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Glioma invasion mediated by the p75 neurotrophin receptor (p75 NTR /CD271 ...
PDLIM1 is a member of the ALP subfamily of PDZ/LIM proteins, which are postulated to have a role in actin cytoskeleton architecture.46, 49, 50, 51, 52, 53, 54. There is mounting evidence that the neurotrophin receptor family and their respective ...
Rheostatic Regulation of the SERCA/Phospholamban Membrane Protein Complex ...
Remarkably, longer oligonucleotides result in complete inhibition relief, an effect that mimics phosphorylation of PLN at Ser16 by protein kinase A. With oligonucleotide sequences longer than 50 bases, we observed no further increase of SERCA activity ...
Derivation of Ethnically Diverse Human Induced Pluripotent Stem Cell Lines
(c) 48-hours after infection, less than 15% of fibroblasts were immunopositive for viral-derived Oct4 by immunocytology (scale bar is 50 μm). (d,f) After 35 days, infected ED fibroblasts generated stem cell-like colonies that were .... Briefly, after ...
Prolyl-isomerase Pin1 controls Notch3 protein expression and regulates T-ALL ...
The follow-up of mice was stopped at 24 weeks, being 75% of the N3IC-tg mice dead at this age and 70% of N3IC-tg/Pin1−/− mice survivor (n=50 for Pin1+/+; n=60 for N3IC-tg n=30 for N3IC-tg/Pin1−/− mice). Full figure and ..... The studies involving ...
TRM6/61 connects PKCα with translational control through tRNA i Met ...
Accordingly, in mammalian C6 cells, small interfering RNA (siRNA)-mediated depletion of either TRM6 or TRM61, or both (Figure 2a) decreased significantly cell growth (Figure 2b) and caused up to 50% of cell death following 72 h as visualized by annexin ...
LZTFL1 suppresses lung tumorigenesis by maintaining differentiation of lung ...
Tissue sections (5 μm) were stained with anti-LZTFL1 antibody and assigned expression scores—staining intensity (0, no staining; 1, weak; 2, medium; 3 strong) multiplied by the proportion of stained cells (1, <50% stained; 2, >50%stained) as described ...
1 Institute of Molecular and Cellular Biology, National Tsing Hua University ...
(a) Signaling pathways affecting E-cadherin endocytosis were examined by pretreating LM cells with PP2 (30 μM), LY294002 (50 μM), PD98059 (50 μM), GF109203X (5 μM) or vehicle for 30 min, followed by Wnt3a treatment. ..... Briefly, cells were grown in ...
Safety and biodistribution assessment of sc-rAAV2.5IL-1Ra administered via ...
Concentrations of vector genome in the blood were below the limit of quantitation (50 vg/�g genomic DNA) at 4 hours or 24 hours post-vector injection in all the control and low-dose vector animals, as well as in mid-dose vector males and some females ...
RNA helicase DDX3: a novel therapeutic target in Ewing sarcoma
(b and c) Cohorts of 5–7 NSG mice were implanted with primary ESFT sarcoma xenografts in the subcutaneous flank; treatment with 50 mg/kg RK-33 or vehicle (DMSO) intraperitoneally five times weekly was initiated at the time tumors became palpable ...
Estimating cell diffusivity and cell proliferation rate by interpreting ...
Cells are routinely propagated in RPMI 1640 medium (Life Technologies, Australia) in 10 % foetal calf serum (Sigma-Aldrich, Australia), with 110 u/mL penicillin, 100 μg/mL streptomycin (Life Technologies), in plastic flasks (Corning Life Sciences, Asia ...