inc. Assay element
AU642444B2 (en) * 1989-11-30 1993-10-21 Mochida Pharmaceutical Co., Ltd. Reaction vessel
AU635008B2 (en) * 1989-12-13 1993-03-11 Genelabs Diagnostics Pte Ltd Analytical apparatus and method for automated blot assay
US5098660A (en) * 1990-01-08 1992-03-24 Eastman Kodak Company Transfer apparatus for chemical reaction pack
US5922615A (en) 1990-03-12 1999-07-13 Biosite Diagnostics Incorporated Assay devices comprising a porous capture membrane in fluid-withdrawing contact with a nonabsorbent capillary network
WO1991018291A1 (en) * 1990-05-18 1991-11-28 Pb Diagnostic Systems, Inc. Solid support having antibodies immobilized thereon
US5147606A (en) * 1990-08-06 1992-09-15 Miles Inc. Self-metering fluid analysis device
US5208163A (en) * 1990-08-06 1993-05-04 Miles Inc. Self-metering fluid analysis device
EP0471940B1 (en) * 1990-08-16 1994-12-07 Drägerwerk Aktiengesellschaft Colorimetric detecting device with a reagent storage reservoir
US5998220A (en) 1991-05-29 1999-12-07 Beckman Coulter, Inc. Opposable-element assay devices, kits, and methods employing them
US5869345A (en) * 1991-05-29 1999-02-09 Smithkline Diagnostics, Inc. Opposable-element assay device employing conductive barrier
US6168956B1 (en) 1991-05-29 2001-01-02 Beckman Coulter, Inc. Multiple component chromatographic assay device
US5877028A (en) 1991-05-29 1999-03-02 Smithkline Diagnostics, Inc. Immunochromatographic assay device
US5468648A (en) * 1991-05-29 1995-11-21 Smithkline Diagnostics, Inc. Interrupted-flow assay device
US5607863A (en) * 1991-05-29 1997-03-04 Smithkline Diagnostics, Inc. Barrier-controlled assay device
US6007999A (en) * 1991-07-31 1999-12-28 Idexx Laboratories, Inc. Reversible flow chromatographic binding assay
US5726010A (en) * 1991-07-31 1998-03-10 Idexx Laboratories, Inc. Reversible flow chromatographic binding assay
US5726026A (en) * 1992-05-01 1998-03-10 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5427739A (en) * 1992-08-27 1995-06-27 Schering-Plough Healthcare Products, Inc. Apparatus for performing immunoassays
US5637467A (en) * 1992-10-13 1997-06-10 Behringwerke Ag Heterogeneous assay using a pendulous drop
US5500187A (en) * 1992-12-08 1996-03-19 Westinghouse Electric Corporation Disposable optical agglutination assay device and method for use
US5798215A (en) * 1993-02-18 1998-08-25 Biocircuits Corporation Device for use in analyte detection assays
US5503985A (en) * 1993-02-18 1996-04-02 Cathey; Cheryl A. Disposable device for diagnostic assays
US5372948A (en) * 1993-03-17 1994-12-13 Miles Inc. Assay and reaction vessel with a compartmentalized solubilization chamber
GB9307319D0 (en) * 1993-04-07 1993-06-02 British Tech Group Liquid transfer devices
US5840573A (en) * 1994-02-01 1998-11-24 Fields; Robert E. Molecular analyzer and method of use
AU6788796A (en) * 1995-08-03 1997-03-05 Akzo Nobel N.V. Diagnostic device
EP0781997A1 (en) * 1995-12-27 1997-07-02 FUJIREBIO Inc. Instrument for simplified immunoassay and simplified immunoassay method using the instrument
US5945345A (en) * 1996-08-27 1999-08-31 Metrika, Inc. Device for preventing assay interference using silver or lead to remove the interferant
US5879951A (en) 1997-01-29 1999-03-09 Smithkline Diagnostics, Inc. Opposable-element assay device employing unidirectional flow
US5939252A (en) 1997-05-09 1999-08-17 Lennon; Donald J. Detachable-element assay device
DE69830416T2 (en) * 1997-10-07 2005-11-10 Ucb S.A. TEST APPARATUS AND METHOD FOR DETERMINING ANALYTES IN A LIQUID MILK PRODUCT
US6120733A (en) * 1997-11-12 2000-09-19 Goodman; David B. P. Self-contained assay device
US5817522A (en) * 1997-11-12 1998-10-06 Goodman; David B. P. Self-contained assay device and method
EP1179585B1 (en) 1997-12-24 2008-07-09 Cepheid Device and method for lysis
US6267722B1 (en) 1998-02-03 2001-07-31 Adeza Biomedical Corporation Point of care diagnostic systems
US6394952B1 (en) 1998-02-03 2002-05-28 Adeza Biomedical Corporation Point of care diagnostic systems
US6184040B1 (en) 1998-02-12 2001-02-06 Polaroid Corporation Diagnostic assay system and method
US6663833B1 (en) 1998-03-10 2003-12-16 Strategic Diagnostics Inc. Integrated assay device and methods of production and use
USD434153S (en) * 1998-04-20 2000-11-21 Adeza Biomedical Corporation Point of care analyte detector system
USD432244S (en) * 1998-04-20 2000-10-17 Adeza Biomedical Corporation Device for encasing an assay test strip
US6228657B1 (en) * 1998-09-29 2001-05-08 The United States Of America As Represented By The Secretary Of The Army Environmental material ticket reader and airborne hazard detection system
US6555060B1 (en) 1998-10-14 2003-04-29 Polaroid Corporation Apparatus for performing diagnostic testing
US6495373B1 (en) 1998-10-14 2002-12-17 Polaroid Corporation Method and apparatus for performing diagnostic tests
US6331715B1 (en) 1998-10-14 2001-12-18 Polaroid Corporation Diagnostic assay system and method having a luminescent readout signal
US6431476B1 (en) 1999-12-21 2002-08-13 Cepheid Apparatus and method for rapid ultrasonic disruption of cells or viruses
US7914994B2 (en) 1998-12-24 2011-03-29 Cepheid Method for separating an analyte from a sample
US6328930B1 (en) 1999-02-11 2001-12-11 Polaroid Corporation Apparatus for performing diagnostic testing
US9073053B2 (en) 1999-05-28 2015-07-07 Cepheid Apparatus and method for cell disruption
AU782343B2 (en) 1999-05-28 2005-07-21 Cepheid Apparatus and method for analyzing a fluid sample
US20040200909A1 (en) * 1999-05-28 2004-10-14 Cepheid Apparatus and method for cell disruption
US8815521B2 (en) 2000-05-30 2014-08-26 Cepheid Apparatus and method for cell disruption
US6818185B1 (en) * 1999-05-28 2004-11-16 Cepheid Cartridge for conducting a chemical reaction
US6664104B2 (en) 1999-06-25 2003-12-16 Cepheid Device incorporating a microfluidic chip for separating analyte from a sample
WO2002035216A1 (en) * 2000-10-25 2002-05-02 Miller Michael M Multilayer analytical element and method for determination of analytes in fluids containing interfering substances
US6641782B1 (en) 2000-11-15 2003-11-04 Polaroid Corporation Apparatus for performing diagnostic testing
US7435384B2 (en) * 2001-01-08 2008-10-14 Leonard Fish Diagnostic instrument with movable electrode mounting member and methods for detecting analytes
NO314206B1 (en) * 2001-04-30 2003-02-10 Erling Sundrehagen Quantitative chemical analysis method, device / device, and application of said method and analysis set
WO2003005020A1 (en) * 2001-06-15 2003-01-16 J.Mitra & Co. Ltd. See through testing device
CN1327223C (en) * 2001-10-29 2007-07-18 爱科来株式会社 Test apparatus
JP2003166910A (en) * 2001-11-30 2003-06-13 Asahi Kasei Corp Liquid-feeding mechanism and analyzer provided with the same
US8211657B2 (en) * 2002-04-29 2012-07-03 The Board Of Trustees Of The University Of Arkansas Capillary-column-based bioseparator/bioreactor with an optical/electrochemical detector for detection of microbial pathogens
AU2003259751C1 (en) 2002-08-13 2009-09-17 Qiagen Sciences, Llc Devices and methods for detecting amniotic fluid in vaginal secretions
JP3865134B2 (en) * 2003-01-09 2007-01-10 横河電機株式会社 Biochip cartridge
ES2439225T3 (en) 2004-01-26 2014-01-22 President And Fellows Of Harvard College System and method for fluid supply
US8030057B2 (en) 2004-01-26 2011-10-04 President And Fellows Of Harvard College Fluid delivery system and method
US20060078471A1 (en) * 2004-10-12 2006-04-13 Witty Thomas R Apparatus and method for a precision flow assay
US7507374B2 (en) * 2005-06-28 2009-03-24 American Bio Medica Corp. Saliva sample testing device
US7816122B2 (en) * 2005-10-18 2010-10-19 Idexx Laboratories, Inc. Lateral flow device with onboard reagents
WO2008097268A2 (en) * 2006-07-26 2008-08-14 Detectachem, Llc. Apparatus and method for detection of trace chemicals
JP4910722B2 (en) * 2007-01-26 2012-04-04 日本電気株式会社 Gas detector
NL1033365C2 (en) 2007-02-09 2008-08-12 Medavinci Dev B V Device and method for separating and analyzing blood.
EP2089159B1 (en) * 2007-05-03 2013-01-23 Clondiag GmbH Assays
WO2008137008A2 (en) 2007-05-04 2008-11-13 Claros Diagnostics, Inc. Fluidic connectors and microfluidic systems
CN101970111B (en) 2007-06-21 2013-09-11 简·探针公司 Instrument and receptacles for performing processes
US9795697B2 (en) 2007-09-19 2017-10-24 Opko Diagnostics, Llc Liquid containment for integrated assays
JP2009198259A (en) * 2008-02-20 2009-09-03 Research Institute Of Biomolecule Metrology Co Ltd Chemical substance recovering kit, biomolecule inspection system, and biomolecule inspection method
NL2001577C2 (en) * 2008-05-14 2009-11-17 Medavinci Dev B V Device and method for separating and analyzing blood.
US10203310B2 (en) 2009-01-26 2019-02-12 Detectachem Llc Chemical detection of substances by utilizing a sample medium impregnated with solid test chemicals
WO2010087999A1 (en) 2009-02-02 2010-08-05 Claros Diagnostics, Inc. Structures for controlling light interaction with microfluidic devices
JP5430995B2 (en) * 2009-03-30 2014-03-05 富士フイルム株式会社 Assay method and assay device
EP2823427B1 (en) 2012-03-05 2020-12-16 OY Arctic Partners AB Computer systems, methods and computer readable storage medium for predicting risk of prostate gland volume
TWI484182B (en) * 2012-11-07 2015-05-11 Taidoc Technology Corp Reaction vessel, assay device, and measuring method
US9724689B2 (en) * 2012-11-20 2017-08-08 Detectachem Llc Colorimetric test system designed to control flow of simultaneously released chemicals to a target area
CN105008925B (en) 2013-01-02 2017-09-26 N-Dia有限责任公司 The method for predicting the pregnant woman childbirth time
CA2900708C (en) 2013-03-13 2021-06-15 Opko Diagnostics, Llc Mixing of fluids in fluidic systems
CN105849560A (en) * 2013-11-21 2016-08-10 雅托莫诊断私人有限公司 Fluid control in integration test device
EP3151965B1 (en) 2014-06-04 2021-02-24 Edan Instruments, Inc. Sample collection and analysis devices
JP6588910B2 (en) 2014-06-30 2019-10-09 Phcホールディングス株式会社 Sample analysis substrate, sample analysis apparatus, sample analysis system, and program for sample analysis system
US10309976B2 (en) 2014-06-30 2019-06-04 Phc Holdings Corporation Substrate for sample analysis, sample analysis device, sample analysis system, and program for sample analysis system
US10539582B2 (en) 2014-06-30 2020-01-21 Phc Holdings Corporation Substrate for sample analysis, sample analysis device, sample analysis system, and method for removing liquid from liquid that contains magnetic particles
EP3163306A4 (en) 2014-06-30 2018-01-24 Panasonic Healthcare Holdings Co., Ltd. Substrate for sample analysis, and sample analysis apparatus
CN107209193B (en) 2014-12-12 2019-08-02 普和希控股公司 Sample analysis substrate, sample analyzer, sample analysis system and sample analysis system program
US10279345B2 (en) 2014-12-12 2019-05-07 Opko Diagnostics, Llc Fluidic systems comprising an incubation channel, including fluidic systems formed by molding
GB2535998A (en) * 2015-02-27 2016-09-07 Intelligent Fingerprinting Ltd A device for receiving and analysing a sample
US10408825B2 (en) * 2015-05-19 2019-09-10 Electronics And Telecommunications Research Institute Biosensor
US10656164B2 (en) 2016-12-22 2020-05-19 Qiagen Sciences, Llc Screening asymptomatic pregnant woman for preterm birth
US10935555B2 (en) 2016-12-22 2021-03-02 Qiagen Sciences, Llc Determining candidate for induction of labor
EP3748360A4 (en) * 2018-02-02 2021-11-03 Nippon Chemiphar Co., Ltd. Biochemical reaction substrate and analysis device
WO2023084050A1 (en) * 2021-11-11 2023-05-19 Radiometer Medical Aps Apparatus comprising cartridge with absorbing element

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3799742A (en) * 1971-12-20 1974-03-26 C Coleman Miniaturized integrated analytical test container
SE388694B (en) * 1975-01-27 1976-10-11 Kabi Ab WAY TO PROVIDE AN ANTIGEN EXV IN SAMPLES OF BODY WHEATS, USING POROST BERAR MATERIAL BONDED OR ADSORBING ANTIBODIES
US4116638A (en) * 1977-03-03 1978-09-26 Warner-Lambert Company Immunoassay device
FR2389134A1 (en) * 1977-04-29 1978-11-24 Seroa Analytical use of capillary micro-tubes - as measuring and reaction vessel esp. for biochemical analysis
US4472353A (en) * 1980-08-07 1984-09-18 Gerald Moore Gas detector badge
NZ199286A (en) * 1980-12-22 1986-05-09 Commw Serum Lab Commission A method of detecting antibodies or an antigenic or haptenic substance in a sample
US4517288A (en) * 1981-01-23 1985-05-14 American Hospital Supply Corp. Solid phase system for ligand assay
US4426451A (en) * 1981-01-28 1984-01-17 Eastman Kodak Company Multi-zoned reaction vessel having pressure-actuatable control means between zones
DE3134611A1 (en) * 1981-09-01 1983-03-10 Boehringer Mannheim Gmbh, 6800 Mannheim METHOD FOR CARRYING OUT ANALYTICAL PROVISIONS AND MEANS SUITABLE FOR THIS
US4447546A (en) * 1982-08-23 1984-05-08 Myron J. Block Fluorescent immunoassay employing optical fiber in capillary tube
US4673657A (en) * 1983-08-26 1987-06-16 The Regents Of The University Of California Multiple assay card and system
WO1985002466A1 (en) * 1983-12-02 1985-06-06 Vertrik Bioteknik Ab A device for chemical analyses and use thereof
DE3484505D1 (en) * 1983-12-19 1991-05-29 Daiichi Pure Chemicals Co Ltd IMMUNTEST.
DE3425008A1 (en) * 1984-07-06 1986-02-06 Boehringer Mannheim Gmbh, 6800 Mannheim METHOD AND DEVICE FOR CARRYING OUT ANALYTICAL PROVISIONS
IL73938A (en) * 1984-01-02 1989-09-28 Boehringer Mannheim Gmbh Process and reagent for the determination of polyvalent antigen by incubation with three different receptors
US4708931A (en) * 1984-06-01 1987-11-24 Regents Of University Of California Laminated rod having alternating detection and spacer layers for binding assays
SE458643B (en) * 1984-12-07 1989-04-17 Pharmacia Ab DEVICE WITH COLUMN ELEMENTS DIVIDED IN SAMPLE PARTY AND REFERENCE PATRIAR
DE3585780D1 (en) * 1984-12-24 1992-05-07 Abbott Lab ANALYTICAL DEVICE AND METHOD FOR USE THEREOF.
EP0221105A1 (en) * 1985-04-29 1987-05-13 Hichem Diagnostics, Inc., Dba Bural Technologies Diagnostic test kit
US4740475A (en) * 1986-03-28 1988-04-26 Medi-Scan, Inc. Integral substance detection device and method
US4806316A (en) * 1987-03-17 1989-02-21 Becton, Dickinson And Company Disposable device for use in chemical, immunochemical and microorganism analysis
US4849340A (en) * 1987-04-03 1989-07-18 Cardiovascular Diagnostics, Inc. Reaction system element and method for performing prothrombin time assay

Also Published As

Publication number Publication date
US4918025A (en) 1990-04-17
ES2041775T3 (en) 1993-12-01
AU605257B2 (en) 1991-01-10
EP0281201A1 (en) 1988-09-07
WO1988006731A1 (en) 1988-09-07
NZ223637A (en) 1990-05-28
FI883599A0 (en) 1988-08-01
AU1294988A (en) 1988-09-26
FI96639C (en) 1996-07-25
NO884658L (en) 1988-10-19
JPH01502526A (en) 1989-08-31
DE3880531T2 (en) 1993-09-09
FI883599A (en) 1988-09-04
NO173410C (en) 1993-12-08
ZA88550B (en) 1988-08-30
DE3880531D1 (en) 1993-06-03
NO884658D0 (en) 1988-10-19
ATE88814T1 (en) 1993-05-15
JPH0616047B2 (en) 1994-03-02
GR3008296T3 (en) 1993-09-30
EP0281201B1 (en) 1993-04-28
NO173410B (en) 1993-08-30
FI96639B (en) 1996-04-15

Similar Documents

Publication Publication Date Title
CA1300006C (en) Self-contained immunoassay element
CA1189431A (en) Multi-zoned reaction vessel having pressure- actuatable control means between zones
US6120733A (en) Self-contained assay device
AU764945B2 (en) Multiple analyte assay device with sample integrity monitoring system
US5399486A (en) Disposable unit in diagnostic assays
US6248598B1 (en) Immunoassay that provides for both collection of saliva and assay of saliva for one or more analytes with visual readout
EP0034049B1 (en) Test device for analysis of a plurality of analytes
US5288648A (en) Multiwell stat test
US7347972B1 (en) Multiple analyte assay device
US8202487B2 (en) Multiple analyte assay devices
US5817522A (en) Self-contained assay device and method
US5620657A (en) Device and method for completing a fluidic circuit
US5427739A (en) Apparatus for performing immunoassays
US8623291B2 (en) Multiple analyte assay device
JP3007410B2 (en) Multiwell test
CA2309504C (en) Self-contained assay device and method
AU2003262462B2 (en) Multiple analyte assay device

Legal Events

Date Code Title Description
MKLA Lapsed