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Publication numberCA1187786 A
Publication typeGrant
Application numberCA 399130
Publication date28 May 1985
Filing date23 Mar 1982
Priority date24 Mar 1981
Also published asCA1187786A1, DE3111474A1, EP0061167A1, EP0061167B1
Publication numberCA 1187786 A, CA 1187786A, CA 399130, CA-A-1187786, CA1187786 A, CA1187786A
InventorsRobert Ziegelmaier
ApplicantRobert Ziegelmaier, Behringwerke Aktiengesellschaft, Behring Diagnostics Gmbh, Dade Behring Marburg Gmbh
Export CitationBiBTeX, EndNote, RefMan
External Links: CIPO, Espacenet
Agent for immunological diagnosis and a process for its preparation
CA 1187786 A
Abstract
Abstract of the Disclosure A process for preparing a carrier for use in the detection and determination of a partner of an immunological reaction, the associated partner being on the surface of the carrier, in which the carrier is irradiated with gamma radiation before or after the associated partner is bound thereto. A polymeric carrier produced by the process is also disclosed.
Claims(5)
THE EMBODIMENTS OF THE INVENTION IN WHICH AN EXCLUSIVE
PROPERTY OR PRIVILEGE IS CLAIMED ARE DEFINED AS FOLLOWS:
1. A process for preparing a polymeric carrier for use in the detection and determination of an immunological reaction partner present in a liquid contacted with the carrier, the carrier having the associated partner bound thereto, which process comprises irradiating the carrier with gamma radiation before or after the associated partner is bound to the carrier.
2. A process as claimed in claim 1 wherein the carrier is irradiated with from 0.1 to 100 mégarads of gamma radiation.
3. A process as claimed in claim 2 wherein the carrier is irradiated with from 0.5 to 10 megarads of gamma radiation.
4. A process as claimed in claim 1 wherein said partner bound to the carrier is an antigen or antibody.
5. A process as claimed in claim 1 wherein said polymeric carrier is of polystyrene or polyvinyl chloride.
Description  (OCR text may contain errors)

The invention relates to a carrier for the detection and determination of an immunological reaction partner present in a liquid, the associated partner being on the surface of the c,^arrier.
It is known to bind a partner of an immunolo~ical reaction adsorptively or covalently to a carrier in order to isolate the corresponding partner from a solution in or-der to detect this partner or to determine its amount.
However, it is al50 known that test methods based On this principle can have deficiencies.
Thus, Chessum and Denmark describe, in The Lancet (1978), page 161, that the known polystyrene microtiter plates used as the carrier in a process for the deter-mination of antigens or antibodies which is generally called ELISA (Enzyme Linked Immuno Sorbent Assay) can lead to intolerably large variations in the test result.
Bidwell et al. also report, in J. Infect. Disease 136, Supplement, page 27~ (1977), variations in the results of an ELISA test which it was possible to attribute to differences in the microtiter plates used as the carrier.
These wide varia-tions in the test result which were caused by differences in the surface properties of the microtiter plates should not be restricted to the use of these plates, but should also occur with carriers of a different shape made from the same materials.
Accordingly, there was the object of discovering '7'7~

carrier materials which have more uniform surface pro-perties and which, when used, give immunological test results which vary less.
Surprisingly, it has now been Eound that this object can be achieved by irradiating the carrier material, and preferably microtiter plates prepared therefrom, with gamma rays, preferably with cobalt rays, and preferably with 0.1 to 100, in particlular 0.5 to 10, megarads.
The invention thus relates to a process for preparing a polymeric carrier for the detection and determination o~ a partner o an immunological reaction in which the corresponding partner is on the surface of the carrier, wherein the carrier is irradiated, before or after its surface has been coated with this partner.
It is not necessary to coat the carrier immediately after irradiation. The advantageous effect of irradiation is also observed if a period of days or months elapses between irradiation and coating.
In principle, all plastics, but in particular polystyrene and polyvinyl chlcride~ can be used as carrier materials in this process.
Irradiated carriers can advantageously be used in immune test systems which are known per se, for example immune test systems based on an antigen/antibody reaction, for example ELISA systems (Enzyme Linked Immuno Sorbent Assay systems), a bonding partner being bonded to the irradiated 77~

carrier.
Such bonding partners can be, for example, proteins, such as immunoglobulins, antimicrobial anti-bodies, plasma proteins, for example Clq, or antigenic materials, for example allergens or microbial antigens.
The example whi.ch follows illustrates the invention:
EXAMPLE
Preparation of a test kit for the determination of anti-bodies of the IgG class and IgM class against mumps virus in human serum.
1. Bonding of the virus antigen to the carrier (polystyrene microtiter plate) a. irradiated (about 2 mrad~
b. not irradiated 15 2. Washing 3. Application and incubation of serum dilutions 4. Washing 5. Application and incubation of anti-human IgG- or IgM-alkaline phosphatase conjugate 20 6. Washing 7. Application of the substrate solution 8. Stopping of enzymatic splitting by addition of 2N NaOH
9. Photometric measurement at 405 nm ~7~

Table Comparison of the sensitivity of a test with an irradi-ated carrier with that of a test with a non-irradiated carrier:

Detection limit (titer 1:) irradiated carrier non-irradiated carrier Serum IgG IgM ~ IgG I~M
A 20,480 20,480 1,280 2,560 10 3 1,280 160 640 The figures show the advantage of using irradi-ated carriers. The detectability (sensitivity) is significantly increased.

Referenced by
Citing PatentFiling datePublication dateApplicantTitle
US5378638 *20 May 19933 Jan 1995Boehringer Mannheim GmbhAnalysis element and process for its manufacture
US5424220 *29 Jan 199313 Jun 1995Boehringer Mannheim GmbhAnalysis element and method for determination of an analyte in a liquid sample
Classifications
International ClassificationG01N33/543, A61K41/00
Cooperative ClassificationG01N33/54393, A61K41/0023
Legal Events
DateCodeEventDescription
28 May 2002MKEXExpiry